Engineered strain of Escherichia coli for production of poly-R-3-hydroxyalkanoate polymers with defined monomer unit composition and methods based thereon

ABSTRACT

Methods and systems for producing prescribed unit size azido-poly(3-hydroxyalkanoate) (azido-PHA) polymers and copolymers are provided. The methods and systems can employ recombinant bacteria that are not native producers of PHA or lack enzymes to degrade PHA once synthesized, metabolize short to long chain fatty acids without induction, and express an (R)-specific enoyl-CoA hydratase and a PHA synthase, the (R)-specific enoyl-CoA hydratase and PHA synthase having wide substrate specificities. The recombinant bacteria are fed at least one ω-azidofatty acid substrate that is equal in carbon length to the prescribed or desired unit size of an azido-PHA polymer to be produced. Azido-PHA polymers or azido-PHA co-polymers can be conjugated via copper-catalyzed alkyne-azide cycloaddition (CuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) reactions. The prescribed unit size conjugated azido-PHA polymer or orthogonally conjugated azido-PHA co-polymer that is produced is then isolated and/or purified.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation-in-part of co-pending U.S. patent application Ser. No. 14/371,025 entitled “Engineered Strain of Escherichia coli for Production of Poly-R-3-Hydroxyalkanoate Polymers with Defined Monomer Unit Composition and Methods Based Thereon,” which is a national stage application under 35 U.S.C. § 371 of PCT Application No. PCT/US2013/020875, which claims priority to and the benefit of U.S. provisional patent application Ser. No. 61/584,495, entitled “Engineered Strain of Escherichia coli for Production of R-3-Hydroxyalkanoic Acids and Poly-R-3-Hydroxyalkanoate Polymers with Defined Monomer Unit Composition,” filed Jan. 9, 2012, each of which is incorporated herein by reference in its entirety.

STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT

This invention was made with government support under award CBET1263905 awarded by the National Science Foundation. The government has certain rights in the invention.

1. TECHNICAL FIELD

The present invention relates to biodegradable poly(3-hydroxyalkanoate) polymers and copolymer and methods for synthesizing biodegradable poly(3-hydroxyalkanoate) polymers and copolymers.

2. BACKGROUND OF THE INVENTION

Poly(3-hydroxyalkanoates) (PHAs) are biodegradable plastics and carbon storage materials that are synthesized by a myriad of microorganisms. PHAs are divided into groups based on repeating unit size. PHAs with repeating units of three to five carbons in length are short-chain-length (SCL) PHAs, and PHAs with repeating units of six to fourteen carbons in length are medium-chain-length (MCL) PHAs. Differences in repeating unit composition influence the physical properties of PHAs. SCL PHA homopolymers such as poly-3-hydroxybutyrate (PHB) have been previously produced, but methods to control the repeating unit composition of the MCL PHAs have been limited. Previous attempts to control MCL PHA synthesis in native and recombinant PHA-producing organisms have resulted in narrow ranges of repeating unit control. Typically, control is limited to only a couple of repeating units within one organism or control is lost once the number of carbons in the repeating unit exceeds seven

Thus, the composition of medium-chain-length (MCL) poly(3-hydroxyalkanoate) (PHA) biopolymers is normally an uncontrollable random mixture of repeating units with differing side chain lengths. Attempts to generate MCL PHA homopolymers and gain control of the repeating unit composition have been reported for native or natural PHA-producing organisms but have limited ranges for the different sizes of repeating units that can be synthesized.

Citation or identification of any reference in Section 2, or in any other section of this application, shall not be considered an admission that such reference is available as prior art to the present invention.

3. SUMMARY OF THE INVENTION

A method is provided for producing a prescribed unit size poly(3-hydroxyalkanoate) (PHA) polymer comprising the steps of:

-   -   providing a recombinant bacterium, wherein the bacterium:         -   metabolizes short to long chain fatty acids without             induction, and         -   expresses an (R)-specific enoyl-CoA hydratase and a PHA             synthase;     -   feeding at least one fatty acid substrate to the recombinant         bacterium, the at least one fatty acid substrate being of equal         carbon length to the prescribed unit size of the PHA polymer to         be produced; and     -   isolating and/or purifying the PHA from the recombinant         bacterium, thereby producing the PHA polymer.

A prescribed unit size refers to the desired number of carbon atoms that are in the unit.

In one embodiment of the method, the (R)-specific enoyl-CoA hydratase and PHA synthase have wide substrate specificities.

In another embodiment of the method, the PHA polymer is a PHA homopolymer, a PHA copolymer or a PHA-co-polylactic acid (PLA) polymer.

In another embodiment of the method, at least one lactic acid substrate is co-fed to the bacterium and a PHA-co-PLA polymer is produced.

In another embodiment of the method, the recombinant bacterium is a recombinant E. coli.

In another embodiment of the method, the step of providing a recombinant bacterium comprises the step of constructing a plasmid comprising an (R)-specific enoyl-CoA hydratase gene and a PHA synthase gene and transforming the plasmid into the recombinant bacterium.

In another embodiment of the method, the recombinant bacterium is a recombinant E. coli and the plasmid is pBBR-C1J4SII.

In another embodiment of the method, β-oxidation is blocked in the recombinant bacterium, thereby causing a buildup of enoyl-CoA intermediates.

In another embodiment of the method, at least one gene encoding an enzyme for β-oxidation is inactive or deleted from the chromosome of the recombinant bacterium.

In another embodiment of the method, the at least one gene encoding an enzyme for β-oxidation is a gene encoding an (S)-specific enoyl-CoA hydratase.

In another embodiment of the method, the at least one gene encoding an enzyme for β-oxidation is a fad gene.

In another embodiment of the method, the recombinant bacterium is recombinant E. coli and an (S)-specific enoyl-CoA hydratase fadB gene and/or a fadJ gene is deleted from the chromosome of the recombinant E. coli.

In another embodiment of the method, the recombinant bacterium constitutively expresses a positive regulator of an operon, wherein the operon promotes uptake of short chain fatty acids for β-oxidation.

In another embodiment of the method, the recombinant bacterium is a recombinant E. coli and wherein the recombinant E. coli constitutively expresses a positive regulator of the ato operon, thereby promoting uptake of short chain fatty acids for β-oxidation.

In another embodiment of the method, the recombinant E. coli constitutively expresses AtoC.

In another embodiment of the method, the recombinant bacterium is a recombinant E. coli, the (R)-specific enoyl-CoA hydratase is PhaJ4 and the PHA synthase is PhaC1(STQK).

In another embodiment of the method, the recombinant bacterium expresses propionyl-CoA transferase (PCT).

In another embodiment of the method, the recombinant bacterium has gene deletions for a negative transcriptional regulator of the β-oxidation pathway.

In another embodiment of the method, the negative transcriptional regulator is arcA or ompR.

In another embodiment of the method, the recombinant bacterium has gene deletions for arcA and ompR.

In another embodiment of the method, at least two different types of fatty acid substrates of desired length are fed to the recombinant bacteria in a desired ratio, thereby producing a PHA polymer, PHA copolymer or PHA-co-PLA polymer of desired (or controlled) repeating unit composition.

A method for producing the recombinant bacterium is also provided, wherein the bacterium:

-   -   metabolizes short to long chain fatty acids without induction,         and     -   expresses an (R)-specific enoyl-CoA hydratase and a PHA         synthase.

In one embodiment of the method, the recombinant bacterium is not a native or natural producer of PHA or lacks enzymes to degrade PHA once synthesized.

In another embodiment the method, the (R)-specific enoyl-CoA hydratase and PHA synthase have wide substrate specificities.

A system is provided for producing a prescribed unit size poly(3-hydroxyalkanoate) (PHA) polymer comprising a recombinant (genetically engineered) bacterium, wherein the bacterium:

-   -   metabolizes short to long chain fatty acids without induction,         and     -   expresses an (R)-specific enoyl-CoA hydratase and a PHA         synthase.

A prescribed unit size refers to the desired number of carbon atoms that are in the unit.

In one embodiment of the system, the PHA polymer is a PHA homopolymers, a PHA copolymer or a PHA-co-PLA polymer.

In another embodiment of the system, the (R)-specific enoyl-CoA hydratase and PHA synthase have wide substrate specificities.

In another embodiment of the system, the recombinant bacterium is a recombinant E. coli.

In another embodiment of the system, the recombinant bacterium comprises a plasmid comprising an (R)-specific enoyl-CoA hydratase gene and a PHA synthase gene.

In another embodiment of the system, the recombinant bacterium is a recombinant E. coli and the plasmid is pBBR-C1J4SII.

In another embodiment of the system, β-oxidation is blocked in the recombinant bacterium, thereby causing a buildup of enoyl-CoA intermediates.

In another embodiment of the system, at least one gene encoding an enzyme for β-oxidation is inactive or deleted from the chromosome of the recombinant bacterium.

In another embodiment of the system, the at least one gene encoding an enzyme for β-oxidation is a gene encoding an (S)-specific enoyl-CoA hydratase.

In another embodiment of the system, the at least one gene encoding the enzymes for β-oxidation is a fad gene.

In another embodiment of the system, the recombinant bacterium is recombinant E. coli and an (S)-specific enoyl-CoA hydratase fadB gene and/or a fadJ gene is deleted from the chromosome of the recombinant E. coli.

In another embodiment of the system, the recombinant bacterium constitutively expresses a positive regulator of an operon, wherein the operon promotes uptake of short chain fatty acids for β-oxidation.

In another embodiment of the system, the recombinant bacterium is a recombinant E. coli and wherein the recombinant E. coli constitutively expresses a positive regulator of the ato operon, thereby promoting uptake of short chain fatty acids for β-oxidation.

In another embodiment of the system, the recombinant E. coli constitutively expresses AtoC.

In another embodiment of the system, the recombinant bacterium is a recombinant E. coli, the (R)-specific enoyl-CoA hydratase is PhaJ4 and the PHA synthase is PhaC1(STQK).

In another embodiment of the system, the recombinant bacterium expresses propionyl-CoA transferase (PCT).

In another embodiment of the system, the recombinant bacterium has gene deletions for a negative transcriptional regulator of the β-oxidation pathway.

In another embodiment of the system, the negative transcriptional regulator is arcA or ompR.

In another embodiment of the system, the recombinant bacterium has gene deletions for arcA and ompR.

Recombinant bacteria and genetic constructs for producing the bacteria are also provided.

A recombinant bacterium is provided, wherein the bacterium:

-   -   metabolizes short to long chain fatty acids without induction,         and     -   expresses an (R)-specific enoyl-CoA hydratase and a PHA         synthase.

In one embodiment of the recombinant bacterium, the recombinant bacterium is a recombinant E. coli.

In another embodiment of the recombinant bacterium, the (R)-specific enoyl-CoA hydratase and PHA synthase have wide substrate specificities.

In another embodiment of the recombinant bacterium, the bacterium comprises a plasmid comprising an (R)-specific enoyl-CoA hydratase gene and a PHA synthase gene.

In another embodiment of the recombinant bacterium, the recombinant bacterium is a recombinant E. coli and the plasmid is pBBR-C1J4SII.

In another embodiment of the recombinant bacterium, β-oxidation is blocked in the recombinant bacterium.

In another embodiment of the recombinant bacterium, at least one gene encoding an enzyme for β-oxidation is inactive or deleted from the chromosome of the recombinant bacterium.

In another embodiment of the recombinant bacterium, the at least one gene encoding an enzyme for β-oxidation is a gene encoding an (S)-specific enoyl-CoA hydratase.

In another embodiment of the recombinant bacterium, the at least one gene encoding an enzyme for β-oxidation is a fad gene.

In another embodiment of the recombinant bacterium, the recombinant bacterium is recombinant E. coli and an (S)-specific enoyl-CoA hydratase fadB gene and/or a fadJ gene is deleted from the chromosome of the recombinant E. coli.

In another embodiment of the recombinant bacterium, the recombinant bacterium constitutively expresses a positive regulator of an operon, wherein the operon promotes uptake of short chain fatty acids for β-oxidation.

In another embodiment of the recombinant bacterium, the recombinant bacterium is a recombinant E. coli and wherein the recombinant E. coli constitutively expresses a positive regulator of the ato operon, thereby promoting uptake of short chain fatty acids for β-oxidation.

In another embodiment of the recombinant bacterium, the recombinant E. coli constitutively expresses AtoC.

In another embodiment of the recombinant bacterium, the recombinant bacterium is a recombinant E. coli, the (R)-specific enoyl-CoA hydratase is PhaJ4 and the PHA synthase is PhaC1(STQK).

In another embodiment of the recombinant bacterium, the recombinant bacterium expresses propionyl-CoA transferase (PCT).

In another embodiment of the recombinant bacterium, the recombinant bacterium has gene deletions for a negative transcriptional regulator of the β-oxidation pathway.

In another embodiment of the recombinant bacterium, the negative transcriptional regulator is arcA or ompR.

In another embodiment of the recombinant bacterium, the recombinant bacterium has gene deletions for arcA and ompR.

A plasmid (or vector) is provided comprising an (R)-specific enoyl-CoA hydratase gene and a PHA synthase gene.

In one embodiment of the plasmid, the plasmid comprises phaJ4 and phaC1(STQK).

In another embodiment of the plasmid, the (R)-specific enoyl-CoA hydratase and PHA synthase have wide substrate specificities.

In another embodiment of the plasmid, the plasmid is pBBR-C1J4SII.

In another embodiment of the plasmid, the plasmid comprises a pct gene.

A SCL or MCL PHA is also provided comprising a fatty acid with a terminal vinyl group (alkene and/or alkyne).

A method is also provided for producing a prescribed unit size azido-poly(3-hydroxyalkanoate) (azido-PHA) polymer comprising:

-   -   (a) providing a recombinant bacterium, wherein the bacterium:         -   i. metabolizes short to long chain fatty acids without             induction, and         -   ii. expresses an (R)-specific enoyl-CoA hydratase and a             poly(3-hydroxyalkanoate) (PHA) synthase;     -   (b) feeding to the recombinant bacterium a fatty acid substrate,         wherein the fatty acid substrate is a ω-azidofatty acid         substrate, the ω-azidofatty acid comprising a terminal azide,         and the ω-azidofatty acid being of equal carbon length to the         prescribed unit size of the azido-PHA polymer to be produced;         and     -   (c) isolating and/or purifying the azido-PHA polymer from the         recombinant bacterium, thereby producing the prescribed unit         size azido-PHA polymer.

In one embodiment of the method, the bacterium is not a native or natural producer of PHA or lacks enzymes to degrade PHA once synthesized.

In another embodiment of the method, the PHA polymer is a PHA homopolymer, a PHA copolymer or a PHA-co-polylactic acid (PLA) polymer.

In another embodiment, the method comprises, after (c),

-   -   conjugating the azido-PHA polymer via a copper-catalyzed         alkyne-azide     -   cycloaddition (CuAAC) reaction or a strain-promoted azide-alkyne         cycloaddition reaction (SPAAC) with alkynes,         thereby producing a conjugated PHA polymer or an orthogonally         conjugated PHA co-polymer.

In another embodiment of the method, (b) further comprises feeding at least one of 3-hydroxybutyrate, 3-hydroxyoctanoate, or 3-hydroxydodecanoate monomer to the recombinant bacterium, and after (c), a conjugated PHA polymer or an orthogonally conjugated PHA co-polymer is produced.

In another embodiment of the method, the recombinant bacterium is a recombinant E. coli.

In another embodiment of the method, at least two different types of fatty acid substrate of desired length are fed to the recombinant bacteria in a desired ratio, and wherein at least one of the two different types of fatty acid substrate comprises a terminal azide moiety, thereby producing an azidoPHA homopolymer, azido-PHA copolymer of desired or controlled repeating unit composition.

A system is also provided for producing a prescribed unit size azido-poly(3-hydroxyalkanoate) (azido-PHA) polymer comprising a recombinant bacterium, wherein the bacterium:

-   -   a. metabolizes short to long chain fatty acids without         induction, and     -   b. expresses an (R)-specific enoyl-CoA hydratase and a PHA         synthase.

In one embodiment of the system, the bacterium is not a native or natural producer of PHA or lacks enzymes to degrade PHA once synthesized.

In another embodiment of the system, the recombinant bacterium is a recombinant E. coli.

In another embodiment of the system, the recombinant bacterium comprises a plasmid comprising an (R)-specific enoyl-CoA hydratase gene and a PHA synthase gene.

A copolymer in the form of PHA_(scl/mcl)co-PHX is also provided.

A copolymer in the form of PHA_(scl/mcl)co-PHX-co-PHX is also provided.

4. BRIEF DESCRIPTION OF THE DRAWINGS

The present invention is described herein with reference to the accompanying drawings, in which similar reference characters denote similar elements throughout the several views. It is to be understood that in some instances, various aspects of the invention may be shown exaggerated, enlarged, exploded, or incomplete to facilitate an understanding of the invention.

FIG. 1. Production of various poly[(R)-3-hydroxyalkanoates] (PHAs) in Escherichia coli LSBJ. The β-oxidation pathway of Escherichia coli LS5218 was altered to obtain repeating unit control in synthesis of poly(3-hydroxyalkanoates) (PHAs). The gene for the (S)-specific enoyl-CoA hydratase fadB and its homologue fadJ (formerly yfcX) were eliminated on the chromosome of E. coli LS5218. The new strain was named E. coli LSBJ. An (R)-specific enoyl CoA hydratase (PhaJ4) and PHA synthase [PhaC1(STQK)] can convert fatty acids that enter the β-oxidation pathway to PHAs at the enoyl-CoA step. The fadB and fadJ gene knockouts prevent degradation of the substrate via carbon removal, and subsequent PHAs produced contain repeating units with the same number of carbons as the starting fatty acid substrate. By feeding various ratios of fatty acids to the strain, production of PHA copolymers with specific repeating unit compositions and physical properties were achieved. See Examples 1 and 2 for details.

FIGS. 2A-B. (A) Map of Escherichia coli genome at the genes fadB and fadJ before and after removal of the genes. The knockout of fadB was carried out by the insertion of a chloramphenicol acetyltransferase (cat). The knockout of fadJ was carried out by insertion of a neomycin phosphotransferase and subsequent removal, leaving behind a short scar sequence. The numbers next to each map represents approximate PCR product sizes with the “check” primers in Table 2. Half arrows indicate approximate ligation sites for primers a (fadB.F.check), b (fadB.R.check), x (fadJ.F.check), and y (fadJ.R.check). (B) Agarose electrophoresis gel of PCR products of fadB and fadJ regions of E. coli LS5218 and E. coli LSBJ. Lane 1, 1 kb ladder (New England Biolabs); Lane 2, PCR from E. coli LS5218 using fadB.F.check and fadB.R.check primers; Lanes 3 and 4, PCR from E. coli LSBJ using fadB.F.check and fadB.R.check primers; Lane 5, PCR from E. coli LS5218 using fadJ.F.check and fadJ.R.check primers; and Lanes 6 and 7, PCR from E. coli LSBJ using fadJ.F.check and fadJ.R.check primers. See Examples 1 and 2 for details.

FIG. 3. H-¹³C HSQC-DEPT NMR (nuclear magnetic resonance) spectroscopy results for poly(3-hydroxyoctanoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. See Example 1 for more details.

FIG. 4. ¹H-¹³C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxydecanoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. See Example 1 for more details.

FIG. 5. H-¹³C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxy-10-undecenoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. See Example 1 for more details.

FIG. 6. ¹H-¹³C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxydodecanoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. See Example 1 for more details.

FIGS. 7A-B. Thermal analysis of purified medium-chain-length poly(3-hydroxyalkanoates). See Example 1 for more details.

FIG. 8. 300 MHz 1H NMR spectrum of a poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] sample synthesized in E. coli LSBJ. Cells for this sample were grown in the presence of sodium octanoate and sodium butyrate at a mole ratio of 10:90 to generate a copolymer consisting of two repeating units with eight carbons and four carbons, respectively. 3HO, (R)-3-hydroxyoctanoate; 3HB, (R)-3-hydroxybutyrate. Based on the peak integrals, this polymer sample was determined to have a 21.8:78.2 mole ratio of 3HO to 3HB. FIG. 10 (below) shows 1H-13C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxyoctanoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. See Example 2 for more details.

FIGS. 9A-D Summary of tensile strength analyses for various poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] samples. A) Young's modulus B) strain to failure C) yield strength D) ultimate tensile strength. The x-axes of all charts represent repeating unit ratios of (R)-3-hydroxyoctanoate to (R)-3-hydroxybutyrate in the polymer samples. Values represent averages of between 4-7 experiments plus or minus standard deviations about those averages.

FIG. 10. 1H-13C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxyoctanoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. See Example 2 for more details.

FIG. 11. 1H-13C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxydecanoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid.

FIG. 12. 1H-13C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxy-10-undecenoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. See Example 2 for more details.

FIG. 13. 1H-13C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxydodecanoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. See Example 2 for more details.

FIGS. 14A-B. Thermal analysis of purified medium-chain-length poly(3-hydroxyalkanoates). See Example 2 for more details.

FIG. 15. The mol ratio of repeating units in poly[(R)-3-hydroxydodecanoate-co-(R)-3-hydroxydecanoate] polymer samples in relation to the starting fatty acid substrate ratio. See Example 2 for more details.

FIG. 16. The mol ratio of repeating units in poly[(R)-3-hydroxydodecanoate-co-(R)-3-hydroxyoctanoate] polymer samples in relation to the starting fatty acid substrate ratio. See Example 2 for more details.

FIG. 17. The mol ratio of repeating units in poly[(R)-3-hydroxydecanoate-co-(R)-3-hydroxyoctanoate] polymer samples in relation to the starting fatty acid substrate ratio. See Example 2 for more details.

FIG. 18. The mol ratio of repeating units in poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] polymer samples in relation to the starting fatty acid substrate ratio. See Example 2 for more details.

FIG. 19. TGA (thermogravimetric analysis) scans of various poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] samples synthesized in E. coli LSBJ containing the pBBR-C1J4SII plasmid. See Example 2 for more details.

FIG. 20. DSC scans of various poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] samples synthesized in E. coli LSBJ containing the pBBR-C1J4SII plasmid. See Example 2 for more details.

FIG. 21. Examples of data generated in tensile strength analysis experiments with poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] samples. See Example 2 for more details.

FIG. 22. Fitted line plot of the natural logarithm of Young's modulus values (LN_Yng) against the percent of 3HO (C8_Proportion) in of poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] samples. LN_Yng=5.724-0.1157 Plot was made in the MINITAB software program. See Example 2 for more details.

FIG. 23. Fitted line plot of the natural logarithm of ultimate tensile strength values (LN_Tens) against the percent of 3HO (C8_Proportion) in of poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] samples. LN_Tens=2.779-0.07740. Plot was made in the MINITAB software program. See Example 2 for more details.

FIGS. 24A-C. 1H NMR spectra of poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] containing 68.7% PHB prior to and after acetone fractionation. A, sample spectra prior to acetone fractionation. B, acetone soluble fraction. C, acetone insoluble fraction. See Example 2 for more details.

FIG. 25. DSC results of poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] containing 68.7% PHB before and after acetone fractionation. Scans were offset for clarity. The top scan is of the sample prior to acetone fractionation. The middle scan is the acetone soluble fraction. The bottom scan is the acetone insoluble fraction.

FIG. 26. Synthetic pathways for the production of PHA polymers from fatty acids in E. coli. A. Fatty acids are activated for degradation by the acyl-CoA ligase, FadD where they enter the β-oxidation pathway. B. 3-Ketoacyl reductases such as FabG and RhlG can be overexpressed to convert 3-ketoacyl-CoA to (R)-3-hydroxyacyl-CoA. C. (R)-specific enoyl-CoA hydratases can be overexpressed to convert enoyl-CoA to (R)-3-hydroxyacyl-CoA. D. PhaC polymerizes the precursors generated by the two pathways into PHA.

FIG. 27. Engineering E. coli to produce PHA polymers with defined repeating unit compositions. Several modifications in this strain result in the production of PHA polymers with defined repeating unit compositions: (i) fadR mutation results in constitutive expression of the fad genes, (ii) atoC(Con) results in constitutive expression of the ato genes, (iii) deletion of the fadB and fadJ genes results in the pooling of enoyl-CoA intermediates of defined carbon lengths from fed fatty acids, (iv) expression of phaJ results in the conversion of specific enoyl-CoA substrates to (R)-3-hydroxyacyl-CoA substrates, and (v) expression of phaC1(STQK) results in polymerization of the (R)-3-hydroxyacyl-CoA substrates into PHA polymers dependent on the ratio of fatty acids fed to the strain.

FIG. 28. The mol ratio of repeating units in poly[(R)-3-hydroxyoctanoateco-(R)-3-hydroxybutyrate] polymer samples in relation to starting fatty acid substrate ratios. The fatty acid substrates were used by E. coli LSBJ. All values were determined by 1H NMR and represent average integrations of three separate peaks in the 1H NMR spectra. These integrations were compared to the integration of a peak unique to the 8-carbon repeating unit. Error bars represent standard deviations about those averages.

FIG. 29. Gene rearrangement to control molecular weights of MCL PHA-co-PLA polymers. Synthetic operons for MCL PHA-co-PLA polymer production will be made via Gibson assembly. Overlaying primers will be designed to rearrange all monomer-supplying genes (pct, phaG, and alkK) relative to the PHA synthase gene encoding phaC1(STQK) shown here as phaC1.

FIG. 30. Engineering transcriptional regulators in E. coli for enhanced fatty acid uptake. Regulation of the E. coli fad regulon responds to several environmental stimuli including: long chain fatty acids (LCFA), glucose, oxygen, and osmotic stress. These external stimuli influence a number of regulatory proteins (in bold) including FadR, catabolite repression protein, ArcA-P, and OmpR. The fad regulon components are in italics and are either positively regulated as indicated by a black arrow “↓” or repressed as denoted by a gray “⊥”.

FIG. 31. Engineering E. coli for precise control of MCL PHA and PLA repeating unit composition. E. coli LSBJ has mutations [atoC(Con), fadR, fadB, fadJ] for the acquisition and conversion of small organic acids like lactic acid and fatty acids. With the co-expression of the (R)-specific enoyl-CoA hydratase, PhaJ, PHA synthase, PhaC, and propionyl-CoA transferase, PCT, the strain can easily be engineered for the production of PHA-co-PLA polymers. The ratio of PHA to PLA incorporated into the copolymer will be dependent on the ratio of feedstocks (fatty acids:lactic acid) available to the strain. By feeding different ratios of lactic acid and fatty acid to the cells and determining the polymer composition, copolymers with desired ratios of PHA to PLA repeating units can be made. The system shown here is for MCL PHA-co-PLA polymer production but can be readily adapted for the production of SCL PHA-co-PLA polymers with desired repeating unit compositions.

FIG. 32. Poly[(R)-3-hydroxy-10-undecenoate-co-(R)-3-hydroxyoctanoate] production in E. coli LSBJ harboring the pBBR-C1J4SII plasmid. The (R)-3-hydroxy-10-undecenoate repeating units were provided by 10-undecenoate, and the (R)-3-hydroxyoctanoate repeating units were provided by sodium octanoate. The relative concentration of fatty acid substrates was varied to demonstrate the corresponding change in terminal alkenes present in the polymer product (determined by NMR). This plot portrays the change in concentration of terminal alkenes in repeating units of PHAs as a function of starting concentration of 10-undecenoate relative to total fatty acid content.

FIG. 33. 300 MHz ¹H NMR spectrum of a poly[(R)-3-hydroxy-10-undecenoate-co-(R)-3-hydroxyoctanoate] sample synthesized by E. coli LSBJ carrying the pBBR-C1J4SII plasmid. Cells were grown with 10-undecenoate and sodium octanoate at a mole ratio of 50:50 to generate this sample. 3HUnΔ10, (R)-3-hydroxy-10-undecenoate; 3HO, (R)-3-hydroxyoctanoate. Based on the peak integrations, it was determined that this polymer had a 3HUnΔ10:C8 mole ratio of 72.3:27.7.

FIG. 34. Scheme 1. Synthetic routes to ω-azidofatty acids.

FIGS. 35A-B. Structural characterization of purified PHODN₃. (A) FT-IR (Fourier transform infrared spectroscopy) spectrum highlighting the characteristic sharp asymmetric stretch of azide moieties (shown in red in structure). (B) 600 MHz ¹H NMR spectrum of a PHODN₃ sample biosynthesized by E. coli LSBJ. Cells for this sample were grown in the presence of sodium octanoate and 10-azidodecanoic acid at a mole ratio of 1:0.111 to generate a copolymer consisting of two repeating units with eight carbons and ten carbons, respectively. Peak integrations were normalized (a value of 3.0) to the upfield triplet visible at 0.88 ppm corresponding to the polymer's terminal methyl group (C8-8). Based on the peak integral for C10N₃-10 (shown in red), this polymer sample was determined to have a 1:0.240 C8:C10N₃ mol ratio.

FIGS. 36A-B. SPAAC of PHODN₃ (1:0.33 C8:C10N₃) with commercially available BCN—OH (11). (A)¹H NMR of unreacted PHODN₃. (B)¹H NMR of PHODN₃-BCN—OH.

FIG. 37. Azido-PHAs are anticipated to expand the application spectrum of these important biopolymers into previously untapped biomedical and material science applications.

FIG. 38. Nanoparticle design for drug delivery employing novel click-ready PHAs.

FIG. 39. Production of Azido-PHA-DBCO-5

FIG. 40. Right panel is a photomicrograph showing that Azido-PHA-DBCO-5 nanoparticles are shown to be preferentially incorporated into MDA-MB231 cells (P13). Left panel is a photomicrograph showing the control.

FIG. 41. NMR assignment and structural confirmation of PHODN₃-BCN—OH: ¹H NMR

FIG. 42. NMR assignment and structural confirmation of PHODN₃-BCN—OH: COSY.

FIG. 43. NMR assignment and structural confirmation of PHODN₃-BCN—OH: HSQC with adiabatic bilevel decoupling.

FIG. 44. NMR assignment and structural confirmation of PHODN₃-BCN—OH: 1D TOCSY (irradiated from =3.78-3.63 ppm).

FIGS. 45A-B. NMR spectra of Azido-PHA homopolymers. A. PHON₃. B. PHDN₃. and PHDDN₃.

FIGS. 46A-C. The mol ratio of repeating units in azido-PHA samples in relation to the starting fatty acid substrate ratio. A. PHOON₃. B. PHODN₃. C. PHODDN₃.

FIGS. 47A-B. Isolated yields of azido-PHA samples. A. PHOON₃ and PHODN₃. B. PHODDN₃.

FIGS. 48A-B. Molecular weight determinations of azido-PHA homo- and copolymers. A. PHON₃, PHDN₃, PHDDN₃ and PHOON₃. B. PHODN₃ and PHODDN₃.

FIG. 49. Representative FT-IR spectrum of azido-PHAs

FIG. 50. Thermal properties of PHOON₃, PHODN₃, and PHODDN₃.

FIG. 51. Example of a multivalent PHA (PHO-co-PHDN3-co-PHU) produced with E. coli LSBJ.

5. DETAILED DESCRIPTION OF THE INVENTION

An Escherichia coli-based system is provided for producing PHA polymers (also referred to herein as “PHAs”) with defined repeating unit composition. The system can be used to control repeating unit composition in PHAs. Methods for producing PHA polymers using the system are also provided. Recombinant bacteria and genetic constructs for producing the bacteria are also provided.

The system and method can be used for producing desired PHAs of virtually any repeating unit composition desired, including long-chain length (LCL), medium-chain length (MCL) and short-chain-length (SCL). Over 150 PHA monomers are known in the art, and the system and methods disclosed herein can produce virtually any desired combination of repeating unit composition, millions of different types of PHAs with different physical properties, can be produced.

In a specific embodiment, a method for producing PHAs with defined repeating unit composition is also provided. In one embodiment, the method utilizes E. coli and comprises the step of providing an E. coli chromosome in which the eliminating the fadB and fadJ genes from the β-oxidation pathway have been eliminated. In a specific embodiment, the E. coli chromosome LS5218.

The subsequent blockage in β-oxidation causes a buildup of enoyl-CoA intermediates. The method also comprises the step of converting the enoyl-CoA intermediates PHAs by an (R)-specific enoyl-CoA hydratase (PhaJ4) and PHA synthase [PhaC1 (STQK)] expressed from a plasmid DNA construct.

In another embodiment, the method can comprise the step of providing fatty acid substrates and converting fatty acid substrates to PHAs with repeating units equal in the number of carbon atoms to the fatty acid substrates. The broad substrate specificities of the PhaJ4 and PhaC1(STQK) enzymes allows for the production of homopolymers with strict control over the repeating unit composition.

In another embodiment, a modified Escherichia coli strain is provided to allow for control over repeating unit composition of poly(3-hydroxyalkanoate) (PHA) polymers. In one embodiment, E. coli LS5218 is used as the parent strain. E. coli LS5218 metabolizes short to long chain fatty acids without induction. In one embodiment, the β-oxidation pathway of E. coli LS5218 is blocked at the enoyl-CoA intermediate by gene deletion of (S)-specific enoyl-CoA hydratases, fadB and fadJ. PHAs are synthesized from enoyl-CoA intermediates in the β-oxidation pathway by an (R)-specific enoyl-CoA hydratase (PhaJ4) and PHA synthase (PhaC1(STQK)) expressed from a plasmid construct.

A repeating unit of desired (or prescribed) length in carbon atoms is selected by providing the modified E. coli with a fatty acid equal in number of carbons. Both PHA homopolymer and PHA copolymer repeating unit compositions may be controlled. In a specific embodiment, PHA homopolymer biosynthesis produces repeating units from four to twelve carbons in length. In other embodiments, PHA copolymer composition can be controlled via manipulation of different fatty acid ratios made available to the E. coli.

For clarity of disclosure, and not by way of limitation, the detailed description of the invention is divided into the subsections set forth below.

5.1 Method for Producing a Prescribed Unit Size Poly(3-hydroxyalkanoate) (PHA) Polymer and Recombinant Bacterium for Use Therein

A method is provided for producing a prescribed unit size poly(3-hydroxyalkanoate) (PHA) polymer. A recombinant bacterium for use in the method is also provided.

In one embodiment of the method, the method comprises the steps of:

-   -   providing a recombinant (i.e., genetically engineered)         bacterium, wherein the bacterium:         -   metabolizes short to long chain fatty acids without             induction, and         -   expresses an (R)-specific enoyl-CoA hydratase and a PHA             synthase;     -   feeding at least one fatty acid substrate to the recombinant         bacterium, the at least one fatty acid substrate being of equal         carbon length to the prescribed unit size of the PHA polymer to         be produced; and     -   isolating and/or purifying the PHA from the recombinant         bacterium, thereby producing the PHA polymer.

A prescribed unit size refers to the desired number of carbon atoms that are in the unit.

In one embodiment, the (R)-specific enoyl-CoA hydratase and PHA synthase have wide substrate specificities.

The PHA polymer can be a PHA homopolymer or a PHA copolymer. More than 150 PHA monomers are known in the art. PHA homopolymers or copolymers can be produced from any PHA monomer and/or combination of monomers known in the art. PHA copolymers can consist of random mixtures of repeating units of different sizes, where the composition depends upon the carbon source, the growth conditions, and the organism chosen for producing the PHAs.

In a preferred embodiment of the method, the recombinant bacterium is a recombinant E. coli. In other embodiments, other bacteria known in the art to be suitable for bacterial fermentation, such as Ralstonia eutropha, Cupriavidus necator, Alcaligenes lattus, Pseudomonas spp., (e.g., Pseudomonas putida, Pseudomonas oleovorans, Pseudomonas aeruginosa), Aeromonas cavia, Burkholderia cepacia, Bacillus subtilis, Pseudomonas sp. 61-3, Corynebacterium glutamicum and Bacillus spp. can be used.

In another embodiment of the method, the step of providing a recombinant bacterium comprises the step of constructing a plasmid comprising an (R)-specific enoyl-CoA hydratase gene and a PHA synthase gene and transforming the plasmid into the recombinant bacterium. Methods for transforming bacteria with plasmids are well known in the art.

Genetic constructs and vectors are also provided. In one embodiment, a plasmid is provided comprising an (R)-specific enoyl-CoA hydratase gene and a PHA synthase gene. In a specific embodiment, the plasmid is pBBR-C1J4SII, as described in Examples 1 and 2.

In a specific embodiment of the method, the recombinant bacterium is a recombinant E. coli and the plasmid is pBBR-C1J4SII, described in Examples 1 and 2 below. Plasmids can be constructed using methods well known in the art. To construct pBBR-C1J4SII, for example, the phaJ4 gene from Pseudomonas putida KT2440 is PCR amplified using the phaJ4 primers “phaJ4 left EcoRV” and “phaJ4 right KpnI” (Table 2), PrimeSTAR HS DNA polymerase (Takara), and an iCycler thermal cycler (Bio-Rad). The PCR product is gel-purified and cloned into the pCR-Blunt (Invitrogen) vector with T4 DNA ligase (New England Biolabs). Successful ligations are confirmed via sequencing from Genewiz. The resulting Blunt-phaJ4 vector is digested with KpnI and the excised piece phaJ4 DNA is ligated into the KpnI site of the plasmid pBBRSTQKAB, creating pBBRSTQKABJ4. Correct orientation of the phaJ4 gene on the pBBRSTQKABJ4 plasmid is confirmed by restriction enzyme digest with BamHI. pBBRSTQKABJ4 is then digested with SacII to excise the unnecessary genes phaA and phaB. The linearized DNA fragment is gel-purified and ligated together at the SacII site with T4 DNA ligase, generating pBBR-C1J4SII. pBBR-C1J4SII can be transformed into E. coli LSBJ following standard procedures and grown on LB agar plates with kanamycin. Single colonies are added to 50 ml LB cultures with kanamycin and grown for 12-16 hours in a 30° C. incubator with shaking at 250 rpm. One milliliter of the overnight culture is used to inoculate 100 ml of LB with kanamycin, a fatty acid substrate at 2 g L⁻¹, and the surfactant Brij-35 (Fisher Scientific) at 4 g L⁻¹ in a baffled flask. The flasks are incubated at 30° C. with shaking at 250 rpm for 48 hours. Cells are incubated, harvested and dried via lyophilization.

To ensure repeating unit control of PHA synthesis could be ascertained over a large range of repeating unit lengths, the (R)-specific enoyl-CoA hydratase and PHA synthase preferably both have wide substrate specificities. In one embodiment, PhaJ4 from Pseudomonas putida KT2440 can be used for converting the enoyl-CoA intermediate of β-oxidation to (R)-3-hydroxyacyl-CoA.

In another embodiment of the method, β-oxidation is blocked in the recombinant bacterium, thereby causing a buildup of enoyl-CoA intermediates.

In another embodiment of the method, at least one gene encoding an enzyme for β-oxidation is inactive or deleted from the chromosome of the recombinant bacterium. Such methods are known in the art, examples of which are described in Examples 1 and 2.

In another embodiment of the method, a plurality of genes encoding enzymes for β-oxidation is inactive or deleted from the chromosome of the recombinant bacterium.

In another embodiment of the method, the at least one gene encoding an enzyme for β-oxidation is a gene encoding an (S)-specific enoyl-CoA hydratase.

In another embodiment of the method, the at least one gene encoding an enzyme for β-oxidation is a fad gene.

In a preferred embodiment of the method, the recombinant bacterium is recombinant E. coli and an (S)-specific enoyl-CoA hydratasefadB gene and/or a fadJ gene is deleted from the chromosome of the recombinant E. coli.

Deletions of enzymes involved in fatty acid degradation and expression of PHA synthesis enzymes with broad substrate specificities allow for controlled PHA synthesis utilizing fatty acid substrates equal in length to the desired repeating unit. The fatty acid starting materials enter the β-oxidation pathway but are stopped at the enoyl-CoA intermediate (FIG. 1). Instead of proceeding through β-oxidation and degrading, the enoyl-CoA intermediate accumulates due to elimination of the (S)-specific enoyl-CoA hydratasefadB and its homologue fadJ (formerly yfcX). Deletion of these genes from E. coli eliminates the ability of the organism to grow on fatty acids. Since the β-oxidation pathway is stopped prior to degradation of the fatty acid, any fatty acids that enter the pathway are converted to PHA substrates equal in length as the starting fatty acid.

In a specific embodiment, the enoyl-CoA intermediates are converted to (R)-3-hydroxyacyl-CoA by the (R)-specific enoyl-CoA hydratase (PhaJ4) and polymerized by a PHA synthase [PhaC1(STQK)].

In another embodiment of the method, the recombinant bacterium constitutively expresses a positive regulator of an operon, wherein the operon promotes uptake of short chain fatty acids for β-oxidation.

In another embodiment of the method, the recombinant bacterium is a recombinant E. coli and the recombinant E. coli constitutively expresses a positive regulator of the ato operon, thereby promoting uptake of short chain fatty acids for β-oxidation. In a specific embodiment, the recombinant E. coli constitutively expresses AtoC.

In another embodiment of the method, the recombinant bacterium is a recombinant E. coli, the (R)-specific enoyl-CoA hydratase is PhaJ4 and the PHA synthase is PhaC1(STQK).

Any suitable fatty acid known in the art can be used as a substrate. Suitable fatty acids preferably do not have substituents that prevent it from entering or being taken up by the recombinant bacterium. Two or more (i.e., a plurality) of fatty acid substrates can be co-fed to the bacteria at varying ratios for incorporation into a PHA polymer or copolymer with a desired or controlled repeating unit composition. In a specific embodiment, the fatty acid substrates are 3-12 carbons in length. In another embodiment, the fatty acid substrates are 3-16 carbons in length.

Thus, in another embodiment of the method, at least two different types of fatty acid substrates of desired length are fed to the recombinant bacteria in a desired ratio, thereby producing a PHA polymer or PHA copolymer of desired (or controlled) repeating unit composition. Methods for selecting substrates of desired length in a desired ratio to produce a PHA polymer or PHA copolymer of desired (or controlled) repeating unit composition are described in Examples 1 and 2.

Methods for culturing recombinant bacteria (e.g., bacterial fermentation) to produce a bacterially produced (fermentation) product are also well known in the art.

In one embodiment of the method, PHA is isolated or purified from the recombinant bacteria, thereby producing a PHA polymer. Methods for recovering PHAs from recombinant bacteria are described herein.

In a specific embodiment, the recombinant bacteria are cultured (e.g., in batch fermentation or culture) and the PHA recovered from the cultured bacteria. Such methods are well known in the art.

Characterization and determination of physical properties of PHAs produced by the method and system disclosed herein can be performed using techniques well known in the art. Repeating unit compositions of PHAs can be determined by gas chromatography (GC) and gas chromatography-mass spectroscopy (GC-MS). The number-average molecular weight (M_(n)) and the weight-average molecular weight (M_(w)) can be determined by gel permeation chromatography (GPC). Nuclear magnetic resonance can be performed for further structural analysis Thermal analysis can be performed to determine such characteristics as decomposition temperatures (T_(d)), glass-transition temperatures (T_(g)), melting temperatures (T_(m)), and crystallization temperatures (T_(c)).

In other embodiments, growth and polymer production can also be performed using undecylenic acid and lactic acid at similar ratios. Undecylenic acid is an 11-carbon fatty acid with a terminal alkene, allowing the insertion of a chemically reactive handle into the polymers produced. PHA polymers containing repeating units with terminal alkenes have been chemically modified to expand the number of applications for drug delivery and shape-memory polymers.

5.2 System for Producing a Prescribed Unit Size Poly(3-hydroxyalkanoate) (PHA) Polymer

A system is provided for producing a prescribed unit size poly(3-hydroxyalkanoate) (PHA) polymer. The system comprises the recombinant bacterium described above in Section 5.1

Thus in one embodiment of the system, the system comprises a recombinant (genetically engineered) bacterium, wherein the bacterium:

-   -   metabolizes short to long chain fatty acids without induction,         and     -   expresses an (R)-specific enoyl-CoA hydratase and a PHA synthase

In one embodiment, the (R)-specific enoyl-CoA hydratase and PHA synthase have wide substrate specificities.

A prescribed unit size refers to the desired number of carbon atoms that are in the unit.

In another embodiment of the system, the PHA polymer is a PHA homopolymer or a PHA copolymer.

In another embodiment of the system, the recombinant bacterium is a recombinant E. coli.

In another embodiment of the system, the recombinant bacterium comprises a plasmid comprising an (R)-specific enoyl-CoA hydratase gene and a PHA synthase gene.

In another embodiment of the system, the recombinant bacterium is a recombinant E. coli and the plasmid is pBBR-C1J4SII.

In another embodiment of the system, β-oxidation is blocked in the recombinant bacterium, thereby causing a buildup of enoyl-CoA intermediates.

In another embodiment of the system, at least one gene encoding an enzyme for β-oxidation is inactive or deleted from the chromosome of the recombinant bacterium.

In another embodiment of the system, the at least one gene encoding the enzymes for β-oxidation are is a fad gene.

In another embodiment of the system, a plurality of genes encoding enzymes for β-oxidation is inactive or deleted from the chromosome of the recombinant bacterium.

In another embodiment of the system, the at least one gene encoding an enzyme for β-oxidation is a gene encoding an (S)-specific enoyl-CoA hydratase.

In another embodiment of the system, the at least one gene encoding an enzyme for β-oxidation is a fad gene.

In another embodiment of the system, the recombinant bacterium is recombinant E. coli and an (S)-specific enoyl-CoA hydratase fadB gene and/or a fadJ gene is deleted from the chromosome of the recombinant E. coli.

In another embodiment of the system, the recombinant bacterium constitutively expresses a positive regulator of an operon, wherein the operon promotes uptake of short chain fatty acids for β-oxidation.

In another embodiment of the system, the recombinant bacterium is a recombinant E. coli and wherein the recombinant E. coli constitutively expresses a positive regulator of the ato operon, thereby promoting uptake of short chain fatty acids for β-oxidation.

In another embodiment of the system, the recombinant E. coli constitutively expresses AtoC.

In another embodiment of the system, the recombinant bacterium is a recombinant E. coli, the (R)-specific enoyl-CoA hydratase is PhaJ4 and the PHA synthase is PhaC1(STQK).

5.3 Poly(3-hydroxyalkanoates) (PHAs) Produced by the Method and System

Poly(3-hydroxyalkanoate) (PHA) polymers and copolymers are provided with prescribed unit size repeating units.

Poly(3-hydroxyalkanoates) (PHAs) are biodegradable plastics and carbon storage materials that are synthesized by a myriad of microorganisms. PHAs are divided into groups based on repeating unit size. PHAs with repeating units of three to five carbons in length are short-chain-length (SCL) PHAs, and PHAs with repeating units of six to fourteen carbons in length are medium-chain-length (MCL) PHAs. Differences in repeating unit composition influence the physical properties of PHAs. Both short-chain-length (SCL) PHAs and medium-chain-length (MCL) PHAs are provided.

In specific embodiments, SCL and MCL PHAs comprising fatty acids with terminal vinyl groups (alkenes and alkynes) are provided.

Gaining control over such a broad range of repeating units in PHAs, as provided by the method and system described herein, is useful for numerous applications in which PHAs can be used, particularly for the production of MCL PHAs that can then be analyzed for medical uses. Various SCL-co-MCL PHA copolymers have been tested for heart valve engineering, nerve regeneration, skin regeneration, drug delivery and more. These applications require specific physical properties that can be attained with the proper ratio of repeating units within PHAs, as described in more detail in Examples 1 and 2. The method and system disclosed herein allows for selection of repeating unit sizes and ratios aids in synthesis of PHAs with medically relevant physical properties.

5.4 Methods and Systems for Producing Prescribed Unit Size Azido-poly(3-hydroxyalkanoate) (Azido-PHA) Polymers and Copolymers

Methods and systems for producing prescribed unit size azido-poly(3-hydroxyalkanoate) (azido-PHA) polymers and copolymers are provided. The methods and systems can employ recombinant bacteria that are not native producers of PHA or lack enzymes to degrade PHA once synthesized, metabolize short to long chain fatty acids without induction, and express an (R)-specific enoyl-CoA hydratase and a PHA synthase, the (R)-specific enoyl-CoA hydratase and PHA synthase having wide substrate specificities. The recombinant bacteria are fed at least one ω-azidofatty acid substrate that is equal in carbon length to the prescribed or desired unit size of an azido-PHA polymer to be produced. The prescribed unit size azido-PHA polymer or azido-PHA co-polymer that is produced is then isolated and/or purified. Azido-PHA polymers or azido-PHA co-polymers can then be conjugated via copper-catalyzed alkyne-azide cycloaddition (CuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) reactions. These methods and systems are described in detail in Section 6.4 (Example 4).

In one embodiment, the method for producing a prescribed unit size azido-poly(3-hydroxyalkanoate) (azido-PHA) polymer comprises:

-   -   (a) providing a recombinant bacterium, wherein the bacterium:         -   i. metabolizes short to long chain fatty acids without             induction, and         -   ii. expresses an (R)-specific enoyl-CoA hydratase and a             poly(3-hydroxyalkanoate) (PHA) synthase;     -   (b) feeding to the recombinant bacterium a fatty acid substrate,         wherein the fatty acid substrate is a ω-azidofatty acid         substrate, the ω-azidofatty acid comprising a terminal azide,         and the ω-azidofatty acid being of equal carbon length to the         prescribed unit size of the azido-PHA polymer to be produced;         and     -   (c) isolating and/or purifying the azido-PHA polymer from the         recombinant bacterium, thereby producing the prescribed unit         size azido-PHA polymer.

In another embodiment of the method, the bacterium is not a native or natural producer of PHA or lacks enzymes to degrade PHA once synthesized.

In another embodiment of the method, the PHA polymer is a PHA homopolymer, a PHA copolymer or a PHA-co-polylactic acid (PLA) polymer.

In another embodiment, the method comprises, after (c),

-   -   conjugating the azido-PHA polymer via a copper-catalyzed         alkyne-azide cycloaddition (CuAAC) reaction or a strain-promoted         azide-alkyne cycloaddition reaction (SPAAC) with alkynes,     -   thereby producing a conjugated PHA polymer or an orthogonally         conjugated PHA co-polymer.

In another embodiment of the method, (b) further comprises feeding at least one of 3-hydroxybutyrate, 3-hydroxyoctanoate, or 3-hydroxydodecanoate monomer to the recombinant bacterium, and wherein after (c), a conjugated PHA polymer or an orthogonally conjugated PHA co-polymer is produced.

In another embodiment of the method, the recombinant bacterium is a recombinant E. coli.

In another embodiment of the method, at least two different types of fatty acid substrate of desired length are fed to the recombinant bacteria in a desired ratio, and wherein at least one of the two different types of fatty acid substrate comprises a terminal azide moiety, thereby producing an azidoPHA homopolymer, azido-PHA copolymer of desired or controlled repeating unit composition.

A system is also provided for producing a prescribed unit size azido-poly(3-hydroxyalkanoate) (azido-PHA) polymer comprising a recombinant bacterium, wherein the bacterium:

-   -   a. metabolizes short to long chain fatty acids without         induction, and     -   b. expresses an (R)-specific enoyl-CoA hydratase and a PHA         synthase.

In one embodiment of the system, the bacterium is not a native or natural producer of PHA or lacks enzymes to degrade PHA once synthesized.

In another embodiment of the system, the recombinant bacterium is a recombinant E. coli.

In another embodiment of the system, the recombinant bacterium comprises a plasmid comprising an (R)-specific enoyl-CoA hydratase gene and a PHA synthase gene.

Production of PHA_(scl/mcl)-co-PHX and Multivalent Copolymers

Copolymers are provided in the form PHA_(scl/mcl)co-PHX as well as multivalent PHA_(scl/mcl)co-PHX-co-PHX. To broaden the physical properties and application spectrum of PHAs, copolymers can be produced of the form PHA_(scl/mer)co-PHX as well as multivalent PHA_(scl/mcl)co-PHX-co-PHX, where “PHA_(scl/mcl)” represents either a base monomer encompassed by SCL fatty acids (C3-05) or MCL fatty acids (C6-C14), and “PHX” represents MCL fatty acids of variable chain lengths containing either a terminal alkene (X=U_(ene)), alkyne (X=U_(yne)) or azide (X=N₃) moiety. These functional MCL fatty acids enable designed PHA copolymers to be orthogonally conjugated via thiol-ene click reactions (alkenes/alkynes) and standard CuAAC or SPAAC reactions (alkynes/azides) as described in Section 6.4 (Example 4).

FIG. 51 shows an example of a multivalent PHA (PHO-co-PHDN3-co-PHU) produced with E. coli LSBJ using the methods disclosed herein.

5.5 Uses of PHA Polymers

Targeted Drug Delivery

Methods for targeted drug delivery are provided. After bacterial synthesis of PHA polymers, functionalized targeting agents can be added by click chemistry to improve selectivity for an assortment of pathologies as described in as described in Section 6.4 (Example 4) (FIG. 38). The functionalized PHA polymers are formulated into nanoparticles via miniemulsion and/or nano-precipitation, and characterized by dynamic light scattering and atomic force microscopy (AFM).

Tissue Engineering

Small oligopeptides can be used to induce cellular proliferation on tissue engineering scaffolds. The RGD signal peptide has been shown to important cellular recognition and to aid in cellular attachment. To provide a tissue engineering scaffold for guided cellular attachment, click-ready PHAs can be functionalized as follows: A dibenzocyclooctyne-Arg-Gly-Asp (DBCO-RGD) peptide is synthesized as previously described (Wang, X., Gobbo, P., Suchy, M., Workentin, M. S., and Hudson, R. H. E. 2014). Peptide-decorated gold nanoparticles via strain-promoted azide alkyne cycloaddition and post assembly deprotection (RSC Advances. 4. 43087-43091). This is conjugated via a SPAAC to the PHA-azide polymers as described in 6.4, Example 4 (for BCN—OH and conjugation with azido-PHAs).

Imaging

Clickable PHAs can be used as imaging agents. Dibenzylcyclooctyne-Fluor 568 (DBCO-5) can be attached to the azido-PHA nanoparticles as shown in FIG. 39. Other compounds employed may include fluorescent imaging reagents containing cyclooctyne-variants such as OCT (Agard N J, Prescher J A, Bertozzi C R (2004) A strain-promoted [3+2] azide-alkyne cycloaddition for covalent modification of biomolecules in living systems. J Am Chem Soc 126:15046-15047), ALO (Agard N J, Baskin J M, Prescher J A, Lo A, Bertozzi C R (2006) A comparative study of bioorthogonal reactions with azides. ACS Chem Biol 1:644-648), MOFO (Agard N J, Baskin J M, Prescher J A, Lo A, Bertozzi C R (2006) A comparative study of bioorthogonal reactions with azides. ACS Chem Biol 1:644-648), DIFO (Baskin J M, et al. (2007) Copper-free click chemistry for dynamic in vivo imaging. Proc Natl Acad Sci USA 104:16793-16797), BARAC (Rapid Cu-Free Click Chemistry with Readily Synthesized Biarylazacyclooctynones John C. Jewett, Ellen M. Sletten, and Carolyn R. Bertozzi, Journal of the American Chemical Society 2010 132 (11), 3688-3690), DIBO (Ning X.; Guo J.; Wolfert M. A.; Boons G.-J. Visualizing Metabolically-Labeled Glycoconjugates of Living Cells by Copper-Free and Fast Huisgen Cycloadditions, Angew. Chem., Int. Ed. 2008, 47, 2253-2255), DIMAC (Sletten E M, Bertozzi C R (2008) A hydrophilic azacyclooctyne for Cu-free click chemistry. Org Lett 10:3097-3099), DIFBO (Sletten E M, Nakamura H, Jewett J C, Bertozzi C R, Difluorobenzocyclooctyne: synthesis, reactivity, and stabilization by beta-cyclodextrin. J Am Chem Soc. 2010; 132(33):11799-805) and DIBAC (M. F. Debets, S. S. van Berkel, S. Schoffelen, F. P. J. T. Rutjes, J. C. M. van Hest, F. L. van Delft, Aza-dibenzocyclooctynes for fast and efficient enzyme PEGylation via copper-free (3+2) cycloaddition. Chem. Commun. 2010, 46, 97-99), BCN (Dommerholt, J.; Schmidt, S.; Temming, R.; Hendriks, L. J. a; Rutjes, F. P. J. T.; Van Hest, J. C. M.; Lefeber, D. J.; Friedl, P.; Van Delft, F. L. Readily Accessible Bicyclononynes for Bioorthogonal Labeling and Three-Dimensional Imaging of Living Cells. Angew. Chemie—Int. Ed. 2010, 49, 9422-9425).

Biotinylated PHAs

Biotin derivatives can be attached to PHAs using methods known in the art. Section 6.5, Example 5 describes the attachment of dibenzocylooctyne-PEG4-biotin (DBCO-PEG4-biotin) to an azido PHA to produce azido-PHA-DBCO-PEG4 biotin. Azido-PHA-DBCO-PEG4 biotin nanoparticles can be used as a nucleic acid carrier for transformation of cells.

The following examples are offered by way of illustration and not by way of limitation.

6. EXAMPLES

6.1 Example 1: Precise Control of Repeating Unit Composition in Biodegradable Poly[(R)-3-hydroxyalkanoate] Polymers Synthesized by Escherichia coli

The composition of medium-chain-length (MCL) poly(3-hydroxyalkanoate) (PHA) biopolymers is normally an uncontrollable random mixture of repeating units with differing side chain lengths. Attempts to generate MCL PHA homopolymers and gain control of the repeating unit composition have been published in native PHA-producing organisms but have limited ranges for the different sizes of repeating units that can be synthesized. This example describes an Escherichia coli-based system that exhibits control over repeating unit composition of both MCL and short-chain-length (SCL) PHAs, covering an unprecedented range of repeating units. The fadB and fadJ genes from the β-oxidation pathway were eliminated from the chromosome of E. coli LS5218. The subsequent blockage in β-oxidation caused a buildup of enoyl-CoA intermediates, which were converted to PHAs by an (R)-specific enoyl-CoA hydratase (PhaJ4) and PHA synthase (PhaC1(STQK)) expressed from a plasmid DNA construct. Fatty acid substrates were converted to PHAs with repeating units equal in the number of carbon atoms to the fatty acid substrate. The broad substrate specificities of the PhaJ4 and PhaC1(STQK) enzymes allowed for the production of homopolymers with strict control over the repeating unit composition from substrates of four to twelve carbons in length. Polymers were purified and analyzed by GC, GC-MS, and NMR for structural composition and by DSC (thermogravimetric analysis), TGA (thermogravimetric analysis), and GPC for thermal and physical characteristics. This study marks the development of the first single biological system to achieve consistent repeating unit control over such a broad range of repeating units in PHAs.

Introduction

Poly(3-hydroxyalkanoates) (PHAs) are biodegradable plastics and carbon storage materials that are synthesized by a myriad of microorganisms (1). PHAs are divided into groups based on repeating unit size. PHAs with repeating units of three to five carbons in length are short-chain-length (SCL) PHAs, and PHAs with repeating units of six to fourteen carbons in length are medium-chain-length (MCL) PHAs. Differences in repeating unit composition influence the physical properties of PHAs (2,3). Production of SCL PHA homopolymers such as poly-3-hydroxybutyrate (PHB) is well studied (4,5). Previous attempts to control MCL PHA synthesis in native and recombinant PHA-producing organisms, however, have resulted in narrow ranges of repeating unit control (6-9). Typically, control is limited to only a couple of repeating units within one organism, (6,7,9) or control is lost once the number of carbons in the repeating unit exceeds seven (8). In this example, Escherichia coli LS5218 has been engineered to develop a new PHA-production system capable of synthesizing PHAs with repeating unit compositions based solely on the carbon chain length of fatty acid substrates fed to the strain. PHA synthesis control is demonstrated for repeating units from four and up to twelve carbons long, spanning both SCL and MCL PHAs.

Gaining control over such a broad range of repeating units in PHAs is useful for a number of applications for which PHAs can be used, particularly for the production of MCL PHAs for medical uses. Various SCL-co-MCL PHA copolymers have been tested for heart valve engineering, nerve regeneration, skin regeneration, drug delivery and more (10). These applications require specific physical properties that may be attained with the proper ratio of repeating units within PHAs. The system disclosed herein allows for selection of repeating unit sizes and ratios aids in synthesis of PHAs with medically relevant physical properties.

In this example, PHAs with homogenous repeating units were synthesized to demonstrate control over repeating unit size selection. This work demonstrates an engineered microbial system capable of controlling the molecular composition of PHA polymers over a broad range of repeating unit sizes including MCL and SCL PHAs.

Deletions of enzymes involved in fatty acid degradation and expression of PHA synthesis enzymes with broad substrate specificities allow for controlled PHA synthesis utilizing fatty acid substrates equal in length to the desired repeating unit. The fatty acid starting materials enter the β-oxidation pathway but are stopped at the enoyl-CoA intermediate (FIG. 1). Instead of proceeding through β-oxidation and degrading, the enoyl-CoA intermediate accumulates due to elimination of the (S)-specific enoyl-CoA hydratase fadB and its homologue fadJ (formerly yfcX). Deletion of these genes from E. coli eliminates the ability of the organism to grow on fatty acids (11). Numerous studies have been carried out that utilize β-oxidation mutations to promote PHA synthesis in vivo (12-16). However, no work has been presented with mutations of both fadB and fadJ for the production of PHAs comprised of specific repeating units as displayed here. Since the β-oxidation pathway is stopped prior to degradation of the fatty acid, any fatty acids that enter the pathway are converted to PHA substrates equal in length as the starting fatty acid. The enoyl-CoA intermediates were converted to (R)-3-hydroxyacyl-CoA by the (R)-specific enoyl-CoA hydratase (PhaJ4) and polymerized by a PHA synthase [PhaC1(STQK)].

E. coli LS5218 was chosen as the parental strain for generating this system because of its abilities to uptake and degrade a broad range of fatty acids. Normally, the E. coli genes responsible for uptake and degradation of medium to long-chain fatty acids via the β-oxidation pathway require induction by fatty acids of at least fourteen carbons in length (17). E. coli LS5218 has a mutated fadR gene, which leads to constitutive expression of the fad genes encoding the enzymes for β-oxidation (12,18). E. coli LS5218 also has an atoC(Con) mutation allowing for constitutive expression of AtoC, a positive regulator of the ato operon, which is responsible for uptake of short chain fatty acids for β-oxidation (19,20). These mutations allow LS5218 to readily metabolize short to very long chain fatty acids without induction.

To ensure repeating unit control of PHA synthesis could be ascertained over a large range of repeating unit lengths, the (R)-specific enoyl-CoA hydratase and PHA synthase must both have wide substrate specificities. Therefore, PhaJ4 from Pseudomonas putida KT2440 was selected for converting the enoyl-CoA intermediate of β-oxidation to (R)-3-hydroxyacyl-CoA. PhaJ4 from P. putida KT2440 has 78% amino acid identity to PhaJ4 from Pseudomonas aeruginosa, which was reported to have relatively high activity for enoyl-CoA substrates ranging in size from four to twelve carbons (21,22). (R)-specific enoyl-CoA hydratases have been used numerous times to synthesize PHAs (14,21-25), but none of the studies demonstrated the ability to control PHA repeating unit composition as the system generated here.

The PHA synthase used in this work was PhaC1(STQK), an engineered PHA synthase derived from the native Pseudomonas sp. 61-3 PHA synthase (26). The wild type PHA synthase was unique in its ability to incorporate both SCL and MCL units in PHAs (27) and was engineered to increase its ability to incorporate SCL units. This PHA synthase has been used successfully to produce PHAs containing repeat units of four to twelve carbons in length (28,29), making it a suitable partner for the PhaJ4 enzyme for synthesizing a wide range of PHAs with varying side chain lengths.

The unique E. coli repeating unit control system developed in this study allows for unprecedented control of PHA repeating unit composition within one bacterial strain. The desired repeating unit(s) can be selected for incorporation into PHAs simply by selecting the fatty acid substrate of equal carbon length. This system has several advantages over previous attempts at producing PHA homopolymers. The E. coli strain made in this study is capable of utilizing a wide range of fatty acids, without induction, and converting them to PHA polymers with uniform repeating unit composition. Another advantage is that E. coli is not a native producer of PHAs and lacks the enzymes to degrade the PHAs once synthesized. This study demonstrates, for the first time within one biological system, PHA production with the ability to tightly control repeating unit composition for repeating units from four and up to twelve carbons.

Example 2 describes the next step in this work, which is the production of specified short-chain-length and medium-chain-length single repeating units in poly[(R)-3-hydroxyalkanoates] synthesized in E. coli using fatty acid substrates.

Example 3 describes synthesis of SCL-MCL PHA, MCL PHA-co-PLA, or SCL-MCL PHA-co-PLA co-polymers from fatty acid substrates.

Materials and Methods

Media and Cultivation

Strains and plasmids used in this example are listed in Table 1. All E. coli strains were grown on Lennox Broth (LB; composition per liter: 10 g tryptone, 5 g yeast extract, and 5 g sodium chloride) at 30° C. and 250 rpm on a rotary shaker. The following antibiotics were added as appropriate to growth media: kanamycin at 50 μg ml⁻¹, ampicillin at 100 μg ml⁻¹, and chloramphenicol at 30 μg ml⁻¹. Any further additions to the media or culture conditions were as described below.

TABLE 1 Strains and plasmids Strain of Plasmid Relevant Characteristics Source or Reference Strains E. coli LS5218 fadR601, atoC(Con) Coli Stock Center E. coli JM109 recA1 endA1 gyrA96 thi-1 hsdR17 (r_(K) ⁻m_(K) ⁺) Promega supE44 relA1 l-lac [F′ proAB lacI^(q) ZDM15] E. coli LSBJ E. coli LS5218 fadB::Cm, fadJ (—) This Example Plasmids pKD46 λ Red recombinase expression plasmid; Datsenko & expresses exo, β, and γ genes from λ phage; Wanner, 2000 P_(araB) promoter; araC; Amp^(r); temperature sensitive replicon pKD3 Chloramphenicol acetyltransferase flanked Datsenko & by FLP recombinase recognition targets, Wanner, 2000 Amp^(r), Cm^(r) pKD13 Neomycin phosphotransferase flanked by Datsenko & FLP recombinase recognition targets, Km^(r), Wanner, 2000 Amp^(r) pCP20 FLP recombinase expression plasmid, Datsenko & Amp^(r), temperature sensitive replicon Wanner, 2000 pBBRSTQKAB pBBR1MCS-2 derivative, phaC1 (STQK), Nomura et al., 2004 phaAB pBBRSTQKABJ4 pBBRSTQKAB derivative, phaJ4 This Example pBBR-C1J4SII SacII digest of pBBRSTQKABJ4 (to This Example remove phaAB), phaJ4, phaC1 (STQK) pCR-Blunt Cloning vector for blunt PCR-cloning, Invitrogen ccdB-lacZfusion, Km^(r) Blunt-J4 pCR-Blunt derivative, phaJ4 This Example

Gene Knockouts in E. coli LS5218

The method for chromosomal gene inactivation in E. coli developed by Datsenko and Wanner (30) was used to inactivate the fadB and fadJ genes in E. coli LS5218. Primers for the antibiotic genes within plasmids pKD13 and pKD3 contained ends homologous to fadJ and fadB, respectively (Table 2). Knockout cassettes were amplified from appropriate plasmid templates using PrimeSTAR HS DNA polymerase (Takara) and an iCycler thermal cycler (Bio-Rad) following manufacturers' recommendations. E. coli LS5218 carrying the pKD46 plasmid was grown in LB with 0.3% (w/v) L-arabinose to induce expression of the λ Red system. Cells that had expressed the λ Red system from the pKD46 plasmid and grown to an OD₆₀₀ of ˜0.6 were made electrocompetent and transformed with PCR knockout cassettes via an ECM 399 electroporator (BTX) at 1500 volts following the manufacturer's protocols for E. coli. Transformants were selected on LB-agar plates with the appropriate antibiotic. Elimination of the genes from the chromosome was confirmed by PCR (FIGS. 2A-B). The temperature-sensitive plasmid pKD46 was expelled from the cells by growth at 37° C.

The gene encoding fadJ was eliminated from E. coli LS5218 first by insertion of the gene expressing kanamycin resistance into the fadJ gene on the chromosome. This insert was removed by expression of FLP recombinase from the pCP20 plasmid. Successful removal was confirmed by PCR and loss of kanamycin resistance. The temperature-sensitive plasmid pCP20 was expelled from the cells by growth at 37° C. After fadJ was eliminated, fadB was eliminated by insertion of the chloramphenicol acetyltransferase cassette from pKD3. All deletions were confirmed by PCR (FIGS. 2A-B).

TABLE 2 Oligonucleotide primers Primers Sequence^(a) (5′ to 3′) Gene Knockout pKD13.F.fadJ ATGGAAATGACATCAGCGTTTACCCTTAATGTTCG TCTGGACAACATTGCGTGTAGGCTGGAGCTGCTTC (SEQ ID NO: 1) pKD13.R.fadJ TTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAAC TTTCCCCACGCGCGCATTCCGGGGATCCGTCGACC (SEQ ID NO: 2) pKD3.F.fadB ATGCTTTACAAAGGCGACACCCTGTACCTTGACTG GCTGGAAGATGGCATGTGTAGGCTGGAGCTGCTTC (SEQ ID NO: 3) pKD3.R.fadB TTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTG GCTCAACCGGAGGATCATATGAATATCCTCCTTAG (SEQ ID NO: 4) fadJ.F.check GGTTTAGTTACCGCCTGTGC (SEQ ID NO: 5) fadJ.R.check AGCGCGGATTCATATAGCTT (SEQ ID NO: 6) fadB.F.check GCGAGTCCGTTCTTGTAAGG (SEQ ID NO: 7) fadB.R.check CGAATTGCATCGACAATGAC (SEQ ID NO: 8) PHA-Expression phaJ4 F KpnI ATTGGTACCCAACTGACAACCCGGAGAGT (SEQ ID NO: 9) phaJ4 R EcoRV ATTGATATCCTGGCAGTTTACGCGAGTG (SEQ ID NO: 10) ^(a)Underlined sequences are homologous to genes to be knocked out and sequences in bold are restriction enzyme sites

Construction of pBBR-C1J4SII Vector for PHA Production

The phaJ4 gene from Pseudomonas putida KT2440 (22,31) was PCR amplified using the phaJ4 primers “phaJ4 left EcoRV” and “phaJ4 right KpnI” (Table 2), PrimeSTAR HS DNA polymerase (Takara), and an iCycler thermal cycler (Bio-Rad). The PCR product was gel-purified and cloned into the pCR-Blunt (Invitrogen) vector with T4 DNA ligase (New England Biolabs). Successful ligations were confirmed via sequencing from Genewiz. The resulting Blunt-phaJ4 vector was digested with KpnI and the excised piece phaJ4 DNA was ligated into the KpnI site of the plasmid pBBRSTQKAB (28), creating pBBRSTQKABJ4. Correct orientation of the phaJ4 gene on the pBBRSTQKABJ4 plasmid was confirmed by restriction enzyme digest with BamHI. pBBRSTQKABJ4 was then digested with SacII to excise the unnecessary genes phaA and phaB. The linearized DNA fragment was gel-purified and ligated together at the SacII site with T4 DNA ligase, generating pBBR-C1J4SII. All restriction enzymes were purchased from New England Biolabs. All cloning transformations and plasmid preparations were performed in E. coli JM109.

PHA Production in E. coli LSBJ

pBBR-C1J4SII was transformed into E. coli LSBJ following standard procedures (32) and grown on LB agar plates with kanamycin. Single colonies were added to 50 ml LB cultures with kanamycin and grown for 12-16 hours in a 30° C. incubator with shaking at 250 rpm. One milliliter of the overnight culture was used to inoculate 100 ml of LB with kanamycin, a fatty acid substrate at 2 g L⁻¹, and the surfactant Brij-35 (Fisher Scientific) at 4 g L⁻¹ in 500 ml baffled flasks. The flasks were incubated at 30° C. with shaking at 250 rpm for 48 hours. The fatty acids used were sodium butyrate (Alfa Aesar), sodium octanoate (Sigma), decanoic acid (Alfa Aesar), 10-undecenoic acid (SAFC), or lauric acid (Acros Organics).

Flasks to produce PHB from sodium butyrate also contained 6 g L⁻¹ monobasic potassium phosphate and 13.24 g L⁻¹ dibasic potassium phosphate. Half of the total sodium butyrate for PHB production was added after cells grew for eight hours and the second half at twenty-four hours. After incubation was complete, the cells were harvested by centrifugation at 3,716×g for 15 min. Pelleted cells were washed with 70% (v/v) ethanol, pelleted again, and then washed with Nanopure water (Barnstead). The cells were then dried via lyophilization.

Purification of PHAs

The purification of PHAs produced in E. coli LSBJ was based on the work published by Jiang et al. (33). Dried cells were suspended in methanol at 22 ml methanol per gram of dried cells and mixed by stirring at room temperature for five minutes. Cells were collected by centrifugation, washed with Nanopure water (Barnstead), and dried via lyophilization. PHAs were then extracted from the cells in a soxhlet extractor with 120 ml of refluxing acetone for five hours. The extract was concentrated in a rotovap and cast in a glass Petri dish.

Gas Chromatography (GC) and Gas Chromatography-mass Spectroscopy (GC-MS) Analysis of PHAs Produced

Repeating unit compositions of PHAs were determined by GC and GC-MS. For GC analysis, either dried cells (15-20 mg) or purified polymers (5-10 mg) were dissolved in 2 ml chloroform and 2 ml sulfuric acid:methanol (15:85) and heated at 100° C. for 140 minutes. After cooling the samples to room temperature, 1 ml of water was added to each sample. Samples were mixed by vortexing, and the aqueous and organic layers were separated by centrifugation at 863×g for 3 min. The organic layer containing the 3-hydroxymethyl esters was passed through a 0.45 μm polytetrafluoroethylene (PTFE) syringe filter (Restek). A total of 500 μl of the filtered sample was mixed with 500 μl of caprylic acid (1 g L⁻¹) in chloroform in a GC vial. Samples were then loaded by 1 μl split injection at 280° C. into a GC 2010 Gas Chromatograph with a flame ionization detector at 310° C. (Shimadzu). Samples were separated in a 30 m Rtx®-5 column (Restek, 0.25 mm id and 0.25 μmd_(f)). The heating profile for the column oven was as follows: 100° C. initial temperature, ramp to 280° C. at 8° C. min⁻¹, hold for 2 min, ramp to 310° C. at 20° C. min⁻¹, and hold for 2 min Isolated polymers and fatty acid feedstocks were also analyzed by GC-MS, which was performed as previously described (34). Methyl/ethyl esters corresponding to PHA repeating units were determined based on retention times of known standards and resulting mass spectra when available.

Gel Permeation Chromatography (GPC) Analysis of PHAs Produced

The number-average molecular weight (M_(n)) and the weight-average molecular weight (M_(w)) were determined by GPC using a LC-20AD Liquid Chromatograph equipped with a SIL-20A auto-sampler and RID-10A refractive index detector (Shimadzu). Purified PHA samples were dissolved in chloroform at 0.7 mg/ml and passed through a 0.45 μm PTFE syringe filter. 50 μl of each sample were passed through a styrenedivinylbenzene (SDV) 8×300 mm column with 5 μm porosity (Polymer Standards Service). The column oven was maintained at 40° C. Chloroform was the mobile phase with a flow rate of 1 ml min⁻¹. Molecular weights were determined based on polystyrene standards as determined previously (4).

Nuclear Magnetic Resonance of PHAs Produced

For further structural analysis, 10-15 mg of purified PHAs from E. coli LSBJ were dissolved in 1 ml of deuterated chloroform and analyzed by gradient-enhanced-HSQC-DEPT NMR spectroscopy. All spectra were acquired at 30° C. with a Bruker AVANCE 600 spectrometer (600 MHz ¹H frequency) equipped with a 5 mm triple resonance z-gradient probe. Data were acquired and processed in TOPSPIN v1.3 from Bruker BioSpin. The ge-HSQC-DEPT experiment had spectral widths of 4800 Hz and 25000 Hz with 2k×256 data points collected for ¹H and ¹³C spectra, respectively.

Thermal Analysis of PHAs Produced

Purified polymers were kept at room temperature for at least one week prior to thermal analysis experiments. The decomposition temperatures (T_(d)) of the PHAs produced were determined by thermogravimetric analysis (TGA) using a TGA Q5000IR (TA Instruments). Approximately 10 mg of a sample were heated to 500° C. at a rate of 20° C. min⁻¹. The T_(d) was taken at the temperature when sample degradation was initiated. The PHA samples were subsequently checked for glass-transition temperatures (T_(g)), melting temperatures (T_(m)), and crystallization temperatures (T_(c)) by differential scanning calorimetry (DSC) with a DSC Q200 (TA Instruments). Approximately 10 mg of polymer were heated to 200° C. at 10° C. min⁻¹ in a nitrogen atmosphere. Samples were then cooled to −80° C. at a rate of 5° C. min⁻¹ and subsequently reheated to 200° C. at 10° C. min⁻¹. The T_(g) and T_(m) were recorded during the second heating of the sample. The T_(c) was taken during the cooling of the sample from 200° C. to −80° C.

Results

PHA Production and Composition in E. coli LSBJ

To develop a bacterial strain capable of producing PHA polymers with defined repeating unit composition, E. coli LSBJ was made from E. coli LS5218 by eliminating the (S)-specific enoyl-CoA hydratase fadB and its homologue fadJ on the chromosome (FIGS. 2A-B).

FIG. 2A shows a map of the Escherichia coli genome at the genes fadB and fadJ before and after removal of the genes. The knockout of fadB was carried out by the insertion of a chloramphenicol acetyltransferase (cat). The knockout of fadJ was carried out by insertion of a neomycin phosphotransferase and subsequent removal, leaving behind a short scar sequence. The numbers next to each map represents approximate PCR product sizes with the “check” primers in Table 2. Half arrows indicate approximate ligation sites for primers a (fadB.F.check), b fadB.R.check), x (fadJ.F.check), and y (fadJ.R.check).

FIG. 2B shows agarose electrophoresis gel of PCR products of fadB and fadJ regions of E. coli LS5218 and E. coli LSBJ. Lane 1, 1 kb ladder (New England Biolabs); Lane 2, PCR from E. coli LS5218 using fadB.F.check and fadB.R.check primers; Lanes 3 and 4, PCR from E. coli LSBJ using fadB.F.check and fadB.R.check primers; Lane 5, PCR from E. coli LS5218 using fadJ.F.check and fadJ.R.check primers; and Lanes 6 and 7, PCR from E. coli LSBJ using fadJ.F.check and fadJ.R.check primers.

E. coli LSBJ then produced PHAs with defined repeating unit composition from fatty acids while expressing PhaJ4 and PhaC1(STQK). The fatty acids used to produce the defined polymers were the SCL fatty acid sodium butyrate (C4) and the MCL fatty acids, sodium octanoate (C8), decanoic acid (C10), 10-undecenoic acid (C11:1, A10), and dodecanoic acid (C12). The repeating units of the PHAs produced were representative of the fatty acid substrate in number of carbons (Table 3). Gas chromatography (GC) initially showed each of the PHAs to be comprised entirely of repeating units equal in number of carbons as the starting fatty acid. For example, samples grown in the presence of sodium octanoate were comprised of 3-hydroxyoctanoate repeating units and no other MCL repeating units.

The only potential contaminants seen in the initial GC experiments of samples produced from the medium chain fatty acids were seen as peaks with retention times that matched 3-hydroxybutyrate (3HB). To confirm the absence or presence of 3HB in the PHAs not produced from sodium butyrate, polymer samples were analyzed by HSQC-DEPT NMR (FIGS. 3-6).

FIG. 3 shows ¹H-¹³C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxyoctanoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. Purified polymer was dissolved in deuterated chloroform and checked for contaminating repeating units, specifically poly(3-hydroxybutyrate) (PHB). CH and CH₃ carbons are shown in black, and CH₂ signals are shown in gray. In addition to comparison of the spectra with previous works of NMR on PHAs, the HSQC-DEPT NMR experiments were performed to confirm the absence of 3-hydroxybutyrate (3HB) repeating units in the various MCL-PHAs. 3HB within the polymer would be differentiated from the desired MCL repeating units by looking for the chiral carbon (the third carbon) and the methyl carbon's (the fourth carbon) signals. The chiral carbon of 3HB would be slightly shielded compared to that of the MCL-PHAs (67-68 ppm vs. 70-71 ppm) in the ¹³C portion of the spectra. The methyl carbon would give a black signal in the area of 1.30 ppm for ¹H and 19-20 ppm for ¹³C. The signals in the poly(3-hydroxyoctanoate) sample that do not correspond to signals found in other MCL-PHA samples with fully saturated side chains are believed to be residual fatty acid substrate (octanoate) that was not fully removed from extracted polymer. This sample does not show detectable PHB.

FIG. 4 shows ¹H-¹³C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxydecanoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. Purified polymer was dissolved in deuterated chloroform and checked for contaminating repeating units, specifically poly(3-hydroxybutyrate). CH and CH₃ carbons are shown in black, and CH₂ signals are shown in gray. In addition to comparison of the spectra with previous works of NMR on PHAs, the HSQC-DEPT NMR experiments were performed to confirm the absence of 3-hydroxybutyrate (3HB) repeating units in the various MCL-PHAs. 3HB within the polymer would be differentiated from the desired MCL repeating units by looking for the chiral carbon (the third carbon) and the methyl carbon's (the fourth carbon) signals. The chiral carbon of 3HB would be slightly shielded compared to that of the MCL-PHAs (67-68 ppm vs. 70-71 ppm) in the ¹³C portion of the spectra. The methyl carbon would give a black signal in the area of 1.30 ppm for ¹H and 19-20 ppm for ¹³C. This sample does not show detectable PHB.

FIG. 5 shows ¹H-¹³C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxy-10-undecenoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. Purified polymer was dissolved in deuterated chloroform and checked for contaminating repeating units, specifically poly(3-hydroxybutyrate). CH and CH₃ carbons are shown in black, and CH₂ signals are shown in gray. In addition to comparison of the spectra with previous works of NMR on PHAs, the HSQC-DEPT NMR experiments were performed to confirm the absence of 3-hydroxybutyrate (3HB) repeating units in the various MCL-PHAs. 3HB within the polymer would be differentiated from the desired MCL repeating units by looking for the chiral carbon (the third carbon) and the methyl carbon's (the fourth carbon) signals. The chiral carbon of 3HB would be slightly shielded compared to that of the MCL-PHAs (67-68 ppm vs. 70-71 ppm) in the ¹³C portion of the spectra. The methyl carbon would give a black signal in the area of 1.30 ppm for ¹H and 19-20 ppm for ¹³C. The fatty acid substrates were found to have detectable amounts of different fatty acids in GC-MS experiments. This may have been the cause for detectable amounts of repeating units (GC-MS) in PHAs not equal in size to the starting substrate. The fatty acid substrate for production of poly(3-hydroxy-10-undecenoate) (PHUn), 10-undecenoic acid, was listed as 95% pure by the manufacturer (SAFC). The contaminating materials likely contain fully saturated fatty acids. These fully saturated fatty acids would also be incorporated into PHAs synthesized by this system. These potential contaminants can be seen in the ¹H spectrum of the PHUn sample at ˜0.8 ppm. This is the same chemical shift seen for the terminal methyl groups of the polymers containing fully saturated side chains in the repeating units (FIGS. 3, 4 and 6). This sample does not show detectable PHB.

FIG. 6 shows ¹H-¹³C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxydodecanoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. Purified polymer was dissolved in deuterated chloroform and checked for contaminating repeating units, specifically poly(3-hydroxybutyrate). CH and CH₃ carbons are shown in black, and CH₂ signals are shown in gray. In addition to comparison of the spectra with previous works of NMR on PHAs, the HSQC-DEPT NMR experiments were performed to confirm the absence of 3-hydroxybutyrate (3HB) repeating units in the various MCL-PHAs. 3HB within the polymer would be differentiated from the desired MCL repeating units by looking for the chiral carbon (the third carbon) and the methyl carbon's (the fourth carbon) signals. The chiral carbon of 3HB would be slightly shielded compared to that of the MCL-PHAs (67-68 ppm vs. 70-71 ppm) in the ¹³C portion of the spectra. The methyl carbon would give a black signal in the area of 1.30 ppm for ¹H and 19-20 ppm for ¹³C. This sample does not show detectable PHB.

Chemical shifts were compared to previous works utilizing NMR to analyze PHAs (28,35,36). Chemical shifts corresponding to the 3HB repeating unit were not observed in the NMR experiments.

Extracted PHAs were also analyzed by gas chromatography-mass spectroscopy (GC-MS), and MCL repeating units not equal in carbon length to the starting fatty acid were found. However, these minor fractions were in amounts lower than the GC detection limit and only qualitatively detected with GC-MS (Table 3). The carbon sources for the PHAs (i.e. fatty acids) were also analyzed by GC-MS, and the substrates were also found to have detectable amounts of contaminating fatty acids. Fully saturated fatty acids were present in large enough quantities in the 10-undecenoic acid substrate that the subsequent incorporation of the contaminants in the PHAs could be detected in HSQC-DEPT NMR of the poly(3-hydroxy-10-undecenoate) (PHUn) sample (FIG. 5).

PHB was also synthesized using this system. Sodium butyrate was added to the cell media to generate the SCL PHA. Table 3 shows that PHB was accumulated at 7.81% of the cell dry weight in E. coli LSBJ. The PHB synthesized was not in large enough quantities for further purification and testing. After successful synthesis of PHB in this system, it is inferred that all repeating units can be synthesized and controlled from four and up to twelve carbons in length.

TABLE 3 PHA yield and composition from single fatty acid substrates PHA Composition (mol %)** PHA CDW PHA 3HB 3HHx 3HO 3HN 3HD 3HUn 3HDD 3HTD Substrate (g L⁻¹) (wt %) (C4) (C6) (C8) (C9) (C10) (C11) (C12) (C13) Sodium 1.29 ± 0.03  7.81 ± 0.21 100 0 0 0 0 0 0 0 Butyrate Sodium 0.86 ± 0.02 47.3 ± 5.0 0 0 ≤100*   0 trace 0 trace 0 Octanoate Decanoic 1.03 ± 0.01 25.7 ± 0.9 0 0 trace 0 ≤100*   0 trace 0 Acid 10- 1.07 ± 0.03 41.0 ± 8.0 0 0 0 0 0 ≤100*   0 trace Undecenoic Acid Dodecanoic 1.03 ± 0.05 28.6 ± 8.0 0 0 0 0 trace 0 ≤100*   0 Acid *Values shown determined by GC. GC-MS data showed qualitatively detectable amounts (labeled “trace”) of other repeating units. **3HB, 3-hydroxybutyrate; 3HHx, 3-hydroxyhexanoate; 3HO, 3-hydroxyoctanoate; 3HN, 3-hydroxynonanoate; 3HD, 3-hydroxydecanoate; 3HUn, 3-hydroxy-10-undecenoate; 3HDD, 3-hydroxydodecanoate; 3HTD, 3-hydroxytridecanoate. All values are averages of triplicate experiments plus or minus the standard deviation about those averages.

Physical and Thermal Characterization of MCL PHAs Produced in E. coli LSBJ

To examine the physical properties of the PHA polymers produced, the weight average molecular weights (M_(w)), number average molecular weights (M_(n)), and polydispersities (M_(w)/M_(n)) were determined by gel permeation chromatography (GPC) (Table 4). The decomposition temperatures (T_(d)) for each of the MCL PHAs produced were determined by thermogravimetric analysis (TGA); and the glass-transition temperatures (T_(g)), melting temperatures (T_(m)), and crystallization temperatures (T_(c)) were determined by differential scanning calorimetry (DSC). These results are shown in Table 4. The DSC and TGA scans are displayed in FIGS. 7A-B.

TABLE 4 PHA physical properties Molecular Weight PHA* M_(w) (kDa) M_(n) (kDa) M_(w)/M_(n) T_(g) (° C.) T_(m) (° C.) T_(c) (° C.) T_(d) (° C.) PHO 224 104 2.2 −42.1 ND ND 236.8 PHD 270 111 2.4 −39.8 57.7 23.1 245.3 PHUn 233 76 3.1 −61.6 ND ND 244.4 PHDD 65 40 1.6 ND 71.9 41.3 242.2 *PHO, poly(3-hydroxyoctanoate); PHD, poly(3-hydroxydecanoate); PHUn, poly(3-hydroxy-10-undecenoate); PHDD, poly(3-hydroxydodecanoate), ND, not detected.

FIGS. 7A-B show thermal analysis of purified medium-chain-length poly(3-hydroxyalkanoates). Differential scanning calorimetry scans are shown in (A), and thermogravimetric analysis results are shown in (B) for purified poly(3-hydroxyoctanoate) (PHO), poly(3-hydroxydecanoate) (PHD), poly(3-hydroxy-10-undecenoate) (PHun), and poly(3-hydroxydodecanoate) (PHDD).

Discussion

The E. coli LSBJ strain developed here in combination with the PHA production plasmid pBBR-C1J4SII allows strict control of repeating unit composition of PHAs for both MCL and SCL PHAs. The desired PHAs can be synthesized based simply on selecting a fatty acid substrate of equal carbon length. As shown in Table 3, PHAs were synthesized in sizes ranging from four to twelve carbons. Because of the native enoyl-CoA hydratase knockouts, the resulting PHAs were near homopolymers based on GC, GC-MS, and NMR analysis. GC-MS analysis found detectable amounts of repeating units different in carbon lengths than the starting fatty acid substrate, but this is likely due to impurities of the substrates and media (Table 3 and FIG. 5).

E. coli LSBJ is particularly useful for MCL PHA production from fatty acids. The parent strain, E. coli LS5218, is known to have increased uptake and metabolism for a broad range of fatty acids without the need for transcriptional induction of the appropriate fad genes by FadR (17,18). The PhaJ4 and PhaC1(STQK) introduced to synthesize the PHAs have high activity towards MCL substrates. This allowed for the strain to generate MCL PHAs at 25% cell dry weight or greater (Table 3).

Although the system created here was designed with MCL PHA repeating unit control in mind, control can also be extended to SCL PHAs. The advantages of E. coli LSBJ extend to SCL sized fatty acids, and thus PHB was synthesized from sodium butyrate. This was expected as the uptake for shorter fatty acids does not need to be induced in this strain (18), and the PhaJ4 and PhaC1(STQK) enzymes selected for PHA synthesis have reported or inferred activity for four carbon substrates (21,22,26). The PHB yield was much lower than the MCL PHAs. This could be because of enzyme substrate specificity favoring MCL substrates.

This system offers the broadest range of repeating unit control of PHAs in a single organism. Other work has been reported to control repeating unit composition in Pseudomonas putida KT2442 (8). The polymer yields here were lower compared to the Pseudomonas system developed by Wang et al., but the Pseudomonas system was unable to synthesize homopolymers with repeating units greater than seven carbons. The E. coli LSBJ system developed in this study was shown to maintain repeating unit control up to and including repeating units of twelve carbons. The size limits for repeating unit control of this system have not been determined as of this writing.

The thermal properties of the MCL PHAs produced here have similarities and differences when compared to other works that have produced MCL PHA homopolymers (8,9). Poly(3-hydroxyoctanoate) (PHO), for example, showed a T_(g) of −42.1° C., which is lower than other works producing polymers of ≥98 mol % PHO (8,9). No melting temperature was seen here for PHO, which is not the case for reports of the other studies with ≥98 mol % PHO polymers (8,9). However, the other studies report very different melting temperatures. The study by Wang et al. reports a melting temperature of 66.06° C., but a melting endothermic peak cannot be seen on the DSC curve (8). The study by Rai et al. reported a range of melting temperatures between 39° C. and 50° C., but DSC curves are not presented (9). DSC curves for this work can be found in the FIGS. 7A-B. There are many differences in these works for producing, purifying, and characterizing the polymers, which may account for the lack of consistency in reported thermal properties of PHO homopolymers.

Another MCL PHA homopolymer that was produced in this example was poly(3-hydroxydecanoate) (PHD) (7). Both the work presented here and the work by Liu et al. report glass transitions between −37° C. and −40° C. The previously produced PHD homopolymer had a reported melting temperature of 72.2° C. compared to the melting temperature of 57.7° C. for the PHD produced in this work. Once again, differences in synthesis, purification, and characterization methods could play a role in the differences in reported thermal properties.

Melting, crystallization, and glass transition temperatures can be predicted for MCL PHA homopolymers based on repeating unit size in PHAs. The T_(g) for these polymers may decrease with increasing side chain length as has been suggested by another work developing MCL PHA homopolymers (8). If this is true, the glass-transition temperature for poly(3-hydroxydodecanoate) (PHDD) may be near or below the −80° C. minimum of the DSC scans performed. There was no definitive trend in the degradation temperatures of all the polymers tested (Table 4 and FIGS. 7A-B). The thermal properties and trends of polymers here did not coincide with the properties of the polymers reported by Wang et al. (8). However, this could be the result of differing polymer purification, polymer storage conditions, molecular weights, and/or experimental conditions for DSC and TGA.

The molecular weights varied for the MCL PHAs, and the polydispersities determined by GPC ranged from 1.6 to 3.1 (Table 4). The molecular weights (M_(n)) were varied with PHD and PHO having noticeably larger molecular weights than PHUn and PHDD. The PHO molecular weight (M_(n)) and polydispersity are comparable to previous work with this polymer (9). The PHD molecular weight (M_(n)) is more than twice as small as a previous work producing PHD homopolymer, and the polydispersity is greater in this example (7). The PHUn and PHDD homopolymers have not been synthesized bacterially prior to this work.

Comparison of polymer molecular weights and polydispersities from this example to other works is difficult due to the differences in bacterial production. Other works have relied on native Pseudomonas PHA synthesis enzymes. The polymers in this example were made in E. coli expressing an enzyme with naturally broad substrate specificity (PhaJ4) and an enzyme with engineered broad substrate specificity [PhaC1(STQK)]. The substrate specificities of the PHA synthesis enzymes, particularly the PHA synthase, could have a significant effect on the molecular weights of the polymers. Some in vitro characterization of the substrate specificity of the PhaC1(STQK) synthase used in this example is available but limited to substrates with four to ten carbons (37). Because the relationship of substrate specificity to molecular weight of the polymer produced by PHA synthase enzymes is not completely defined, there is not sufficient information to draw conclusions as to why there are differences in the molecular weights of the PHA polymers produced in this example. However, the differences in the substrate specificity of engineered and native PHA synthases may be responsible for the differences in the molecular weights of polymers.

The strain E. coli LSBJ was developed to allow for repeating unit control in PHA biosynthesis. Knockouts of the genes fadB and fadJ created a dam in the β-oxidation pathway (FIG. 1). Instead of being degraded, fatty acid substrates were converted to PHAs by PhaJ4 and PhaC1 (STQK). This allowed for control of repeating unit composition simply by selecting the fatty acid that matches the desired repeating unit in carbon length. This strain successfully demonstrated repeating unit control in PHAs with repeating units of four, eight, ten, eleven, and twelve carbons. This represents the first bacterial strain to achieve such diversity in repeating unit control of PHAs.

Example 1 References

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Y., and Doi, Y.: Enrichment of     specific monomer in medium-chain-length poly(3-hydroxyalkanoates) by     amplification of fadD and fadE genes in recombinant Escherichia     coli. Enzyme Microb. Technol., 33, 62-70 (2003). -   17. DiRusso, C. C., Heimert, T. L., and Metzger, A. K.:     Characterization of FadR, a global transcriptional regulator of     fatty acid metabolism in Escherichia coli. Interaction with the fadB     promoter is prevented by long chain fatty acyl coenzyme As. J. Biol.     Chem., 267, 8685-8691 (1992). -   18. Spratt, S. K., Ginsburgh, C. L., and Nunn, W. D.: Isolation and     genetic characterization of Escherichia coli mutants defective in     propionate metabolism. J. Bacteriol., 146, 1166-1169 (1981). -   19. Pauli, G. and Overath, P.: ato Operon: a highly inducible system     for acetoacetate and butyrate degradation in Escherichia coli.     Eur. J. Biochem., 29, 553-562 (1972). -   20. Jenkins, L. S. and Nunn, W. D.: Genetic and molecular     characterization of the genes involved in short-chain fatty acid     degradation in Escherichia coli: The ato system. J. Bacteriol., 169,     42-52 (1987). -   21. Tsuge, T., Taguchi, K., Taguchi, S., and Doi, Y.: Molecular     characterization and properties of (R)-specific enoyl-CoA hydratases     from Pseudomonas aeruginosa: Metabolic tools for synthesis of     polyhydroxyalkanoates via fatty acid β-oxidation. Int. J. Biol.     Macromol., 31, 195-205 (2003). -   22. Sato, S., Kanazawa, H., and Tsuge, T.: Expression and     characterization of (R)-specific enoyl coenzyme A hydratases making     a channeling route to polyhydroxyalkanoate biosynthesis in     Pseudomonas putida. Appl. Microbiol. Biotechnol., 90, 951-959     (2011). -   23. Fukui, T., Yokomizo, S., Kobayashi, G., and Doi, Y.:     Co-expression of polyhydroxyalkanoate synthase and (R)-enoyl-CoA     hydratase genes of Aeromonas caviae establishes copolyester     biosynthesis pathway in Escherichia coli. FEMS Microbiol. Lett.,     170, 69-75 (1999). -   24. Tsuge, T., Fukui, T., Matsusaki, H., Taguchi, S., Kobayashi, G.,     Ishizaki, A., and Doi, Y.: Molecular cloning of two (R)-specific     enoyl-CoA hydratase genes from Pseudomonas aeruginosa and their use     for polyhydroxyalkanoate synthesis. FEMS Microbiol. Lett., 184,     193-198 (2000). -   25. Budde, C. F., Riedel, S. L., Willis, L. B., Rha, C., and     Sinskey, A. J.: Production of     poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by     engineered Ralstonia eutropha strains. Appl. Environ. Microbiol.,     77, 2847-2854 (2011). -   26. Takase, K., Taguchi, S., and Doi, Y.: Enhanced synthesis of     poly(3-hydroxybutyrate) in recombinant Escherichia coli by means of     error-prone PCR mutagenesis, saturation mutagenesis, and in vitro     recombination of the type II polyhydroxyalkanoate synthase gene. J.     Biochem., 133, 139-145 (2003). -   27. Matsusaki, H., Manji, S., Taguchi, K., Kato, M., Fukui, T., and     Doi, Y.: Cloning and molecular analysis of the     poly(3-hydroxybutyrate) and     poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) biosynthesis genes in     Pseudomonas sp. strain 61-3. J. Bacteriol., 180, 6459-6467 (1998). -   28. Nomura, C. T., Tanaka, T., Gan, Z., Kuwabara, K., Abe, H.,     Takase, K., Taguchi, K., and Doi, Y.: Effective enhancement of     short-chain-length-Medium-chain-length polyhydroxyalkanoate     copolymer production by coexpression of genetically engineered     3-ketoacyl-acyl-carrier-protein synthase III (fabH) and     polyhydroxyalkanoate synthesis genes. Biomacromolecules, 5,     1457-1464 (2004). -   29. Nomura, C. T., Tanaka, T., Eguen, T. E., Appah, A. S.,     Matsumoto, K., Taguchi, S., Ortiz, C. L., and Doi, Y.: FabG mediates     polyhydroxyalkanoate production from both related and nonrelated     carbon sources in recombinant Escherichia coli LS5218. Biotechnol.     Frog., 24, 342-351 (2008). -   30. Datsenko, K. A. and Wanner, B. L.: One-step inactivation of     chromosomal genes in Escherichia coli K-12 using PCR products. Proc.     Natl. Acad. Sci. U.S.A., 97, 6640-6645 (2000). -   31. Wang, Q. and Nomura, C. T.: Monitoring differences in gene     expression levels and polyhydroxyalkanoate (PHA) production in     Pseudomonas putida KT2440 grown on different carbon sources. J.     Biosci. Bioeng., 110, 653-659 (2010). -   32. Sambrook, J. J. and Russell, D. W.: Molecular cloning: a     laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring     Harbor, N.Y. (2001). -   33. Jiang, X., Ramsay, J. A., and Ramsay, B. A.: Acetone extraction     of mcl-PHA from Pseudomonas putida KT2440. J. Microbiol. Methods,     67, 212-219 (2006). -   34. Arai, Y., Nakashita, H., Suzuki, Y., Kobayashi, Y., Shimizu, T.,     Yasuda, M., Doi, Y., and Yamaguchi, I.: Synthesis of a novel class     of polyhydroxyalkanoates in Arabidopsis peroxisomes, and their use     in monitoring short-chain-length intermediates of β-oxidation. Plant     Cell Physiol., 43, 555-562 (2002). -   35. Matsusaki, H., Abe, H., Taguchi, K., Fukui, T., and Doi, Y.:     Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by     recombinant bacteria expressing the PHA synthase gene phaC1 from     Pseudomonas sp. 61-3. Appl. Microbiol. Biotechnol., 53, 401-409     (2000). -   36. Sparks, J. and Scholz, C.: Synthesis and characterization of a     cationic Poly((3-hydroxyalkanoate). Biomacromolecules, 9, 2091-2096     (2008). -   37. Matsumoto, K., Takase, K., Aoki, E., Doi, Y., and Taguchi, S.:     Synergistic effects of Glu130Asp substitution in the type II     polyhydroxyalkanoate (PHA) synthase: Enhancement of PHA production     and alteration of polymer molecular weight. Biomacromolecules, 6,     99-104 (2005).

6.2 Example 2: Production of Specified Short-chain-length and Medium-chain-length Single Repeating Units in poly[(R)-3-hydroxyalkanoate] Copolymers Synthesized in Escherichia coli Using Fatty Acid Substrates

This example describes extension of the work described in Example 1. The production of specified short-chain-length and medium-chain-length single repeating units in poly[(R)-3-hydroxyalkanoates] were synthesized in E. coli using fatty acid substrates. This example also provides an overview and summary of the results obtained in Example 1.

Introduction

Poly(3-hydroxyalkanoates) (PHAs) are biodegradable plastics and carbon storage materials that are synthesized by a myriad of microorganisms. PHAs are divided into groups based on repeating unit size. PHAs with repeating units of three to five carbons in length are short-chain-length (SCL) PHAs, and PHAs with repeating units of six to fourteen carbons in length are medium-chain-length (MCL) PHAs. Differences in repeating unit composition influence the physical properties of PHAs. SCL PHA homopolymers such as poly-3-hydroxybutyrate (PHB) have been previously produced, but methods to control the repeating unit composition of the MCL PHAs have been limited. Previous attempts to control MCL PHA synthesis in native and recombinant PHA-producing organisms have resulted in narrow ranges of repeating unit control (6-9). Typically, control is limited to only a couple of repeating units within one organism (6,7,9) or control is lost once the number of carbons in the repeating unit exceeds seven (8).

In this example, Escherichia coli LS5218 was engineered to develop a PHA-production system capable of synthesizing PHAs with repeating unit compositions based solely on the carbon chain length of fatty acid substrates fed to the strain. PHA synthesis control is demonstrated for repeating units from four and up to twelve carbons long, spanning both SCL and MCL PHAs.

Gaining control over such a broad range of repeating units in PHAs is useful for a number of applications for which PHAs are being considered, particularly for the production of MCL PHAs being analyzed for medical uses. Various SCL-co-MCL PHA copolymers have been tested for heart valve engineering, nerve regeneration, skin regeneration, drug delivery and more (10). These applications require specific physical properties that can be attained with the proper ratio of repeating units within PHAs. A system that allows for selection of repeating unit sizes and ratios aids in synthesis of PHAs with medically relevant physical properties.

This example demonstrates an engineered microbial system capable of controlling the molecular composition of PHA polymers over a broad range of repeating unit sizes including MCL and SCL PHAs. PHAs with homogenous repeating units were synthesized and control over repeating unit size selection was demonstrated.

Deletions of enzymes involved in fatty acid degradation and expression of PHA synthesis enzymes with broad substrate specificities allow for controlled PHA synthesis utilizing fatty acid substrates equal in length to the desired repeating unit. The fatty acid starting materials enter the β-oxidation pathway but are stopped at the enoyl-CoA intermediate (FIG. 1). Instead of proceeding through β-oxidation and degrading, the enoyl-CoA intermediate accumulates due to elimination of the (S)-specific enoyl-CoA hydratase fadB and its homologue fadJ (formerly yfcX). Deletion of these genes from E. coli eliminates the ability of the organism to grow on fatty acids (11). Numerous studies have been carried out that utilize β-oxidation mutations to promote PHA synthesis in vivo (12-16). However, no work has been presented with mutations of both fadB and fadJ for the production of PHAs comprised of specific repeating units as displayed here. Since the β-oxidation pathway is stopped prior to degradation of the fatty acid, any fatty acids that enter the pathway are converted to PHA substrates equal in length as the starting fatty acid. The enoyl-CoA intermediates were converted to (R)-3-hydroxyacyl-CoA by the (R)-specific enoyl-CoA hydratase (PhaJ4) and polymerized by a PHA synthase [PhaC1(STQK)].

E. coli LS5218 was chosen as the parental strain for generating this system because of its abilities to uptake and degrade a broad range of fatty acids. Normally, the E. coli genes responsible for uptake and degradation of medium to long-chain fatty acids via the β-oxidation pathway require induction by fatty acids of at least fourteen carbons in length (17). E. coli LS5218 has a mutated fadR gene, which leads to constitutive expression of the fad genes encoding the enzymes for β-oxidation (12,18). E. coli LS5218 also has an atoC(Con) mutation allowing for constitutive expression of AtoC, a positive regulator of the ato operon, which is responsible for uptake of short chain fatty acids for β-oxidation (19,20). These mutations allow LS5218 to readily metabolize short to very long chain fatty acids without induction.

To ensure repeating unit control of PHA synthesis could be ascertained over a large range of repeating unit lengths, the (R)-specific enoyl-CoA hydratase and PHA synthase preferably have wide substrate specificities. Therefore, PhaJ4 from Pseudomonas putida KT2440 was selected for converting the enoyl-CoA intermediate of β-oxidation to (R)-3-hydroxyacyl-CoA. PhaJ4 from P. putida KT2440 has 78% amino acid identity to PhaJ4 from Pseudomonas aeruginosa, which was reported to have relatively high activity for enoyl-CoA substrates ranging in size from four to twelve carbons (21,22). (R)-specific enoyl-CoA hydratases have been used numerous times to synthesize PHAs (14, 21-25),but none of the studies demonstrated the ability to control PHA repeating unit composition as the system generated here.

The PHA synthase used in this work was PhaC1(STQK), an engineered PHA synthase derived from the native Pseudomonas sp. 61-3 PHA synthase (26). The wild type PHA synthase was unique in its ability to incorporate both SCL and MCL units in PHAs (27) and was engineered to increase its ability to incorporate SCL units. This PHA synthase has been used successfully to produce PHAs containing repeat units of four to twelve carbons in length (28,29), making it a suitable partner for the PhaJ4 enzyme for synthesizing a wide range of PHAs with varying side chain lengths.

The unique E. coli repeating unit control system developed in this study allows for unprecedented control of PHA repeating unit composition within one bacterial strain. The desired repeating unit(s) can be selected for incorporation into PHAs simply by selecting the fatty acid substrate of equal carbon length. This system has several advantages over previous attempts at producing PHA homopolymers. The E. coli strain made in this study is capable of utilizing a wide range of fatty acids, without induction, and converting them to PHA polymers with uniform repeating unit composition. Another advantage is that E. coli is not a native producer of PHAs and lacks the enzymes to degrade the PHAs once synthesized. This study demonstrates, for the first time within one biological system, PHA production with the ability to tightly control repeating unit composition for repeating units from four and up to twelve carbons.

Background

In 2010, 310 million tons of plastics were generated within the United States alone (30). The vast majority of these materials are made from non-renewable fossil fuels and are neither biodegradable nor recovered for recycling at a high rate (8.2% in 2010)(30). Biodegradable plastics such as PHAs offer the potential for relief from the numerous environmental problems associated with the production and disposal of this class of material (1,2,31,32). PHAs are produced in a myriad of microorganisms (1), exhibit great structural diversity in terms of the chemical compositions of the possible repeating units (1), and undergo facile degradation in numerous settings (33-35). In addition to their use as replacements for petroleum-based plastics for bulk commodity uses, PHAs are under consideration for several more specialized applications, including the production of enantiomerically pure materials for use in the synthesis of other valuable molecules (36) and numerous medical applications using PHAs as substrates (10,37-39). As the number of applications and the demand for biorenewable plastics grow and become more specialized, it becomes increasingly critical that methods be established that permit greater control over the physical properties of such biologically synthesized plastics (including PHAs).

The physical properties of PHAs are greatly influenced by the repeating unit composition of the polymer. Each repeating unit of a natively-produced PHA polymer can typically vary in size from four to fourteen carbons. PHA copolymers can consist of random mixtures of repeating units of different sizes, where the composition depends upon the carbon source, the growth conditions, and the organism chosen for producing the PHAs (1,2,10). Based upon the size of the repeating unit, PHAs are generally referred to as either SCL or MCL when the repeat unit contains either three to five carbon atoms or six to fourteen carbon atoms, respectively. The physical properties of PHA polymers comprised of various assortments of these repeating units can continuously span the range from those for SCL to MCL PHAs depending upon the mole ratio composition (2,40). Since the repeating unit composition of PHAs greatly influences the overall material properties of these polymers, one of the critical challenges facing the use of PHAs in applications is establishment of the ability to consistently and reproducibly produce PHA polymers or copolymers with prescribed repeating unit compositions.

There have been multiple reports to the effect that PHAs can be produced with varying compositions and that suggest ways to control for these differing repeating unit combinations and content (40-43). For example, in contrast to the common in vivo bacterial system described here, in vitro polymerization using differing amounts of lactones has been utilized to control repeating unit composition in PHA copolymers (40). These chemical syntheses products, however, have lower molecular weights and were not enantiometrically pure, which limits the biodegradability of the polymers and complicates characterization (40). Incorporation of both (R)-3-hydroxyhexanoate (3HHx) and lactic acid (LA) has been achieved at different levels by mutating specific amino acids in PHA synthases and represents important work for furthering the understanding of how PHA synthases incorporate different repeating units in PHAs (41,42).

Recently, a recombinant E. coli system was developed that produced poly(3-hydroxypropionate-co-4-hydroxybutyrate) from 1,3-propanediol and 1,4-butanediol (43). The repeating unit compositions were controlled via substrate ratio variation, but the study was limited to two repeating unit types. Additionally, there are reports showing bacterial systems that produce PHA polymers with defined compositions of PHAs with only one repeating unit (4-8). These have potential use in generating copolymers with controlled repeating unit compositions, but these systems would provide control that extends only to narrow ranges of repeating units and would thus have limited potential for controlled copolymer biosynthesis. In this report, we describe a system that can incorporate the broadest array of repeating units to date (spanning SCL and MCL PHAs) and produce PHA copolymers with precisely defined mol % compositions of specific repeating units.

Example 1 describes the development of Escherichia coli LSBJ to control the repeating unit composition of PHAs over a broad range of repeating unit sizes (44). E. coli LSBJ was derived from E. coli LS5218, whose mutations in the regulation of fatty acid uptake and degradation genes allow constitutive expression of genes responsible for fatty acid utilization in E. coli (12,18,20). It was shown that the repeating unit size could be selected between four to twelve carbons by simply using a fatty acid substrate with an equal number of carbons (FIG. 1). This was accomplished in part by way of removal of the gene for the (S)-specific enoyl-CoA hydratase (fadB) and its homologue (fadJ), thereby eliminating the ability of the bacteria to oxidize fatty acids beyond enoyl-CoA (11,15). PHAs were then synthesized from the enoyl-CoA intermediate of β-oxidation by the transgenically expressed (R)-specific enoyl-CoA hydratase (PhaJ4) and PHA synthase (PhaC1(STQK)), each of which contain broad substrate specificities (21,22,26).

With the demonstration that this system can dictate the synthesis of a large range of PHA repeating units individually, the next step in the present example was to characterize E. coli LSBJ as a vehicle for the production of PHA copolymers with controlled repeating unit compositions. The present example demonstrates the ability of E. coli LSBJ to produce PHA copolymers with specific, prescribed, repeating unit compositions. Two or more (i.e., a plurality) of fatty acid substrates can be co-fed to the bacteria at varying ratios for incorporation into a PHA copolymer with a desired or controlled repeating unit composition. In this example, two different types of fatty acid substrate were co-fed to the bacteria at varying ratios for incorporation into the PHA copolymer. The PHA polymers produced in this study allowed not only for the calibration of the repeating unit compositions in PHAs but also for the selection of specific sets of physical properties within the range of the copolymers that were produced.

Materials and Methods

Media and Cultivation

A list of strains and plasmids used in this study is shown in Table 1. All E. coli strains were grown on Lennox Broth purchased from Difco (LB; composition per liter: 10 g tryptone, 5 g yeast extract, and 5 g sodium chloride) at 30° C. and 250 rpm on a rotary shaker. The following antibiotics were added as appropriate to growth media: kanamycin at 50 μg ml⁻¹, ampicillin at 100 μg ml⁻¹, and chloramphenicol at 30 μg ml⁻¹. The fatty acids dodecanoic acid (Acros Organics), decanoic acid (Alfa Aesar), sodium octanoate (Sigma-Aldrich), and sodium butyrate (Alfa Aesar) were used as substrates for PHA biosynthesis. Monobasic and dibasic potassium phosphates (BDH) were added to flasks containing sodium butyrate at a concentration of 6.0 g L⁻¹ and 13.24 g L⁻¹, respectively as a buffer. To aid dissolution of fatty acids, the surfactant Brij-35 (Fisher Scientific) was added to flasks that contained only MCL-sized fatty acid substrates (that is, dodecanoic acid, decanoic acid, and sodium octanoate) at 4.0 g ACS reagent/HPLC-grade methanol, acetone, and chloroform were used in sample pretreatment and extraction of polymers from bacterial cells. Any further additions to the media or culture conditions were as described below.

Gene Knockouts in E. coli LS5218

The method for chromosomal gene inactivation in E. coli developed by Datsenko and Wanner (45) was used to inactivate the fadB and fadJ genes in E. coli LS5218. Primers for the antibiotic genes within plasmids pKD13 and pKD3 contained ends homologous to fadJ and fadB, respectively Table 2. Knockout cassettes were amplified from appropriate plasmid templates using PrimeSTAR HS DNA polymerase (Takara) and an iCycler thermal cycler (Bio-Rad) following manufacturers' recommendations. E. coli LS5218 carrying the pKD46 plasmid was grown in LB with 0.3% (w/v) L-arabinose to induce expression of the λ Red system. Cells that had expressed the λ Red system from the pKD46 plasmid and grown to an OD₆₀₀ of ˜0.6 were made electrocompetent and transformed with PCR knockout cassettes via an ECM 399 electroporator (BTX) at 1500 volts following the manufacturer's protocols for E. coli. Transformants were selected on LB-agar plates with the appropriate antibiotic. Elimination of the genes from the chromosome was confirmed by PCR. The temperature-sensitive plasmid pKD46 was expelled from the cells by growth at 37° C.

The gene encoding fadJ was eliminated from E. coli LS5218 first by insertion of the gene expressing kanamycin resistance into the fadJ gene on the chromosome. This insert was removed by expression of FLP recombinase from the pCP20 plasmid. Successful removal was confirmed by PCR and loss of kanamycin resistance. The temperature-sensitive plasmid pCP20 was expelled from the cells by growth at 37° C. After fadJ was eliminated, fadB was eliminated by insertion of the chloramphenicol acetyltransferase cassette from pKD3. All deletions were confirmed by PCR.

Construction of pBBR-C1J4SII Vector for PHA Production

The phaJ4 gene from Pseudomonas putida KT2440 (22,46) was PCR amplified using the phaJ4 primers “phaJ4 F EcoRV” and “phaJ4 R KpnI” (Table 2), PrimeSTAR HS DNA polymerase (Takara), and an iCycler thermal cycler (Bio-Rad). The PCR product was gel-purified and cloned into the pCR-Blunt (Invitrogen) vector with T4 DNA ligase (New England Biolabs). Successful ligations were confirmed via sequencing from Genewiz. The resulting Blunt-phaJ4 vector was digested with KpnI and the excised piece phaJ4 DNA was ligated into the KpnI site of the plasmid pBBRSTQKAB (28), creating pBBRSTQKABJ4. Correct orientation of the phaJ4 gene on the pBBRSTQKABJ4 plasmid was confirmed by restriction enzyme digest with BamHI. pBBRSTQKABJ4 was then digested with SacII to excise the unnecessary genes phaA and phaB. The linearized DNA fragment was gel-purified and ligated together at the SacII site with T4 DNA ligase, generating pBBR-C1J4SII. All restriction enzymes were purchased from New England Biolabs. All cloning transformations and plasmid preparations were performed in E. coli JM109.

PHA Production in E. coli LSBJ

E. coli LSBJ was made chemically competent, transformed with the pBBR-C1J4SII plasmid following standard procedures (47), and grown on LB kanamycin plates at 37° C. Single colonies were used to inoculate 50 mL of LB with kanamycin, and these cultures were grown at 30° C. in an incubating orbital shaker at 250 rpm for 12-16 hours. Aliquots (1 mL) of these 50 mL LSBJ-C1J4SII cultures were used to inoculate 100 mL of growth media in 500 mL baffled shake flasks. The PHA-production media contained LB, kanamycin, fatty acid substrates (to 2 g L⁻¹ for total fatty acids), and either Brij-35 or potassium phosphates as outlined above. The PHA-production media was incubated at 30° C. in an orbital shaker at 250 rpm for 48 hours. After incubation was complete, cells were harvested by centrifugation at 3,716×g for 15 min Pelleted cells were resuspended in 70% (v/v) ethanol to remove residual fatty acids, and the cells were collected again by centrifugation. Finally, the cells were washed with Nanopure filtered water (Barnstead) to remove residual ethanol, and frozen at −80° C., and dried via lyophilization.

PHA Extraction

Extraction of polymer from dried cells was based on previously published work (48). After removing appropriate dried cell fractions for gas chromatography analysis, remaining cells were treated with methanol prior to extraction. Approximately 22 mL of methanol were added to dried biomass per gram of dried biomass, and the mixture was incubated at room temperature with stirring for 5 min. The biomass was collected by centrifugation, resuspended in Nanopure filtered water, and dried via lyophilization. Polymer was then removed from the cells via soxhlet extraction with 120 mL of either acetone (for samples generated from single MCL substrates only) or chloroform. The chloroform was refluxed for approximately 5 hours after the first siphoning event. The extract was concentrated in a rotary evaporator and placed in a glass Petri dish, allowing the solvent to evaporate overnight. PHA films were then kept at room temperature pending further analysis.

Gas Chromatography (GC) and Gas Chromatography-mass Spectroscopy (GC-MS) Analysis of PHAs Produced

Repeating unit compositions of PHAs were determined by GC and GC-MS. For GC analysis, either dried cells (15-20 mg) or purified polymers (5-10 mg) were dissolved in 2 ml chloroform and 2 ml sulfuric acid:methanol (15:85) and heated at 100° C. for 140 minutes. After cooling the samples to room temperature, 1 ml of water was added to each sample. Samples were mixed by vortexing, and the aqueous and organic layers were separated by centrifugation at 485×g for 3 min. The chloroform layer containing the 3-hydroxymethyl esters was passed through a 0.45 μm polytetrafluoroethylene (PTFE) syringe filter (Restek). A total of 500 μl of the filtered sample was mixed with 500 μl of caprylic acid (1 g L⁻¹) in chloroform in a GC vial. Samples were then loaded by 1 μl split injection at 280° C. into a GC 2010 Gas Chromatograph with a flame ionization detector at 310° C. (Shimadzu). Samples were separated in a 30 m Rtx®-5 column (Restek, 0.25 mm id and 0.25 μmd_(f)). The heating profile for the column oven was as follows: 100° C. initial temperature, ramp to 280° C. at 8° C. min⁻¹, hold for 2 min, ramp to 310° C. at 20° C. min⁻¹, and hold for 2 min Isolated polymers and fatty acid feedstocks were also analyzed by GC-MS, which was performed as previously described (49). Methyl/ethyl esters corresponding to PHA repeating units were determined based on retention times of known standards and resulting mass spectra when available.

Nuclear Magnetic Resonance (NMR) of PHAs Produced

For further structural analysis, 10-20 mg of purified PHAs from E. coli LSBJ were dissolved in 1 ml of deuterated chloroform, passed through a small plug of glass wool into sample tubes, and analyzed by ¹H and/or gradient-enhanced-HSQC-DEPT NMR spectroscopy. ¹H spectra were acquired at 30° C. with a Bruker DPX 300 (300 MHz ¹H frequency) equipped with a 5 mm BBFO z-gradient probe. The recycle delay was 5.0 sec, the acquisition time was 3.4 sec with a 45° pulse width, and the experiment had spectral widths of 4800 Hz. The ge-enhanced-HSQC-DEPT spectra were acquired with a Bruker AVANCE 600 spectrometer (600 MHz ¹H frequency) equipped with a 5 mm triple resonance z-gradient probe. Data were acquired and processed in TOPSPIN v1.3 from Bruker BioSpin. The ge-HSQC-DEPT experiment had spectral widths of 4800 Hz and 25000 Hz with 2k×256 data points collected for ¹H and ¹³C spectra, respectively.

Gel Permeation Chromatography (GPC) Analysis of PHAs Produced

The number-average molecular weight (M_(n)) and the weight-average molecular weight (M_(w)) were determined by GPC using a LC-20AD Liquid Chromatograph equipped with a SIL-20A auto-sampler and RID-10A refractive index detector (Shimadzu). Purified PHA samples were dissolved in chloroform at 0.7 mg/ml and passed through a 0.45 μm PTFE syringe filter. 50 μl of each sample were injected to the GPC and passed through an 8×50 mm styrenedivinylbenvene (SDV) guard column (5 μm particles; Polymer Standards Service) and an 8×300 mm SDV analytical column (5 μm particles; mixed bed porosity; max molecular weight 1E6 Da; Polymer Standards Service product sda083005lim). The column oven was maintained at 40° C. Chloroform was the mobile phase with a flow rate of 1 ml min⁻¹. Molecular weights were determined based on polystyrene standards as determined previously (4).

Thermal Analysis of PHAs Produced

The decomposition temperatures (T_(d)) of extracted PHA polymers were determined by thermogravimetric analysis (TGA). The melting temperatures (T_(m)), crystallization temperatures (T_(c)), and glass-transition temperatures (T_(g)) of the PHA polymers were determined by differential scanning calorimetry (DSC). TGA experiments were performed on a TGA Q5000IR (TA Instruments). Approximately 10 mg of extracted polymer sample was heated under nitrogen atmosphere at 20° C. min⁻¹ to 500° C. The T_(d) was taken at the initiation of sample degradation. DSC experiments were performed on a DSC Q200 (TA Instruments). Approximately 10 mg of polymer sample were heated to 200° C. at 10° C. min⁻¹, cooled to either −40° C. or −60° C. at 5° C. min⁻¹, and then heated back to 200° C. at 10° C. min⁻¹. All DSC samples were analyzed under a nitrogen atmosphere. The T_(m) and T_(c) were taken at the bottom or top of their peaks, respectively, if present. The T_(g) was taken in the middle of the transition. Both DSC and TGA data were analyzed using TA Instruments Universal Analysis 2000 software.

Tensile Strength Analysis of PHAs Produced

Extracted PHA samples were used in tensile strength experiments using a TST350 (Linkam Scientific Instruments) to determine Young's modulus, yield strength, ultimate tensile strength, and strain to failure (elongation to break). Polymer samples (400 to 800 mg) were dissolved in 10 mL chloroform and cast in glass Petri dishes (8.6 cm diameter). The dishes were covered with aluminum foil, which had small punctures to allow for even solvent evaporation. After the solvent had been allowed to evaporate overnight, the samples were moved to a vacuum desiccator for at least 24 hours. A hydraulic bench top press (Carver, Model C) was used to cut dogbones for tensile strength testing. The mold used to cut the dogbones was a scaled down ASTM Type IV mold for plastic analysis (ASTM designation: D638-10). The mold was scaled down by a factor of 2 compared to the standard, as done similarly in previous work with another standard (50). The dimensions of the cut dogbones were 1.5 mm (gauge width), 6.25 mm (gauge length), 28.75 mm (total length), and 4.75 mm (total width). Samples were measured at the middle and both ends of the dogbones using digital calipers to determine thickness. Thicknesses on average were between 0.05 and 0.15 mm Samples were loaded onto the TST350, clamped, and pulled at a rate of 20.8 μm s⁻¹. The instrument was set with a 20 N load cell with a 0.01 N resolution, and the temperature was set to 25.0° C. The strain and corresponding stress on the samples were recorded using Linksys32 software. The Young's modulus, yield strength, ultimate tensile strength, and strain to failure (elongation to break) were determined from the observed stress-strain curves. The Young's modulus was taken as the slope of the initial linear portion of the stress-strain curve. The yield strength was determined using a 2% x-intercept offset of the line equation determined for the Young's modulus. The offset yield strength was then identified as the point where the 2% offset line crossed the stress-strain curve.

Results

Creation of E. coli LSBJ and Initial PHA Biosynthesis

To develop a bacterial strain capable of producing PHA polymers with defined repeating unit composition, E. coli LSBJ was made from E. coli LS5218 by eliminating the (S)-specific enoyl-CoA hydratase fadB and its homologue fadJ on the chromosome (FIGS. 2A-B). E. coli LSBJ then produced PHAs with defined repeating unit composition from fatty acids by expressing PhaJ4 and PhaC1(STQK). The fatty acids used to produce the defined polymers were the SCL fatty acid sodium butyrate (C4) and the MCL fatty acids, sodium octanoate (C8), decanoic acid (C10), 10-undecenoic acid (C11:1, Δ10), and dodecanoic acid (C12). The repeating units of the PHAs produced were representative of the fatty acid substrate in number of carbons (Table 3). GC initially showed each of the PHAs to be comprised entirely of repeating units equal in number of carbons as the starting fatty acid. For example, samples grown in the presence of sodium octanoate were comprised of 3-hydroxyoctanoate repeating units and no other MCL repeating units.

The only potential contaminants seen in the initial GC experiments of samples produced from the medium chain fatty acids were seen as peaks with retention times that matched 3-hydroxybutyrate (3HB). To confirm the absence or presence of 3HB in the PHAs not produced from sodium butyrate, polymer samples were analyzed by HSQC-DEPT NMR (FIGS. 10-13).

FIG. 10 shows 1H-13C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxyoctanoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. Purified polymer was dissolved in deuterated chloroform and checked for contaminating repeating units, specifically poly(3-hydroxybutyrate) (PHB). CH and CH3 carbons are shown in black, and CH2 signals are shown in gray. In addition to comparison of the spectra with previous works of NMR on PHAs, the HSQC-DEPT NMR experiments were performed to confirm the absence of 3-hydroxybutyrate (3HB) repeating units in the various MCL-PHAs. 3HB within the polymer would be differentiated from the desired MCL repeating units by looking for the chiral carbon (the third carbon) and the methyl carbon's (the fourth carbon) signals. The chiral carbon of 3HB would be slightly shielded compared to that of the MCL-PHAs (67-68 ppm vs. 70-71 ppm) in the 13C portion of the spectra. The methyl carbon would give a black signal in the area of 1.30 ppm for 1H and 19-20 ppm for 13C. The signals in the poly(3-hydroxyoctanoate) sample that do not correspond to signals found in other MCL-PHA samples with fully saturated side chains are believed to be residual fatty acid substrate (octanoate) that was not fully removed from extracted polymer. This sample does not show detectable PHB.

FIG. 11 shows 1H-13C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxydecanoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. Purified polymer was dissolved in deuterated chloroform and checked for contaminating repeating units, specifically poly(3-hydroxybutyrate). CH and CH3 carbons are shown in black, and CH2 signals are shown in gray. In addition to comparison of the spectra with previous works of NMR on PHAs, the HSQC-DEPT NMR experiments were performed to confirm the absence of 3-hydroxybutyrate (3HB) repeating units in the various MCL-PHAs. 3HB within the polymer would be differentiated from the desired MCL repeating units by looking for the chiral carbon (the third carbon) and the methyl carbon's (the fourth carbon) signals. The chiral carbon of 3HB would be slightly shielded compared to that of the MCL-PHAs (67-68 ppm vs. 70-71 ppm) in the 13C portion of the spectra. The methyl carbon would give a black signal in the area of 1.30 ppm for 1H and 19-20 ppm for 13C. This sample does not show detectable PHB.

FIG. 12 shows 1H-13C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxy-10-undecenoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. Purified polymer was dissolved in deuterated chloroform and checked for contaminating repeating units, specifically poly(3-hydroxybutyrate). CH and CH3 carbons are shown in black, and CH2 signals are shown in gray. In addition to comparison of the spectra with previous works of NMR on PHAs, the HSQC-DEPT NMR experiments were performed to confirm the absence of 3-hydroxybutyrate (3HB) repeating units in the various MCL-PHAs. 3HB within the polymer would be differentiated from the desired MCL repeating units by looking for the chiral carbon (the third carbon) and the methyl carbon's (the fourth carbon) signals. The chiral carbon of 3HB would be slightly shielded compared to that of the MCL-PHAs (67-68 ppm vs. 70-71 ppm) in the 13C portion of the spectra. The methyl carbon would give a black signal in the area of 1.30 ppm for 1H and 19-20 ppm for 13C. The fatty acid substrates were found to have detectable amounts of different fatty acids in GC-MS experiments. This may have been the cause for detectable amounts of repeating units (GC-MS) in PHAs not equal in size to the starting substrate. The fatty acid substrate for production of poly(3-hyroxy-10-undecenoate) (PHUn), 10-undecenoic acid, was listed as 95% pure by the manufacturer (SAFC). The contaminating materials likely contain fully saturated fatty acids. These fully saturated fatty acids would also be incorporated into PHAs synthesized by this system. These potential contaminants can be seen in the 1H spectrum of the PHUn sample at −0.8 ppm. This is the same chemical shift seen for the terminal methyl groups of the polymers containing fully saturated side chains in the repeating units (FIGS. 10, 11 and 13). This sample does not show detectable PHB.

FIG. 13 shows 1H-13C HSQC-DEPT NMR spectroscopy results for poly(3-hydroxydodecanoate) synthesized in E. coli LSBJ expressing the pBBR-C1J4SII plasmid. Purified polymer was dissolved in deuterated chloroform and checked for contaminating repeating units, specifically poly(3-hydroxybutyrate). CH and CH3 carbons are shown in black, and CH2 signals are shown in gray. In addition to comparison of the spectra with previous works of NMR on PHAs, the HSQC-DEPT NMR experiments were performed to confirm the absence of 3-hydroxybutyrate (3HB) repeating units in the various MCL-PHAs. 3HB within the polymer would be differentiated from the desired MCL repeating units by looking for the chiral carbon (the third carbon) and the methyl carbon's (the fourth carbon) signals. The chiral carbon of 3HB would be slightly shielded compared to that of the MCL-PHAs (67-68 ppm vs. 70-71 ppm) in the 13C portion of the spectra. The methyl carbon would give a black signal in the area of 1.30 ppm for 1H and 19-20 ppm for 13C. This sample does not show detectable PHB.

Chemical shifts were compared to previous works utilizing NMR to analyze PHAs (28,51,52). Chemical shifts corresponding to the 3HB repeating unit were not observed in the NMR experiments (FIGS. 10-13).

Extracted PHAs were also analyzed by gas GC-MS, and MCL repeating units not equal in carbon length to the starting fatty acid were found. However, these minor fractions were in amounts lower than the GC detection limit and only qualitatively detected with GC-MS (Table 3). The carbon sources for the PHAs (i.e. fatty acids) were also analyzed by GC-MS, and the substrates were also found to have detectable amounts of contaminating fatty acids. Fully saturated fatty acids were present in large enough quantities in the 10-undecenoic acid substrate that the subsequent incorporation of the contaminants in the PHAs could be detected in HSQC-DEPT NMR of the poly(3-hydroxy-10-undecenoate) (PHUn) sample (see FIG. 12 and discussion of FIG. 12 above).

PHB was also synthesized using this system. Sodium butyrate was added to the cell media to generate the SCL PHA. Table 3 shows that PHB was accumulated at 7.81% of the cell dry weight in E. coli LSBJ. The PHB synthesized was not in large enough quantities for further purification and testing. After successful synthesis of PHB in this system, it is inferred that all repeating units can be synthesized and controlled from four and up to twelve carbons in length.

Physical and Thermal Properties of PHAs Synthesized from One Fatty Acid Substrate in E. coli LSBJ

To examine the physical properties of the PHA polymers produced, the weight average molecular weights (M_(w)), number average molecular weights (M_(n)), and polydispersities (M_(w)/M_(n)) were determined by GPC (Table 4). The decomposition temperatures (T_(d)) for each of the MCL PHAs produced were determined by TGA; and the glass-transition temperatures (T_(g)), melting temperatures (T_(m)), and crystallization temperatures (T_(c)) were determined by DSC. These results are shown in Table 4. The DSC and TGA scans for these samples are shown in FIGS. 14A-B.

FIGS. 14A-B show thermal analysis of purified medium-chain-length poly(3-hydroxyalkanoates). Differential scanning calorimetry scans are shown in (A), and thermogravimetric analysis results are shown in (B) for purified poly(3-hydroxyoctanoate) (PHO), poly(3-hydroxydecanoate) (PHD), poly(3-hydroxy-10-undecenoate) (PHun), and poly(3-hydroxydodecanoate) (PHDD).

PHA Copolymer Production in E. coli LSBJ

Four different groups of copolymers were synthesized in E. coli LSBJ carrying the pBBR-C1J4SII plasmid: poly[(R)-3-hydroxydodecanoate-co-(R)-3-hydroxydecanoate] (PHDDHD), poly[(R)-3-hydroxydodecanoate-co-(R)-3-hydroxyoctanoate] (PHDDHO), poly[(R)-3-hydroxydecanoate-co-(R)-3-hydroxyoctanoate] (PHDHO), and poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] (PHOHB). Synthesis of the desired sizes for the repeating units were achieved by incorporating fatty acid substrates that were equal in carbon atom length (for example, sodium octanoate was incorporated into PHAs as (R)-3-hydroxyoctanoate). The total mass of fatty acids fed during PHA copolymer production was 2 g L⁻¹. The mole ratios of the fatty acid substrates were altered in each experiment in an effort to ascertain correlations between specific substrate mole ratios and the corresponding mole ratios in the repeating units of the polymers. After incubation of the cells was complete, the mole ratios of the polymer produced were determined either by GC or NMR. Samples that were grown in the presence of only MCL substrates (sodium octanoate, decanoate, and dodecanoate) were analyzed by GC. The results for using dodecanoate and decanoate as PHA substrates at varying mole ratios are shown in Table 5. Results for the two other MCL copolymer systems (PHDDHO and PHDHO) are provided in Tables 6 and 7.

TABLE 5 Poly[(R)-3-hydroxydodecanoate-co-(R)-3-hydroxydecanoate] synthesis in E. coli LSBJ with varying mole ratios of fatty acid substrates Substrate ratio CDW^(a,b) PHA^(b) Composition (mol %)^(b,c) (C10:C12) (g L⁻¹) (mass %) 3HB 3HHx 3HO 3HD 3HDD 70:30 1.02 ± 0.06 26.3 ± 0.8 0 0 0 45.5 ± 0.6 54.5 ± 0.6 60:40 1.21 ± 0.03 22.4 ± 1.1 0 0 0 39.2 ± 0.6 60.8 ± 0.6 50:50 0.99 ± 0.08 29.5 ± 1.1 0 0 0 28.0 ± 0.8 72.0 ± 0.8 40:60 1.22 ± 0.05 21.1 ± 0.7 0 0 0 23.2 ± 1.0 76.8 ± 1.0 30:70 0.89 ± 0.04 26.1 ± 1.4 0 0 0 14.8 ± 0.1 85.2 ± 0.1 ^(a)Cell dry weight. ^(b)Values determined by GC. All values are averages of triplicate experiments plus or minus the standard deviations about those averages. ^(c)3HB, 3-hydroxybutyrate; 3HHx, 3-hydroxyhexanoate; 3HO, 3-hydroxyoctanoate; 3HD, 3-hydroxydecanoate; 3HDD, 3-hydroxydodecanoate.

TABLE 6 Poly[(R)-3-hydroxydodecanoate-co-(R)-3-hydroxyoctanoate] synthesis in E. coli LSBJ with varying mol ratios of fatty acid substrates Substrate Ratio CDW^(a,b) PHA^(b) Composition^(b,c) (mol %) (C12:C8) (g L⁻¹) (mass %) 3HB 3HHx 3HO 3HD 3HDD 30:70 1.21 ± 0.08 24.9 ± 3.4 0 0 27.6 ± 3.4  0 72.4 ± 3.4 40:60 1.21 ± 0.08 33.4 ± 3.5 0 0 16.2 ± 0.8  0.2 ± 0.0 83.5 ± 0.8 50:50 1.34 ± 0.13 25.2 ± 5.2 0 0 9.3 ± 0.6 0.2 ± 0.2 90.6 ± 0.7 60:40 1.36 ± 0.02 33.5 ± 1.3 0 0 7.4 ± 0.3 0.4 ± 0.1 92.2 ± 0.3 70:30 1.30 ± 0.09 21.4 ± 7.3 0 0 4.0 ± 0.2 0.2 ± 0.2 95.8 ± 0.3 ^(a)Cell dry weight. ^(b)Values determined by GC. All values are averages of triplicate experiments plus or minus the standard deviations about those average. ^(c)3HB, 3-hydroxybutyrate; 3HHx, 3-hydroxyhexanoate; 3HO, 3-hydroxyoctanoate; 3HD, 3hydroxydecanoate; 3HDD, 3-hydroxydodecanoate.

TABLE 7 Poly[(R)-3-hydroxydecanoate-co-(R)-3-hydroxyoctanoate] synthesis in E. coli LSBJ with varying mol ratios of fatty acid substrates Substrate Ratio CDW^(a,b) PHA^(b) Composition^(b,c) (mol %) (C10:C8) (g L⁻¹) (mass %) 3HB 3HHx 3HO 3HD 3HDD 30:70 1.03 ± 1.10 32.6 ± 2.5 0 0 55.0 ± 1.0 45.0 ± 1.0 0 40:60 1.25 ± 0.00 36.3 ± 1.7 0 0 42.6 ± 0.7 57.3 ± 0.8 0.1 ± 0.1 50:50 1.17 ± 0.08 25.3 ± 5.1 0 0 32.0 ± 0.8 68.0 ± 0.8 0 60:40 1.32 ± 0.20 34.5 ± 0.4 0 0 22.6 ± 1.1 77.3 ± 1.1 0.2 ± 0.0 70:30 1.22 ± 0.19 24.6 ± 1.7 0 0 15.2 ± 0.5 84.8 ± 0.5 0 ^(a)Cell dry weight. ^(b)Values determined by GC. All values are averages of triplicate experiments plus or minus the standard deviations about those average ^(c)3HB, 3-hydroxybutyrate; 3HHx, 3-hydroxyhexanoate; 3HO, 3-hydroxyoctanoate; 3HD, 3-hydroxydecanoate; 3HDD, 3-hydroxydodecanoate.

Given the mole ratios of the repeating units in the polymer determined by GC in Tables 5-7, the data were plotted to establish trend lines based on the initial fatty acid substrate ratios (FIGS. 15-17).

FIG. 15 shows the mol ratio of repeating units in poly[(R)-3-hydroxydodecanoate-co-(R)-3-hydroxydecanoate] polymer samples in relation to the starting fatty acid substrate ratio. The fatty acid substrates were used by E. coli LSBJ containing the pBBR-C1J4SII plasmid to synthesize PHAs as described in herein. All values were determined by GC and represent averages of three identical experiments. Error bars represent standard deviations about those averages.

FIG. 16 shows the mol ratio of repeating units in poly[(R)-3-hydroxydodecanoate-co-(R)-3-hydroxyoctanoate] polymer samples in relation to the starting fatty acid substrate ratio. The fatty acid substrates were used by E. coli LSBJ containing the pBBR-C1J4SII plasmid to synthesize PHAs as described herein. All values were determined by GC and represent averages of three identical experiments. Error bars represent standard deviations about those averages.

FIG. 17 shows the mol ratio of repeating units in poly[(R)-3-hydroxydecanoate-co-(R)-3-hydroxyoctanoate] polymer samples in relation to the starting fatty acid substrate ratio. The fatty acid substrates were used by E. coli LSBJ containing the pBBR-C1J4SII plasmid to synthesize PHAs as described herein. All values were determined by GC and represent averages of three identical experiments. Error bars represent standard deviations about those averages.

Each of the regressions was analyzed, and for each regression analysis, the slopes of the lines were found to be statistically different than zero (p-value ≤0). These trend lines allow for the prediction and production of MCL PHA copolymers with desired and specific final mole ratios of repeating units. As an example, we attempted to synthesize a PHDDHD copolymer with a repeating unit mole ratio of 65:35 C12:C10. Based on the trend for PHDDHD copolymers (FIG. 15), it was predicted that a C12:C10 substrate ratio of 43.1:56.9 could be used to produce a PHDDHD copolymer with a mole ratio of 65:35 C12:C10 for the repeating units. Cells were grown with this ratio of C12:C10 substrate fatty acid substrates, and after incubation of the cells with the substrates and analysis by GC, the polymers produced were found to have 64.0±1.4 mol % (R)-3-hydroxydodecanoate and 36.0±1.4 mol % (R)-3-hydroxydecanoate repeating units by GC. This result clearly demonstrates our ability to produce MCL PHA copolymers with defined ratios of repeating units.

Due to the structural differences between the (R)-3-hydroxyoctanoate and (R)-3-hydroxybutyrate, the mole ratios for the SCL-co-MCL copolymer PHOHB were determined by ¹H NMR as opposed to GC. MCL PHA repeating units cannot be differentiated from one another via ¹H NMR, and therefore GC was used for quantification of the PHDDHD, PHDDHO, and PHDHO samples. GC was used to confirm that only (R)-3-hydroxyoctanoate and (R)-3-hydroxybutyrate were present in the PHOHB polymer. After initial GC experiments, PHAs were extracted from the cells and analyzed by ¹H NMR. A sample spectrum is shown in FIG. 8. All peak integrals were standardized against the signal at 0.90 ppm, representing the terminal methyl protons of the (R)-3-hydroxyoctanoate repeating unit. Setting this peak integral value to 3.0 allowed for determination of repeating unit mole ratios by subtracting the expected integral values for (R)-3-hydroxyoctanoate from the remaining peaks. This process was carried out for PHOHB copolymers produced from C8:C4 substrate ratios of 2:98, 5:95, 10:90, 15:85, and 20:80, and the repeating unit mole ratios in the polymers are provided in Table 8.

As was done for the PHDDHD system, the correlation between feedstock ratio and resulting repeating unit ratio in PHOHB was quantified based on the compositional data collected for the PHOHB copolymers (FIG. 18).

FIG. 18 shows the mol ratio of repeating units in poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] polymer samples in relation to the starting fatty acid substrate ratio. The fatty acid substrates were used by E. coli LSBJ containing the pBBR-C1J4SII plasmid to synthesize PHAs as described herein. All values were determined by NMR and represent averages of integrations at peak shifts at 1.3, 2.6, and 5.2 ppm in 1H NMR spectra. These integrations were compared to the integration at 0.9 ppm, which was set to 3.0 (see FIG. 2 for details). Error bars represent standard deviations about those averages.

The ¹H NMR experiments showed a linear trend of repeating unit mole ratios compared to starting substrate mole ratios. Analysis of the regression again showed that the slope was statistically different from zero (p-value ≤0). Based on this correlation, it was predicted that a PHOHB copolymer containing a ratio of 10:90 C8:C4 could be synthesized in E. coli LSBJ if the strain was grown with a fatty acid substrate mole ratio of 3.3:96.7 C8:C4. Carrying out an experiment with this substrate ratio lead to the production of a polymer with a C8:C4 ratio of 9.8:90.2 (Table 8). These results show unprecedented precision in the control over repeating unit ratios for both SCL-co-MCL PHAs and MCL PHA copolymers.

GPC of Select PHA Copolymers Produced in E. coli LSBJ

The molecular weights of each of the PHOHB copolymers were estimated by GPC. As shown in Table 8, both the weight average (M_(w)) and number average (M_(n)) molecular weights of the polymers decreased with an increasing concentration of 3HO repeating units. The polydispersities (defined as the ratio of M_(w) to M_(n)) for the PHOHB samples were typically between 2.44 and 2.80. The yield of PHA produced as a percent of the dry cell mass tended to be greater when the 3HO concentration exceeded 10%.

TABLE 8 Polymer yield and molecular weight estimates for various poly[(R)-3- hydroxyoctanoate-co-(R)-3-hydroxybutyrate] samples Substrate Polymer ratio CDW PHA (mass ratio (C8:C4) (C8:C4) (g L⁻¹) %) M_(w) (kDa) M_(n) (kDa) M_(w)/M_(n)  2:98  6.4:93.6 1.49 ± 0.03 36.4 ± 2.6 417 ± 4 171 ± 5 2.44 ± 0.05  3.3:96.7  9.8:90.2 1.70 ± 0.07 38.8 ± 3.1 390 ± 8 139 ± 5 2.80 ± 0.10  5:95 14.7:85.3 1.63 ± 0.05 47.0 ± 2.7 379 ± 9 154 ± 6 2.46 ± 0.03 10:90 21.8:78.2 1.52 ± 0.03 51.5 ± 5.5 333 ± 4 127 ± 5 2.62 ± 0.09 15:85 31.3:68.7 1.35 ± 0.38 48.9 ± 0.2 323 ± 4 121 ± 3 2.68 ± 0.03 20:80 38.0:62.0 1.61 ± 0.04 51.8 ± 4.6 301 ± 2 114 ± 1 2.65 ± 0.01 CDW, cell dry weight; M_(w), weight average molecular weight; M_(n), number average molecular weight; M_(w)/M_(n), polydispersity. Polymer C8:C4 ratios were determined by ¹H NMR. CDW and PHA yield determined by gas chromatography. Molecular weights determined by gel permeation chromatography. Values represent averages of triplicate experiments plus or minus standard deviations about those averages.

Thermal Properties of Select PHA Copolymers Produced in E. coli LSBJ

DSC and TGA experiments were carried out to determine the degradation, glass-transition, crystallization, and melting temperatures (Table 9 and FIGS. 19 and 20).

FIG. 19 shows TGA scans of various poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] samples synthesized in E. coli LSBJ containing the pBBR-C1J4SII plasmid.

FIG. 20 shows DSC scans of various poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] samples synthesized in E. coli LSBJ containing the pBBR-C1J4SII plasmid.

The degradation temperatures for all PHOHB samples were between 214° C. and 235° C. Analysis of the DSC scans for the PHOHB samples with between 6 and 15% (R)-3-hydroxyoctanoate (3HO) repeating units showed a single glass-transition temperature, which was always above the reported glass-transition temperatures for poly[(R)-3-hydroxyoctanoate] (PHO) (44,53) and below the more commonly reported value of 4° C. for poly[(R)-3-hydroxybutyrate] (PHB) (29). As the concentration of the 3HO repeating units was increased above 15% in the PHOHB copolymers, two distinct glass-transitions were observed. The glass-transition peak at the lower temperature decreased with increasing amounts of 3HO present in the polymer (Table 9), approaching but not reaching previously reported values for PHO (44,53). The second glass-transition temperature was between −4.5° C. and −6.3° C.

TABLE 9 Poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] repeating unit mole ratios and thermal properties Substrate ratio Polymer ratio T_(m) (C8:C4) (C8:C4) T_(d) (° C.) T_(g) (° C.) T_(c) (° C.) (° C.)  2:98  6.4:93.6 230 −1.42 65.1 158  3.3:96.7  9.8:90.2 214 −2.34 67.0 158  5:95 14.7:85.3 217 −2.23 77.6 156 10:90 21.8:78.2 234 −20.8/−4.89 77.3 157 15:85 31.3:68.7 229 −26.0/−6.24 n.d. n.d. 20:80 38.0:62.0 220 −29.0/−5.32 n.d. n.d. T_(d), degradation temperature; T_(g), glass-transition temperature; T_(c), crystallization temperature; T_(m), melting temperature; n.d., not detected.

Melting and crystallization events were observed in PHOHB samples that contained between 6 and 22% 3HO, but not in those for which the 3HO content was greater than or equal to 31.3% (Table 9) and FIG. 20). The observed melting temperatures that were observed were between 157° C. and 160° C. The crystallization temperatures increased with increasing amounts of 3HO in the copolymer. The lowest crystallization temperature was 65.1° C., corresponding to the lowest amount of 3HO (6.4%), and the values increased to approximately 77° C. before the exothermic events were no longer observed.

Tensile Strength Analyses of Select PHA Copolymers Produced in E. coli LSBJ

Tensile strength tests were performed on the PHOHB samples to investigate the correlation between physical properties and compositional changes in the repeating units. Initially, the samples that were generated with substrate C8:C4 ratios of 2:98, 5:95, 10:90, and 15:85 were analyzed as described above in Materials and Methods. The polymer generated from a C8:C4 20:80 substrate ratio was too tacky and amorphous to punch consistent dogbone samples without stretching the polymer significantly prior to analysis. However, for other C8:C4 ratios, Young's modulus, yield strength, ultimate tensile strength, and strain to failure (elongation to break) were determined from the stress-strain curves (FIGS. 9 and 21).

FIG. 21 shows examples of data generated in tensile strength analysis experiments with poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] samples. Most samples resembled the top chart, which is one example of data from the samples with a mol ratio of 14.7:85.3, (R)-3-hydroxyoctanoate to (R)-3-hydroxybutyrate. The samples that contained a mol ratio of 31.3:68.7 (R)-3-hydroxyoctanoate to (R)-3-hydroxybutyrate were smoothed with a 5-point rolling average (bottom chart) of the raw Stress values (middle chart).

The ability to accurately resolve data points in these experiments was compromised once the C8:C4 ratio reached 31.3:68.7 (the sample created with the 15:85 C8:C4 substrate ratio). Data collected from these polymer samples were smoothed with a five-point rolling average to estimate the parameters shown in FIGS. 9A-D (see also FIG. 21 and discussion of FIG. 21 above).

After these initial tensile strength analyses were completed, the PHOHB polymer containing a C8:C4 repeating unit ratio of 9.8:90.2 was also tested. The Young's modulus and strain to failure were in between those of the PHOHB polymers with C8:C4 repeating unit ratios of 6.4:93.6 and 14.7:85.3. The values for the yield strength and the ultimate tensile strength closely resembled those for the PHOHB polymer with a repeating unit ratio of 6.4:93.6. There was a notable drop off in the yield strength and ultimate tensile strength once the 3HO concentration moved to ≥14.7 mol % of the copolymer (or above 9.8 mol %).

The physical properties were further analyzed for statistical relevance using analysis of variance (ANOVA) tests with the software program MINITAB. There were strong correlations among the four physical properties that were measured, and ANOVA tests showed that the means were statistically different (p-value of <0.001). To gain a better understanding of the relationship between the variables as a function of the C8:C4 ratios, linear regression followed by further analysis of variance was used. The plots generated showed that the trends seen in FIGS. 9A-D are statistically significant. This confirms that the different C8:C4 ratios conferred statistically different strength and modulus values (Table 10 and FIGS. 22 and 23).

TABLE 10 Correlations of poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] physical property values. Elongation Young's modulus Yield strength to break Yield strength 0.923 Ultimate tensile 0.937 0.993 strength Elongation to break −0.795 −0.783 −0.779

To gain a better understanding of the relationship between the variables as the C8:C4 ratios changed, linear regression followed by further analysis of variance was used. Using the Young's modulus data as an example, the percent 3HO (C8) in the polymer was used as the “x” variable, and the natural logarithm of the physical property values were used as the “y” variable. Using the software program MINITAB, a plot for the linear regression described was generated for the Young's modulus experiments (FIG. 17). Analysis of variance was used again to test if the regression relationship is statistically significant. The p-value for this analysis was <0.001, indicating the relationship is statistically significant and the Young's modulus did decrease with increasing amounts of 3HO in the PHOHB polymers. This same process was repeated for ultimate tensile strength data as well (FIG. 18), and again the p-value for this analysis was <0.001, indicating the relationship is statistically significant.

FIG. 22 shows a plot of the natural logarithm of Young's modulus values (LN_Yng) against the percent of 3HO (C8_Proportion) in of poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] samples. Plot was made in the MINITAB software program.

FIG. 23 shows a plot of the natural logarithm of ultimate tensile strength values (LN_Tens) against the percent of 3HO (C8_Proportion) in of poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] samples. Plot was made in the MINITAB software program.

FIGS. 24A-C show 1H NMR spectra of poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] containing 68.7% PHB prior to and after acetone fractionation. A, sample spectra prior to acetone fractionation. B, acetone soluble fraction. C, acetone insoluble fraction.

FIG. 24A shows a PHOHB sample made from a 15:85 C8:C4 substrate ratio and containing 68.7% PHB. FIGS. 24B and 24C shows the spectra of the sample after acetone fractionation. FIG. 24B is the acetone soluble fraction and was found to have 54.9% PHB, and FIG. 24C is the acetone insoluble fraction and was found to have 80.1% PHB. PHB homopolymer is insoluble in acetone, and finding it in such a high concentration (54.9%) in the acetone soluble fraction indicates that the PHOHB produced here is not a blend of PHO and PHB homopolymers but an actual copolymer. DSC was also used to check for any changes in thermal properties after acetone fractionation. The same samples used to generate FIGS. 24A-C were analyzed as described herein, and the results are shown in FIG. 25. The glass-transition temperatures, crystallization temperatures, and melting temperatures for these scans are summarized in Table 11.

TABLE 11 Thermal properties of poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] containing 68.7% PHB before and after acetone fractionation Sample Tg (° C.) Tc (° C.) Tm (° C.) unfractionated −26.0/−6/24 n.d. n.d. acetone soluble acetone insoluble −27.1/−4.61 82.1 144 Tg, glass-transition temperature; Tc, crystallization temperature; Tm, melting temperature; n.d., not detected.

FIG. 25 shows DSC results of poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate] containing 68.7% PHB before and after acetone fractionation. Scans were offset for clarity. The top scan is of the sample prior to acetone fractionation. The middle scan is the acetone soluble fraction. The bottom scan is the acetone insoluble fraction.

Statistical Analysis of Physical Properties Data

The correlations among the four physical property values generated for the PHOHB (Young's modulus, strain to failure, yield strength, and ultimate tensile strength) were very high (Table 10). Based on these correlations, it was assumed that further analysis of one of these sets of values would be nearly identical to analysis of the others. Analyses of variance tests were used to determine if the mean values for the physical properties of the polymers with different C8:C4 values were the same. Using both parametric analysis of variance after taking the natural logarithm of the data and a Kruskal-Wallace test, the values were found not to be identical (i.e., p-values for all variables for both analyses were <0.001).

To gain a better understanding of the relationship between the variables as the C8:C4 ratios changed, linear regression followed by further analysis of variance was used. Using the Young's modulus data as an example, the percent 3HO (C8) in the polymer was used as the “x” variable, and the natural logarithm of the physical property values were used as the “y” variable. Using the software program MINITAB, a plot for the linear regression described was generated for the Young's modulus experiments (FIG. 17). Analysis of variance was used again to test if the regression relationship is statistically significant. The p-value for this analysis was <0.001, indicating the relationship is statistically significant and the Young's modulus did decrease with increasing amounts of 3HO in the PHOHB polymers. This same process was repeated for ultimate tensile strength data as well (FIG. 18), and again the p-value for this analysis was <0.001, indicating the relationship is statistically significant.

Acetone Fractionation

To determine if the PHA copolymers PHOHB synthesized in this study were copolymers and not blends of PHO and PHB, the samples were subjected to acetone fractionation. Films of PHOHB were placed in a 50 ml centrifuge tube with 20 ml of acetone and incubated at 50° C. with gentle rocking. Samples were allowed to cool to room temperature, and the liquid portion of the mixture was filtered with a 0.45 μm PTFE filter under vacuum. The remaining solid polymer was rinsed twice with a small volume (2-5 ml) of acetone both the filtrate (acetone soluble) and acetone insoluble fractions were allowed to dry. Samples were then subjected to 1H NMR analysis as described herein. An example of the spectra seen is displayed in FIG. 19.

Additional Examples of PHA Copolymer Production

FIG. 32 shows the results of poly[(R)-3-hydroxy-10-undecenoate-co-(R)-3-hydroxyoctanoate] production in E. coli LSBJ harboring the pBBR-C1J4SII plasmid. The (R)-3-hydroxy-10-undecenoate repeating units were provided by 10-undecenoate, and the (R)-3-hydroxyoctanoate repeating units were provided by sodium octanoate. The relative concentration of fatty acid substrates was varied to demonstrate the corresponding change in terminal alkenes present in the polymer product (determined by NMR). This plot portrays the change in concentration of terminal alkenes in repeating units of PHAs as a function of starting concentration of 10-undecenoate relative to total fatty acid content.

FIG. 33 shows a 300 MHz ¹H NMR spectrum of a poly[(R)-3-hydroxy-10-undecenoate-co-(R)-3-hydroxyoctanoate] sample synthesized by E. coli LSBJ carrying the pBBR-C1J4SII plasmid. Cells were grown with 10-undecenoate and sodium octanoate at a mole ratio of 50:50 to generate this sample. 3HUnΔ10, (R)-3-hydroxy-10-undecenoate; 3HO, (R)-3-hydroxyoctanoate. Based on the peak integrations, it was determined that this polymer had a 3HUnΔ10:C8 mole ratio of 72.3:27.7.

The ratio of PHA to PLA in the copolymers produced is controlled in a manner similar to what has been established for PHA copolymers (see Example 2). Thus PHA-co-PLA copolymers are produced by the methods disclosed herein above with defined compositions, allowing for control over the material properties of next generation of biobased, biodegradable plastic materials.

Discussion

The E. coli LSBJ strain developed here in combination with the PHA production plasmid pBBR-C1J4SII allows strict control of repeating unit composition of PHAs for both MCL and SCL PHAs. The desired PHAs can be synthesized based simply on selecting a fatty acid substrate of equal carbon length. As shown in Table 3, PHAs were synthesized in sizes ranging from four to twelve carbons. Because of the native enoyl-CoA hydratase knockouts, the resulting PHAs were near homopolymers based on GC, GC-MS, and NMR analysis. GC-MS analysis found detectable amounts of repeating units different in carbon lengths than the starting fatty acid substrate, but this is likely due to impurities of the substrates and media (Table 3).

E. coli LSBJ is particularly useful for MCL PHA production from fatty acids. The parent strain, E. coli LS5218, is known to have increased uptake and metabolism for a broad range of fatty acids without the need for transcriptional induction of the appropriate fad genes by FadR (17,18). The PhaJ4 and PhaC1(STQK) introduced to synthesize the PHAs have high activity towards MCL substrates. This allowed for the strain to generate MCL PHAs at 25% cell dry weight or greater (Table 3).

Although the system created here was designed with MCL PHA repeating unit control in mind, control can also be extended to SCL PHAs. The advantages of E. coli LSBJ extend to SCL sized fatty acids, and thus PHB was synthesized from sodium butyrate. This was expected as the uptake for shorter fatty acids does not need to be induced in this strain (18), and the PhaJ4 and PhaC1(STQK) enzymes selected for PHA synthesis have reported or inferred activity for four carbon substrates (21,22,26). The PHB yield was much lower than the MCL PHAs. This could be because of enzyme substrate specificity favoring MCL substrates.

This system offers the broadest range of repeating unit control of PHAs in a single organism. Other work has been reported to control repeating unit composition in Pseudomonas putida KT2442 (8). The polymer yields here were lower compared to the Pseudomonas system developed by Wang et al., but the Pseudomonas system was unable to synthesize homopolymers with repeating units greater than seven carbons. The E. coli LSBJ system developed in this study was shown to maintain repeating unit control up to and including repeating units of twelve carbons. The size limits for repeating unit control of this system have not been determined as of this writing.

The thermal properties of the MCL PHAs produced here have similarities and differences when compared to other works that have produced MCL PHA homopolymers (8,9). Poly(3-hydroxyoctanoate) (PHO), for example, showed a T_(g) of −42.1° C., which is lower than other works producing polymers of ≥98 mol % PHO (8,9). No melting temperature was seen here for PHO, which is not the case for reports of the other studies with ≥98 mol % PHO polymers (8,9). However, the other studies report very different melting temperatures. The study by Wang et al. reports a melting temperature of 66.06° C., but a melting endothermic peak cannot be seen on the DSC curve (8). The study by Rai et al. reported a range of melting temperatures between 39° C. and 50° C., but DSC curves are not presented (9). DSC curves for this work are shown in FIGS. 14A-B. There are many differences in these works for producing, purifying, and characterizing the polymers, which may account for the lack of consistency in reported thermal properties of PHO homopolymers.

Another MCL PHA homopolymer that has been synthesized before this work and was produced here was poly(3-hydroxydecanoate) (PHD) (7). Both the work presented here and the work by Liu et al. report glass transitions between −37° C. and −40° C. The previously produced PHD homopolymer had a reported melting temperature of 72.2° C. compared to the melting temperature of 57.7° C. for the PHD produced in this work. Once again, differences in synthesis, purification, and characterization methods could play a role in the differences in reported thermal properties.

More MCL PHA homopolymers need to be synthesized and characterized to determine if melting, crystallization, and glass transition temperatures can be predicted based on repeating unit size in PHAs. The T_(g) for these polymers may decrease with increasing side chain length as has been suggested by another work developing MCL PHA homopolymers (8). If this is true, the glass-transition temperature for poly(3-hydroxydodecanoate) (PHDD) may be near or below the −80° C. minimum of the DSC scans performed. There was no definitive trend in the degradation temperatures of all the polymers tested (Table 4 and FIGS. 14A-B). The thermal properties and trends of polymers here did not coincide with the properties of the polymers reported by Wang et al (8). However, this could be the result of differing polymer purification, polymer storage conditions, molecular weights, and/or experimental conditions for DSC and TGA.

The molecular weights varied for the MCL PHAs, and the polydispersities determined by GPC ranged from 1.6 to 3.1 (Table 4). The molecular weights (M_(n)) were varied with PHD and PHO having noticeably larger molecular weights than PHUn and PHDD. The PHO molecular weight (M_(n)) and polydispersity are comparable to previous work with this polymer (9). The PHD molecular weight (M_(n)) is more than twice as small as a previous work producing PHD homopolymer, and the polydispersity is greater in this example (7). The PHUn and PHDD homopolymers have not been synthesized bacterially prior to this work.

Comparison of polymer molecular weights and polydispersities from this work to other works is difficult due to the differences in bacterial production. Other works have relied on native Pseudomonas PHA synthesis enzymes. The polymers here were made in E. coli expressing an enzyme with naturally broad substrate specificity (PhaJ4) and an enzyme with engineered broad substrate specificity [PhaC1(STQK)]. The substrate specificities of the PHA synthesis enzymes, particularly the PHA synthase, could have a significant effect on the molecular weights of the polymers. Some in vitro characterization of the substrate specificity of the PhaC1(STQK) synthase used in this example is available but limited to substrates with four to ten carbons (54). Because the relationship of substrate specificity to molecular weight of the polymer produced by PHA synthase enzymes is not completely defined, there is not sufficient material to draw conclusions as to why there are differences in the molecular weights of the PHA polymers produced in this example. However, the differences in the substrate specificity of engineered and native PHA synthases may be responsible for the differences in the molecular weights of polymers.

The strain E. coli LSBJ was developed to allow for repeating unit control in PHA biosynthesis. Knockouts of the genes fadB and fadJ created a dam in the β-oxidation pathway (FIG. 1). Instead of being degraded, fatty acid substrates were converted to PHAs by PhaJ4 and PhaC1 (STQK). This allowed for control of repeating unit composition simply by selecting the fatty acid that matches the desired repeating unit in carbon length. This strain successfully demonstrated repeating unit control in PHAs with repeating units of four, eight, ten, eleven, and twelve carbons. This represents the first bacterial strain to achieve such diversity in repeating unit control of PHAs.

After characterization of polymers synthesized from a single fatty acid substrate was complete, E. coli LSBJ was used to synthesize PHA copolymers from two fatty acid substrates. Shown here is a demonstration of the first bacterial system to produce PHA copolymers with specifically defined and tunable repeating unit ratios. Precise control was shown for both MCL and SCL-co-MCL PHA copolymers by calibrating various feedstock ratios against specific copolymer repeating unit ratios as described herein, and by successfully and accurately producing copolymers with pre-selected repeating unit ratios within the calibrated range. This was completed with both the MCL PHDDHD copolymer by synthesizing a 65:35 C12:C10 copolymer and the SCL-co-MCL PHOHB copolymer by synthesizing a 10:90 C8:C4 copolymer.

It has been reported that SCL-co-MCL PHAs have physical properties that are similar to petroleum-based polymers such as polypropylene and polyethylene (see Table 12) (1,10,55). The thermal and physical properties of the series of PHOHB copolymers synthesized in this study were examined to compare our SCL-co-MCL PHA with common petroleum-based plastics. The degradation temperatures determined by TGA experiments did not reveal a notable trend (Table 9 and FIG. 19). During DSC experiments, however, two glass-transitions became apparent as the concentration of the 3HO repeating units increased in the PHOHB copolymers. The glass-transition peak at the lower temperature decreased with increasing amounts of 3HO present in the polymer (Table 9), approaching but not reaching previously reported values of PHO (44,53). It may be that as the 3HO content continues to increase, the polymer comes to progressively resemble the PHO homopolymer more and more closely. The second glass-transition temperature was between −4.5° C. and −6.3° C. Although the trend is not definitive, this transition may be moving farther away from the glass-transition of the PHB homopolymer as the amount of 3HO in the PHOHB copolymer increases.

TABLE 12 Comparison of thermal and physical properties of PHA samples produced in this study to common polymers Melting Glass-transition Young's Tensile Strain to temperature temperature modulus strength failure Polymer (° C.) (° C.) (MPa) (MPa) (%) Reference PHB 171 4.50 185 5.3 6.0 This study* PHOHB 158 −1.42 170 10.5 53 This study (6.4% 3HO) PHOHB 159 −2.23 31.5 3.2 110 This study (14.7% 3HO) PHOHB n.d. −26.0/−6.24 12.0 1.7 230 This study (31.3% 3HO) Polypropylene 176 −10 1700 38 400 (2) Low-density 130 −30 200 10 620 (2) polyethylene PHB, poly[(R)-3-hydroxybutyrate]; PHOHB, poly[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxybutyrate]; n.d., not detected. *PHB was synthesized from sodium butyrate as described herein.

The two glass-transition temperatures may also be indicative of block-like sequences of PHB and PHO within the polymer or mixtures of homopolymer PHO and PHB. To investigate this, various PHOHB samples were subjected to acetone fractionation given that PHB is insoluble in acetone, and PHO is soluble. A portion of each sample dissolved in the acetone, and the soluble and insoluble fractions were analyzed by ¹H NMR spectroscopy. We found evidence of both PHO and PHB in each fraction for each PHOHB sample tested. Therefore, for there to be PHB in the acetone soluble fraction, we believe it must be covalently bound to PHO. Also, the PHO that did not dissolve in acetone must likewise be attached to a large fraction of PHB. The different fractions had very different repeating unit compositions (55% PHB to 45% PHO for acetone soluble copolymer, 80% PHB to 20% PHO for acetone insoluble copolymer) than prior to the fractionation, meaning there is a mixture of different copolymers that comprise the entire polymer population.

The melting and crystallization characteristics of the PHOHB copolymers were also sensitive to changes in the repeating unit ratios. The crystallization temperatures increased with increasing amounts of 3HO and moved away from reported values of approximately 51° C. for PHB (2,29).

When the amount of 3HO in the copolymer reached 31.3 mol %, a crystallization event was no longer observed in DSC experiments. The same absence was observed for samples with 3HO concentrations above 31.3 mol %. Below this mole percentage of 3HO, the melting temperatures of the copolymers were consistently slightly below 160° C. Although there was no obvious trend for the differences in melting temperatures, for those polymers where melting events were observed, all melting temperatures fell below what has been observed by us and others for PHB (see references and Table 5) (2,4,29,40) and were at least 50° C. below the degradation temperatures. The differences observed for melting temperatures and crystallization temperatures have important ramifications for polymer processing. It is highly desirable to have polymers with melting temperatures far below the degradation temperature and crystallization temperatures closer to melting temperatures to improve the residence time during polymer processing. Overall, the results summarized in Table 9 establish an ability to calibrate and select for specific thermal properties that may be desired in the context of a given application.

In addition to demonstrating control over thermal properties, this system allows for specific physical properties to be selected when synthesizing these biodegradable copolymers. The tensile strength analyses showed that statistically relevant trends existed for the Young's modulus, ultimate tensile strength, yield strength, and elongation to break as functions of the ratio of 3HO to 3HB in the PHOHB copolymers (FIGS. 9A-D). The PHOHB sample that was produced after the initial copolymers, which was designed to have a 10:90 C8:C4 ratio, had physical properties that followed the developed trends. Therefore, we conclude that PHA copolymers can be synthesized with desired repeating unit ratios and sets of physical properties by simply selecting the appropriate starting substrate ratio. These physical properties are compared to those of common, petroleum-based polymers in Table 5.

An unexpected trend was observed in the results of GPC experiments for determining the average molecular weights of the PHOHB copolymers (Table 8). Both the weight average (M_(w)) and number average (M_(n)) decreased with increasing percentages of 3HO repeating units. The enzymes expressed in E. coli LSBJ to synthesize PHAs, PhaJ4 and PhaC1(STQK) (FIG. 1), have broad substrate specificities but prefer MCL sized substrates (21,22,26). The PhaC1(STQK) enzyme has been reported to produce lower molecular weight PHA products than its parent enzyme from P. sp. 61-3 (56), and other mutations have been shown to increase molecular weights produced by this enzyme (57-59). However, based on the preferred substrate size of the enzyme, it was expected that increasing the percentage of 3HO in the copolymers would lead to an increase in the average molecular weight. What was observed instead was a decrease in molecular weights as the 3HO repeating unit fraction of the copolymer increased. Previous work has shown that PHB produced with the PhaC1(STQK) enzyme in E. coli was around 490 kDa (M_(n)) (54). The average molecular weights appear to be moving toward what has been reported for homopolymer PHB from this enzyme as the amount of 3HB in the copolymer increases (Table 8). Similarly, the molecular weights move toward molecular weights observed when a near homopolymer of PHO was produced in E. coli LSBJ (44). Molecular weight of polymers is another determining factor in the physical properties of PHAs, and there are recent and current efforts to consistently increase these values (60). These results indicate that molecular weights of PHA copolymers can also be controlled by the mole ratio of SCL to MCL repeating units incorporated into the polymer.

Example 2 References

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6.3 Example 3: Engineering E. coli for Improved Production of Polyhydroxyalkanoate (PHA)-based Biodegradable Plastics

Introduction

This example sets forth systems and methods for increasing the production of SCL-MCL and other novel PHAs. This example describes the improvement of PHA biopolymer production using metabolic engineering techniques to diversify the supply of monomers for PHA production and increase overall production of the polymers.

Materials and Methods

Gene Rearrangement to Control the Molecular Weight of PHA-based Polymers

Control over the molecular weights of polymers can have dramatic effects on the physical properties of the materials. Previous studies have demonstrated that gene order and thus enzyme expression levels are partially responsible for controlling the molecular weights of PHA polymers produced in E. coli. PHB polymers have been produced with ultra-high-molecular-weights using gene rearrangement (38). It is also known that PHA synthase activity is a key factor for controlling the molecular weight of PHA (104). This method for controlling the molecular weights of PHA-co-polylactic acid (PLA) copolymers is shown in FIG. 29. In addition to producing polymers with higher molecular weights, it is also important to produce PHA polymers with lower molecular weights. Many of the polylactic acid (PLA) biomaterials used in tissue engineering applications are of much lower molecular weights (typically 10³-10⁴ Da) (41, 101) than those the PHA polymers typically produced (typically 10⁵-10⁶ Da). High levels of expression of the phaC gene encoding the PHA synthase relative to monomer supplying enzymes result in the production of polymers with lower molecular weights.

To establish a range of molecular weights of PHA polymers produced in the engineered E. coli strains, expression constructs are assembled with the PHA synthase and various monomer supplying genes relative to a series of promoters with variable strengths (lac, ara, trc, R. eutropha native pha promoter) in large expression constructs using Gibson assembly (30, 31), a method well known in the art. Briefly, Gibson assembly allows for the one-step isothermal in vitro assembly of multiple DNA fragments regardless of fragment length or end compatibility. Placement of the phaC gene encoding the PHA synthase at the position nearest to the promoter results in PHA polymers with lower molecular weights while PHA polymers produced in constructs where the phaC gene was furthest from the promoter had the highest molecular weight (38).

Thus initial Gibson assembled constructs are made with the phaC1(STQK) gene occupying either the first or last position of the construct with genes encoding monomer-supplying enzymes shuffled into all other positions (FIG. 29). As an alternative, these constructs can be assembled via the OGAB method employed for SCL PHA production (38). These constructs are further engineered for varying levels of protein production by altering the ribosomal binding site using the RBS calculator designed and maintained by Howard Salis (salis.psu.edu/software/) (96, 97). The RBS calculator can be used to calculate the relative expression of each of the proteins based on designed RBS sites to alter translation from 0.5-fold to 100,000-fold higher than the original rate of translation within a factor of 2.3 (97).

The RBS calculator is then used to design primers with engineered RBSs for the pct, phaG, alkK, and phaC1 (STQK) by three increments (0.5, 10, and 100-fold of the native RBS). This results in controlled expression of the genes in the pathway, which leads to differences in the molecular weights of any polymers produced based on our previous study with the SCL PHA polymer PHB (38).

Production of PHA Polymers in Large Quantities with Defined Repeating Unit Compositions from Fatty Acids in E. coli

Engineering E. coli to Produce PHA Polymers from Fatty Acids

Pathways for the production of PHA polymers from fatty acids are shown in FIG. 26. It has been shown in Examples 1 and 2 that E. coli can be engineered to produce PHA from fatty acids. There are two main routes to convert β-oxidation intermediates to substrates for PHA production: (i) conversion of enoyl-CoA to (R)-3-hydroxyacyl-CoA substrates via (R)-specific-enoyl-CoA hydratases such as PhaJ (27, 99, 119-122), MaoC, PaaG, PaaF, and YdbU (86). The other route is via conversion of 3-ketoacyl-CoA to the (R)-3-hydroxyacyl-CoA substrates for polymerization using 3-ketoacyl reductases such as FabG (82, 94, 111) or RhlG (13). The major shortcoming of these studies is that β-oxidation of fatty acids leads to an uncontrolled repeating unit composition within the PHA polymers produced. Thus, there is little control over the physical properties of the PHA polymers produced. Also, to date, there has been only one study looking at the production of PLA-co-MCL PHA polymers (68), but it is expected that this class of polymers will possess desirable material properties in a similar way that SCL-MCL PHA copolymers do. These shortcomings have led to the impetus for the studies described in this example to produce engineered E. coli strains capable of producing a series of PHA and PHA-co-PLA copolymers with specifically defined repeating unit compositions in order to produce biopolymers with relevant material properties, such as improved ductility and toughness and clarity.

PHA-co-PLA copolymers are produced with defined repeating unit compositions by co-expressing the propionyl-CoA transferase (PCT) enzyme in the engineered E. coli LSBJ strain (115, 117). This strain is further engineered to improve yields of PHA by deletion of genes encoding negative transcriptional regulators (arcA and ompR) of the β-oxidation pathway in E. coli to increase substrate availability for PHA production. This leads to higher levels of production of both PHA polymers and copolymers and the production of PHA-co-PLA copolymers with defined repeating unit compositions.

Experimental Design

Deregulating Repression of β-oxidation in E. coli

E. coli LSBJ is used as the parental strain for the production of PHA polymers with defined repeating unit composition from fatty acids (FIG. 27). This strain utilizes the β-oxidation pathway to generate intermediates for PHA synthesis. To increase flux through this pathway, it is necessary to engineer or delete negative transcriptional regulators of β-oxidation and fatty acid uptake in E. coli. These have been identified in a previous study (25), and an outline of the modifications made in this example is shown in FIG. 30. Modifications include the deletion of genes encoding transcriptional regulators (fadR, arcA, and ompR) that downregulate expression of the fad regulon. E. coli LSBJ strain has been previously engineered that carries an inactivated fadR gene, constitutively expressed atoC, and inactivated fadB and fadJ genes for the production of PHA polymers with defined repeating unit compositions (115, 117, Examples 1 and 2). For this example, this strain is further engineered for optimized utilization of fatty acids as feedstocks for PHA polymer production by additionally inactivating the arcA and ompR genes using the λ-red system (117).

For example, LSBJ-arcA, LSBJ-ompR, and LSBJ-arcA-ompR E. coli strains are developed. PHA polymers are then produced in each strain as done for E. coli LSBJ, and polymer and growth yields calculated as previously described (115, 117).

ArcA has a regulatory role for the fad genes of E. coli (15). E. coli arcA mutants (71, 72) can be used for MCL PHA production.

OmpR has been shown previously to repress expression of fadL, which encodes the protein responsible for uptake of long-chain fatty acids in E. coli (37). To remove this repression, ompR is inactivated both independently and successively with arcA in E. coli LSBJ via λ-red recombination (18). Inactivation of these repressors leads to higher levels of both fatty acid substrate uptake and PHA production via the engineered β-oxidation pathway in E. coli LSBJ. Greater flux and uptake of the feedstocks of this pathway increase overall yields and decrease incubation times to produce PHA polymers from fatty acids.

Production of MCL PHA-co-PLA Polymers with Defined Repeating Unit Compositions

PHA homopolymers (117) and PHA copolymers (115) have been previously produced (see Examples 1 and 2) with defined repeating unit composition by supplying specific ratios of fatty acid feedstocks to the E. coli LSBJ strain (FIGS. 27 and 28). This control is extended to PHA-co-PLA copolymers by the co-expression of the pct gene with phaJ4 and phaC1(STQK) in E. coli LSBJ and co-feeds of specific concentrations of lactic acid and fatty acids (FIG. 31). The pct gene will be PCR amplified from the pTV118N plasmid (112), subcloned into the pBBR-C1J4SII vector (117) harboring the phaJ4 and phaC1(STQK) genes, and transformed into E. coli LSBJ. The transformed strain is grown in the presence of octanoate and lactic acid at various feed ratios (100:0, 90:10, 60:40, 50:50, 40:60, 10:90, and 0:100, octanoate:lactate) to produce MCL PHA-co-PLA copolymers with defined repeating unit compositions.

Example 3 References

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6.4 Example 4: In Vivo Incorporation of “Click”-ready Fatty Acids into poly-[(R)-3-hydroxyalkanoates] in Escherichia coli

Poly-[(R)-3-hydroxyalkanoate] biopolymers, or PHAs, are biocompatible and biodegradable polyesters produced natively by diverse microbial strains. PHA polymers have found widespread uses in applications ranging from sustainable replacements of non-biodegradable bulk-commodity plastics to biomaterials. However, further expansion into other markets and industries has generally been limited by the inability to chemically modify these polymers. This example demonstrates the engineering of E. coli LSBJ, a microbial strain able to produce PHA copolymers with controlled unit compositions from simple and accessible fatty acid feedstocks. The application spectrum of these materials can be broadened via production of chemically tractable PHA biopolymers containing “click”-ready chemical functionalities. This example also demonstrates the synthesis and biopolymerization of a panel of ω-azidofatty acids, and demonstrates their conjugation via a strain-promoted azide-alkyne cycloaddition (SPAAC) reaction. The convenience of accessing these materials creates new applications for functionalized PHA polymers.

Introduction

Poly-[(R)-3-hydroxyalkanoate] biopolymers, or PHAs, are biocompatible and biodegradable polyesters that can be produced by diverse microbial strains as carbon and energy reserves (1, 2). Generally, PHAs are classified according to the chain length of their repeating units. PHAs with chain lengths between three and five carbons long are known as short chain length (SCL) PHAs. Alternatively, if the polymer contains chain lengths between six and fourteen carbons long, the PHA is classified as medium chain length (MCL) (3, 4). Access to PHA copolymers consisting of a mixture of SCL and MCL chain length repeating units allow for the customization of their physical properties. This flexibility has permitted their successful application in areas ranging from sustainable replacements of non-biodegradable bulk-commodity plastics, to biomaterials and tissue engineering (5-7). However, a concerted expansion into other applications (e.g., advanced biomedical and targeted drug delivery applications) has been hindered by the intractable chemical nature of these polymers. Although biosynthetic production of PHAs by Pseudomonas oleovorans has yielded polymers with esters, terminal halides, aryl, and alkenyl groups, overall, this strain has limited control over the repeating unit sizes and thus limited control over the physical properties of the material (8-15). Functional group interconversions of PHA side chains, predominantly of polymers containing terminal alkenyl moieties, comprise the extent of current strategies available to fine-tune their physicochemical profiles and applications (16-22). Additionally, other approaches have centered on the chemical syntheses of polyhydroxybutyrate (PHB) (23), or biosynthetic circumvention via the elaborate multistep syntheses of chemically tractable polylactic or polyglycolic acids and their copolymers (24, 25). However, these methods are limited by the specificity of the catalysts used, resulting in the production of specific polymers with a limited range of physical properties.

This example discloses use of “click” chemical transformations. These “click” reactions comprise a group of synthetic, chemoselective strategies that have no side products, occur under mild reaction conditions, and proceed rapidly with high yields (26, 27). Methodologies such as UV-promoted thiol-ene click reactions between thiols and alkenes/alkynes haves been successfully employed in the conjugation of unsaturated PHAs (22, 28). Copper-catalyzed alkyne-azide cycloaddition reaction (CuAAC) is a click reaction that reliably yields 1,2,3-triazoles under mild conditions (29). The chemoselectivity of the CuAAC and its use in pharmaceutical applications has led to an expanded availability of commercial sources of azido- or alkynyl-conjugated biomolecules (30). To avoid the use of deleterious copper while preserving the kinetic parameters of the reaction in vivo, strain-promoted alkyne-azide cycloaddition (SPAAC) variants, with substituted cyclooctynes as their centerpiece, have been developed (31-33).

No examples are available of biosynthetically produced azido-PHAs from the in vivo uptake of synthetic azidofatty acids by engineered Escherichia coli. However, in vivo incorporation studies of azide-containing amino acids, sugars and lipids for protein labeling have been realized in both bacteria and eukaryotes alike (34-36).

This example discloses a panel of ω-azidofatty acids that can be incorporated into azido-PHA polymers in specific ratios by E. coli LSBJ strain (4, 6 and 10 in FIG. 34, Scheme 1, Syntheses of C8, C10 and C12 azido fatty acids) (37, 38). This microbial strain was engineered to produce PHA copolymers with controlled repeating unit compositions from simple and accessible fatty acid feedstocks (39). This example demonstrates the synthesis and in vivo polymerization by E. coli LSBJ of a homologous series of ω-azidofatty acids, and demonstrates the conjugation of novel biopolymers via a strain-promoted azide-alkyne cycloaddition reaction (SPAAC).

The synthetic routes to C8 and C12 ω-azidofatty acids were designed to exploit the cost-effective and synthetically adaptable nature of α,ω-diols (1 and 7 in FIG. 34, Scheme 1). Monobromination of commercially available diols 1 and 7 by treatment with 48% HBr in refluxing toluene afforded α,ω-bromohydrins 2 and 8 respectively. Subsequent nucleophilic displacement of 2 and 8 by reaction with NaN₃ in DMF (N,N-dimethylformamide) yielded the corresponding α,ω-azidoalcohols (3 and 9), which were cleanly oxidized to the target ω-azidofatty acids 4 and 10 by a methodology employing excess NMO (N-methylmorpholine-N-oxide) and catalytic amounts of TPAP in MeCN (acetonitrile) (40). Further, and with the purpose of expediting access to the target C10 ω-azidofatty acid (6), an abbreviated route was used involving the nucleophilic displacement of commercially available 10-bromodecanoic acid (5) with NaN₃ in DMF (N,N-dimethylformamide). All monomers were purified by SiO₂ flash column chromatography, and their purity confirmed by ¹H and ¹³C NMR before employing as a feedstock in all biopolymerization experiments.

Using established protocols, E. coli LSBJ was used to biosynthetically produce azido-PHA homopolymers comprising poly-[(R)-3-hydroxy-8-azidooctanoate] (PHON₃), poly-[(R)-3-hydroxy-8-azidodecanoate] (PHDN₃), and poly-[(R)-3-hydroxy-8-azidododecanoate] (PHDDN₃) homopolymers. All homopolymers were successfully produced and at sufficient yields to be characterized by ¹H, GPC, and FT-IR (for detailed experimental information see the Supporting Information below).

TABLE 1 PHODN₃ copolymers biosynthesized in E. coli LSBJ with varying mole ratios of fatty acid substrates Polymer mol Substrate mol ratio CDW^(a) PHA ratio^(b) (C8:C10N₃) (g L⁻¹) (mass %) (C8:C10N₃) 1:0.020 1.29 ± 0.01 20.0 ± 4.1 1:0.048 ± 0.003 1:0.053 1.12 ± 0.02 21.1 ± 1.2 1:0.118 ± 0.003 1:0.111 1.04 ± 0.01 17.4 ± 2.5 1:0.235 ± 0.005 1:0.250 1.21 ± 0.02 19.9 ± 4.1 1:0.470 ± 0.013 ^(a)Cell dry weight. ^(b)Values determined by ¹H NMR. All values are averages of triplicate experiments plus or minus the standard deviations about those averages.

Prompted by these results, and the realization that the utility of azido-PHAs will be enhanced by the ability to fine tune their composition, azido-MCL copolymers of poly-[(R)-3-hydroxyoctanoate] (PHO) were biosynthesized at different ω-azidofatty acid concentrations. Poly-[(R)-3-hydroxy-octanoate-co-(R)-3-hydroxy-8-azidooctanoate] (PHOON₃), poly-[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxy-10-azidodecanoate](PHODN₃), and poly-[(R)-3-hydroxyoctanoate-co-(R)-3-hydroxy-12-azidododecanoate] (PHODDN₃) were isolated in triplicate, purified and thoroughly characterized. These newly azido-PHA copolymers allow for tunable modification of the material dependent on the mol ratio composition of azido-PHA repeating units.

Owing to the susceptibility of organoazides to methanolytic conditions commonly employed prior to GC analyses (15% H₂SO₄ in MeOH), copolymer compositions and structural characterizations were performed via ¹H NMR and FT-IR spectroscopy. All copolymers isolated consistently displayed the characteristic triplet corresponding to the azide-bound methylene at roughly 3.25 ppm and a strong azide FT-IR stretch at 2096 cm⁻¹ (FIGS. 35A-B) correlating well with azide content. To normalize our spectra's integration the broad triplet located approximately at 0.88 ppm, corresponding to the methyl group in the (R)-3-hydroxyoctanoate (C8) repeating units, was employed. For the precise prediction of polymer compositions, standard curves were developed from ¹H NMR data obtained from all copolymer sets produced (see Supporting Information below).

Table 1 serves as an example of the results obtained with PHODN₃ copolymers produced from C8:C10N₃ substrate ratios of 1:0.020 (2 mol % C10N₃), 1:0.053 (5 mol % C10N₃), 1:0.111 (10 mol % C10N₃), and 1:0.250 (20 mol % C10N₃; 10 mM total fatty acid concentration). As opposed to PHOON₃ and PHODN₃, for the composition range studied, the standard curve for PHODDN₃ shows a polynomial trend. The data points collected however, and the trend in the polymer's C12 mol ratio, remained consistent with the values recently reported for PHODD. The combined substrate specificities of the biosynthetic pathway were clearly evidenced in the incorporation trend into their respective copolymers of C12N₃>>C10N₃>C8N₃ repeating units, in particular at greater fatty acid substrate concentrations (1:0.250 C8:CXN₃).

Molecular weights of all copolymers were determined by GPC (Supporting Information below). With the exception of PHODN₃ copolymers, which display a steady number average molecular weight (M_(n)) across all C10N₃ compositions, both PHOON₃ and PHDDN₃ generally exhibit a decreasing trend in M_(n) with increasing content in C8N₃ and C10N₃, respectively.

The thermal properties of the azido-PHAs isolated were found to be very similar to one another (Supporting Information below). Table 2 summarizes these results for the PHODN₃ copolymers produced in this study.

TABLE 2 Molecular weight and thermal properties of PHODN₃ copolymers Polymer mol ratio M_(n) ^(a) (kDa) T_(d1) T_(d2) T_(g) T_(c) T_(m) (C8:C10N₃) (PDI) (° C.) (° C.) (° C.) (° C.) (° C.) 1:0.048 ± 0.003 66 ± 1 (2.2 ± 0.1) 264.7 ± 5.8  460.3 ± 22.5 −42.1 ± 0.6 n.d. n.d. 1:0.118 ± 0.003 63 ± 2 (2.3 ± 0.1) 252.5 ± 7.1 457.9 ± 4.9 −43.8 ± 0.2 n.d. n.d. 1:0.235 ± 0.005 60 ± 2 (2.2 ± 0.1) 240.1 ± 7.1 464.5 ± 9.4 −45.4 ± 1.3 n.d. n.d. 1:0.470 ± 0.013 56 ± 3 (2.2 ± 0.1) 244.3 ± 2.3 465.2 ± 9.7 −47.3 ± 0.7 n.d. n.d. ^(a)Molecular weights obtained by GPC using CHCl₃ at 40° C. M_(n), number-average molecular weight; PDI, polydispersity index, T_(d), decomposition temperature; T_(g), glass-transition temperature; T_(c), crystallization temperature; T_(m), melting temperature; n.d., not detected.

TGA analyses were carried out a heating rate of 10° C./min, and revealed a trend consistent among all copolymers showing two distinct degradation temperatures (T_(d1) and T_(d2)) at approximately 250° C. and 460° C., respectively. T_(d1) is characteristic among PHAs and likely corresponds to volatilization of species from random chain scission via cis-elimination of the polyester backbone, while T_(d2) is clearly dependent on the azide content of the polymer and is possibly the product of evolution of nitrogenous species and N₂. DSC analyses obtained at a heating rate of 10° C./min after a slow cooling cycle at 5° C./min, showed a single glass-transition temperature (T_(g)) for each of the isolated copolymers with values ranging from −41.8° C. to −56.7° C., with the lower values correlating with an increase in the azide mol ratio of the copolymer. Neither T_(m) nor T_(c) were observed which in conjunction with the low T_(g) values, are suggestive of highly amorphous polymer materials.

To determine the ability of the polymer to conjugate via click methodologies, a small scale SPAAC reaction was performed of PHODN₃ (1:0.33 C8:C10N₃) with 1.0 equiv of commercially available BCN—OH 41R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-ylmethanol) (11, FIGS. 36A-B) (33). After mixing overnight, the reaction was monitored by assessing the complete shift of the C10N₃-10 signal from 3.25 ppm to 4.19 ppm predicted to be the product of deshielding by formation of an aromatic 1,2,3-triazole, and confirmed by a combination of 1D and 2D NMR experiments (Supporting Information below). In addition, signal integrations of the newly formed adduct were congruent with those of the unreacted polymer, also confirming conjugation was complete.

Click reactions, in particular 1,3-dipolar cycloadditions of azides and alkynes, can be used for development of new and robust conjugation approaches to commonly inert, yet environmentally attractive, PHA biopolyesters. This example demonstrated the ability of the E. coli LSBJ strain to produce a panel of novel and unnatural MCL PHA homo- and copolymers containing chemically tractable moieties from synthetic azidofatty acid feedstocks. Having access to these materials serves as an exciting entry point to diverse applications in promising fields of research such as targeted drug delivery, advanced biomaterials, polymer composites, and tissue engineering, to name a few (FIG. 37) (41,42).

Example 4 References

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Supporting Information: Figures, Materials and Methods, Synthetic Procedures, Characterization Data, and Supplementary References

This section discloses descriptions of supporting figures, materials and methods, synthetic procedures, characterization data (including NMR spectra, calibration curves, GPC traces, FT-IR spectra, and DSC/TGA thermograms), figure legends, and supplementary references for the Example 4 above.

Description of Supporting Figures

FIG. 41. NMR assignment and structural confirmation of PHODN₃-BCN—OH: ¹H NMR

FIG. 42. NMR assignment and structural confirmation of PHODN₃-BCN—OH: COSY.

FIG. 43. NMR assignment and structural confirmation of PHODN₃-BCN—OH: HSQC with adiabatic bilevel decoupling.

FIG. 44. NMR assignment and structural confirmation of PHODN₃-BCN—OH: 1D TOCSY (irradiated from =3.78-3.63 ppm).

FIGS. 45A-B. NMR spectra of Azido-PHA homopolymers. A. PHON₃. B. PHDN₃. and PHDDN₃.

FIGS. 46A-C. The mol ratio of repeating units in azido-PHA samples in relation to the starting fatty acid substrate ratio. A. PHOON₃. B. PHODN₃. C. PHODDN₃.

FIGS. 47A-B. Isolated yields of azido-PHA samples. A. PHOON₃ and PHODN₃. B. PHODDN₃.

FIGS. 48A-B. Molecular weight determinations of azido-PHA homo- and copolymers. A. PHON₃, PHDN₃, PHDDN₃ and PHOON₃. B. PHODN₃ and PHODDN₃.

FIG. 49. Representative FT-IR spectrum of azido-PHAs

FIG. 50. Thermal properties of PHOON₃., PHODN₃, and PHODDN₃.

General Experimental

Chemical reactions were carried out under argon atmosphere with dry solvents, and oven-dried glassware under anhydrous conditions unless specified otherwise. Acetone (Fisher, ACS grade), acetonitrile (MeCN, Sigma-Aldrich, ACS grade), chloroform (CHCl3, Fisher, Optima grade), methanol (MeOH, Pharmco-AAPER, HPLC grade), dichloromethane (DCM, Fisher, ACS grade), 2-propanol (iPrOH, Pharmco-AAPER, ACS grade), diethyl ether (Et₂O, Pharmco-AAPER, ACS grade, BHT stabilized), toluene (MePh, Pharmco-AAPER, ACS grade), and dimethylformamide (DMF, Acros, SPPS grade) were purchased and employed without further purification. Solvents such as ethyl acetate (EtOAc, Fisher, ACS grade) and hexanes (Fisher, ACS grade), employed in workup and chromatographic separations, were used without further purification, unless otherwise stated. Brine refers to a saturated aqueous solution of sodium chloride (NaCl, Sigma-Aldrich). Magnesium sulfate (J.T. Baker), sodium hydroxide (Fisher), glacial acetic acid (AcOH, Pharmco-AAPER, reagent grade), hydrobromic acid (HBr, 47-49%, Alfa Aesar), 10-bromodecanoic acid (Fisher), 1,8-octanediol (Acros), 1,12-dodecanediol (Fisher), N-methylmorpholine N-oxide (NMO, Acros), tetrapropylammonium perruthenate (TPAP, Acros), (1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-ylmethanol (11, BCN—OH, Sigma-Aldrich), and sodium azide (Acros) were purchased at the highest commercial quality and used without further purification, unless otherwise stated. Yields refer to chromatographically and spectroscopically (¹H and ¹³C NMR) homogeneous materials, unless otherwise stated.

Reactions were monitored by analytical thin-layer chromatography (TLC) and carried out on 250 um E. Merck silica gel plates (60F-254) using UV light as the visualizing agent and/or an acidic solution of p-anisaldehyde (PA) and heat as developing agents. Flash column chromatography was performed with SiliCycle SiliaFlash F60 silica gel (pore size 60 Å, particle size 40-63 μm).

NMR spectra were recorded on Bruker AVANCE III HD 800 MHz equipped with a 5 mm triple resonance z-gradient cryoprobe or Bruker AVANCE III 600 MHz equipped with a 5 mm Prodigy BBO cryoprobe instruments, and were calibrated using residual undeuterated solvents as internal reference (chloroform, 67 =7.26 ppm, ¹H NMR; 77.00 ppm, ¹³C NMR). Chemical shifts (δ) are reported in parts per million (ppm); NMR peak multiplicities are denoted by the following abbreviations: s=singlet, d=doublet, t=triplet, q=quartet, p=pentet, dd=doublet of doublets, dt=doublet of triplets, m=multiplet, br=broad; coupling constants (J) are reported in Hertz (Hz). Spectra were processed with Bruker TopSpin v3.5pI2.

Bacterial Strains, Plasmids, and Growth Medium

E. coli LSBJ carrying the plasmid pBBR-C1J4SII (1), was selectively grown in Lennox Broth (LB, BD Difco™) liquid medium (composition per liter: 10 g tryptone, 5 g yeast extract, and 5 g sodium chloride) while the antibiotic kanamycin sulfate was purchased from Fisher Scientific. Growth media was supplemented with kanamycin at a final concentration of 50 ug mL⁻¹ unless otherwise stated. Synthetic azidofatty acids and sodium octanoate (Sigma-Aldrich) were employed as cosubstrates for PHA biosynthesis to a total fatty acid concentration of 10 mM. To aid dissolution of fatty acids, the surfactant Brij-35 (Sigma-Aldrich) was added to flasks at a 4.0 g L⁻¹ final concentration. HPLC grade methanol and chloroform were used in sample pretreatment, extraction, and purification of polymers from dried bacterial cells.

Experimental Procedures

Synthesis of Azidofatty Acids

FIG. 34, Scheme 1 sets forth the synthesis of azidofatty acids.

8-Bromo-1-octanol (2)

The monobromination was performed as described by Chong et al. with minor modifications (2). Briefly, in a round bottom flask, diol 1 (8.16 g, 55.8 mmol) and HBr (8.00 mL, 66.4 mmol, 1.19 equiv) were refluxed in toluene (125 mL) for 24 hours. The reaction was quenched with NaOH (100 mL, 1 M) and extracted with Et₂O (3×100 mL). The organic extracts were combined, dried with brine (100 mL) and MgSO₄, and concentrated in vacuo.

To purify, excess toluene was first removed via flash column chromatography using 100% hexanes, after which 2 was collected by increasing the solvent system's polarity to 100% ethyl acetate. Pure fractions were concentrated in vacuo to yield pure 2 as a light yellow oil (9.90 g, 47.3 mmol, 72%). The spectroscopic data for 2 is identical to that previously reported by Becker et al. (3).

Analytical Data for 8-Bromo-1-octanol (2)

M.W.: 209.13 g/mol

TLC: R_(f) 0.38 (30% EtOAc/Hexane) [SiO₂, PA]

¹H NMR: (600 MHz, CDCl₃):

-   -   δ=3.62 (t, J=7.2 Hz, 2 H), 3.39 (t, J=6.6 Hz, 2 H), 1.84 (p,         J=7.2 Hz, 2 H), 1.55 (p, J=7.2 Hz, 2 H), 1.42 (q, J=6.6 Hz, 2         H), 1.33 (m, 6 H) ppm

¹³C NMR (150 MHz, CDCl₃):

-   -   δ=62.91, 33.92, 32.74, 32.67, 29.17, 28.67, 28.05, 25.60 ppm

8-Azido-1-octanol (3)

The procedure outlined by Ardes-Guisot et al. was followed with minor modifications (4). In a round bottom flask, 8-bromo-1-octanol (2, 9.90 g, 47.3 mmol) and sodium azide (6.16 g, 94.7 mmol, 2.0 equiv) were dissolved in DMF (47.3 mL), and stirred at room temperature for 52 h. After completion, the reaction was diluted with water (20 mL), and extracted with DCM (5×10 mL). The organic layers were combined and washed with brine (5×10 mL), dried over MgSO₄ and concentrated in vacuo. The crude product was purified by flash column chromatography (SiO₂, 20% acetone/hexanes), and concentrated in vacuo to yield 3 as a yellow oil (4.85 g, 28.3 mmol, 60%). The spectroscopic data obtained for 3 is as previously reported by Cao et al. (5)

Analytical Data for 8-Azido-1-octanol (3)

M.W.: 171.24 g/mol

TLC: R_(f) 0.38 (30% EtOAc/Hexane) [SiO₂, PA]

¹H NMR: (600 MHz, CDCl₃):

-   -   δ=3.64 (t, J=6.6 Hz, 2 H), 3.25 (t, J=7.2 Hz, 2 H), 1.58 (m, 5         H), 1.35 (m, 9 H), 1.41 (m, 2 H), 1.31 (m, 18 H) ppm

¹³C NMR (150 MHz, CDCl₃):

-   -   δ=63.00, 51.50, 32.71, 29.23, 29.10, 28.80, 26.62, 25.62 ppm

8-Azidooctanoic acid (4)

The following procedure was performed as described by Schmidt et al. with a few modifications (6). In a round bottom flask, NMO (9.64 g, 82.3 mmol, 12.0 equiv) and 3 (1.17 g, 6.86 mmol) were dissolved in MeCN (27 mL) while stirring at room temperature TPAP (241 mg, 0.686 mmol, 0.10 equiv) was added to mixture, and reaction was allowed to proceed for 24 h while monitoring its completion by TLC.

The reaction was quenched with excess iPrOH and concentrated in vacuo. To remove the ruthenium catalyst, the crude was dissolved and filtered through a plug of silica (1% AcOH/EtOAc), and following solvent evaporation, the product was purified by flash column chromatography (SiO₂, 30% EtOAc/hexanes containing 1% AcOH) and concentrated in vacuo to yield 4 as a yellow oil (1.04 g, 5.61 mmol, 82%). The spectroscopic data for 4 is as previously described in Colombano et al. (7).

Analytical Data for 8-Azidooctanoic acid (4)

M.W.: 185.23 g/mol

TLC: R_(f) 0.40 (30% EtOAc/Hexane) [SiO₂, PA]

¹H NMR: (600 MHz, CDCl₃):

-   -   δ=3.25 (t, J=7.2 Hz, 2 H), 3.34 (t, J=7.2 Hz, 2 H), 1.63 (p,         J=7.2 Hz, 2 H), 1.59 (p, J=7.2 Hz, 2 H), 1.36 (m, 6 H) ppm

¹³C NMR (150 MHz, CDCl₃):

-   -   δ=180.13, 51.38, 33.95, 28.82, 28.72, 26.47, 24.48 ppm

10-Azidodecanoic acid (6)

The following procedure was performed as described previously by Nagarajan et al. with slight modifications (8). 10-Bromodecanoic acid (5, 2.40 g, 9.0 mmol) and sodium azide (0.878 g, 13.5 mmol, 1.5 equiv) were dissolved in 50 mL DMF. The reaction was heated to 60° C. and stirred overnight under argon.

After cooling to room temperature, the reaction was diluted with ethyl acetate (EtOAc, 40 mL) and water (40 mL). Extracted with EtOAc (3×40 mL) and the organic layers were combined, dried with brine (2×40 mL), and magnesium sulfate. The solvent was removed in vacuo and the crude purified by flash column chromatography (SiO₂, 30% EtOAc/hexanes). After evaporation of the solvent under low pressure, 6 was obtained as a clear oil (1.67 g, 7.8 mmol, 87% yield). The spectroscopic data for 6 is identical to that previously reported by Nagarajan et al. (8).

Analytical data for 10-Azidodecanoic acid (6)

M.W.: 213.28

TLC: R_(f) 0.44 (30% EtOAc/hexanes) [SiO₂, PA]

¹H NMR: (600 MHz, CDCl₃)

-   -   δ 3.24 (t, J=7.2 Hz, 2 H), 2.34 (t, J=7.2 Hz, 2 H), 1.63 (p,         J=7.2 Hz, 2 H), 1.59 (p, J=7.2 Hz, 2 H), 1.36-1.25 (m, 10 H) ppm

¹³C NMR: (150 MHz, CDCl₃)

-   -   δ 180.30, 51.42, 34.02, 29.18, 29.04, 29.00, 28.93, 28.77,         28.01, 26.72, 26.62, 24.57 ppm

12-Bromo-1-dodecanol (8)

The monobromination was performed as described by Chong et al. with minor modifications (2). In a round bottom flask, 1,12-dodecanediol (7, 5.08 g, 24.8 mmol) and HBr (3.32 mL, 1.17 equiv) were dissolved in toluene (72.9 mL), and the reaction refluxed for 24 hours while monitoring by TLC. The reaction was quenched with NaOH (100 mL, 1 M) and extracted with Et₂O (3×50 mL). The organic layers were combined, dried with brine (100 mL) and MgSO₄ before concentrating in vacuo.

Toluene was removed via flash column chromatography (SiO₂, 100% hexanes) and, once removed, 8 was eluted by increasing the solvent system to 100% EtOAc. Pure fractions were concentrated in vacuo to yield 8 as light brown crystals (5.47 g, 20.6 mmol, 83%). The spectroscopic data for 8 is identical to that previously reported by Shorey et al. (9).

Analytical data for 12-Bromo-1-dodecanol (8)

M.W.: 265.24 g/mol

TLC: R_(f) 0.48 (30% EtOAc/hexanes) [SiO₂, PA]

¹H NMR: (600 MHz, CDCl₃):

-   -   δ=3.63 (t, J=6.6 Hz, 2 H), 3.40 (t, J=6.6 Hz, 2 H), 1.85 (p,         J=13.8 Hz, 2 H), 1.56 (p, J=13.8 Hz, 2 H), 1.44-1.39 (m, 3 H),         1.37-1.25 (m, 14 H) ppm

¹³C NMR (150 MHz, CDCl₃):

-   -   δ=62.60, 33.78, 32.68, 32.56, 29.43, 29.38, 29.35, 29.28, 29.27,         28.60, 28.01, 25.61 ppm

12-Azido-1-dodecanol (9)

The following procedure was performed as described previously by Johnstone et al. with modifications (10). In a round bottom flask, 12-bromo-1-dodecanol (8, 4.70 g, 17.7 mmol) and sodium azide (2.31 g, 35.4 mmol, 2.0 equiv) were dissolved in DMF (17.7 mL), and stirred at room temperature for 52 h. After completion, the reaction was diluted with water (20 mL), and extracted with DCM (5×10 mL). The organic layers were combined, dried with brine (5×10 mL) and MgSO₄, and concentrated in vacuo. The crude product was purified by flash column chromatography (SiO₂, 20% ethyl acetate/hexanes), and concentrated in vacuo to yield 9 as a yellow oil (2.15 g, 9.46 mmol, 53%). Spectral data was in accord with the reported values by Murozuka et al. (11).

Analytical data for 12-Azido-1-dodecanol (9)

M.W.: 227.35 g/mol

TLC: R_(f) 0.42 (30% EtOAc/hexanes) [SiO₂, PA]

¹H NMR: (600 MHz, CDCl₃):

-   -   δ=3.64 (t, J=7.2 Hz, 2 H), 3.25 (t, J=7.8 Hz, 2 H), 1.58 (m, 4         H), 1.32 (m, 18 H) ppm

¹³C NMR (150 MHz, CDCl₃):

-   -   δ=63.08, 51.50, 32.81, 29.56, 29.51, 29.49, 29.44, 29.40, 29.13,         28.83, 26.70, 25.73 ppm

12-Azidododecanoic acid (10)

Oxidation of 9 was performed as described by Schmidt et al with modifications (6). 12-Azido-1-dodecanol (9, 1.22 g, 5.37 mmol) and NMO (9.64 g, 82.3 mmol, 12.0 equiv) were dissolved in MeCN (27 mL), followed by TPAP (241 mg, 0.69 mmol, 0.13 equiv), and the reaction was stirred for 24 h at room temperature. The reaction was quenched with excess iPrOH and concentrated in vacuo. To remove the ruthenium catalyst, the crude was dissolved and filtered through a plug of silica (1% AcOH/EtOAc), and following solvent evaporation, the product was purified by flash column chromatography (SiO₂, 30% EtOAc/hexanes containing 1% AcOH) and concentrated in vacuo to yield 10 as a pale yellow oil (1.07 g, 4.43 mmol, 82%). The spectroscopic data for 10 is identical to that previously reported by Hang et al. (12).

Analytical data for 12-Azidododecanoic acid (10)

M.W.: 241.34 g/mol

TLC: R_(f) 0.44 (30% EtOAc/hexanes) [SiO₂, PA]

¹H NMR: (600 MHz, CDCl₃):

-   -   δ=3.25 (t, J=6.6 Hz, 2 H), 2.34 (t, J=7.2 Hz, 2 H), 1.63 (p,         J=14.4 Hz, 2 H), 1.59 (p, J=14.4 Hz, 2 H), 1.33 (m, 14 H) ppm

¹³C NMR (150 MHz, CDCl₃):

-   -   δ=179.3, 51.49, 33.90, 29.41 (2C), 29.34, 29.18, 29.11, 29.02,         28.83, 26.70, 24.66 ppm

PHODN₃-BCN—OH

PHODN₃ (1:0.33 C8: C10N₃, 19 mg, 0.33 μmol), containing 34 μmol azide groups, was dissolved in CDCl₃ (1.0 mL). BCN—OH (11, 5.1 mg, 34 μmol, 1.0 equiv) was added and thoroughly mixed to homogeneity, and allowed to proceed overnight at room temperature. The mixture was transferred to a clean NMR tube and analyzed by collecting 1D and 2D NMR spectra.

Analytical data for PHODN₃-BCN—OH

¹H NMR: (600 MHz, CDCl₃):

-   -   δ=5.18-5.16 (br m, 2 H, C8-3, C10N₃-3), 4.19 (br t, J=7.2 Hz, 2         H, C10N₃-10), 3.76-3.64 (m, 2 H, BCN-1), 3.09 (br m, 1H,         BCN-7a), 2.93-2.83 (br m, 2H, BCN-7b, BCN-8a), 2.69-2.65 (br m,         1H, BCN-8b), 2.58-2.47 (m, 4H, C8-2, C10N₃-2), 2.29-2.25 (m, 1H,         BCN-5a), 2.24-2.19 (br m, 1H, BCN-6a), 1.82-1.74 (br m, 2H,         C10N₃-9), 1.63-1.49 (br m, 6 H, C8-4, C10N₃-4, BCN-5b, BCN-6b),         1.34-1.23 (br m, 14H, C8-5, C8-6, C8-7, C10N₃-5, C10N₃-6,         C10N₃-7, C10N₃-8), 1.21-1.17 (m, 2H, BCN-2), 1.08-0.96 (m, 2H,         BCN-3, BCN-4), 0.89-0.84 (br m, 3H, C8-8) ppm

PHA Production and Characterization

Fermentation Conditions

E. coli LSBJ was made chemically competent, transformed with the pBBR-C1J4SII plasmid following standard procedures (13), and grown on LB-kanamycin plates at 37° C. for 12-16 h. For polymer production, as previously described (14), a single colony was used to inoculate 50 mL of sterile LB with kanamycin, and this culture was incubated at 37° C. in an orbital shaker at 250 rpm for 12-16 h (overnight). In triplicate, aliquots (1 mL) of the 50 mL E. coli LSBJ/pBBR-C1J4SII culture were used to inoculate 100 mL growth media in 500 mL baffled shake flasks. The PHA production media contained sterile LB, kanamycin, fatty acid cosubstrates, and Brij-35 as outlined above, and was incubated at 30° C. in an orbital shaker at 250 rpm for 48 hours.

Isolation and Purification

Cells were harvested by centrifugation (3,452×g, 30 min, room temperature). Following decantation, the pelleted cells were washed by resuspending in 35% (v/v) ethanol (10 mL), collected by centrifugation, frozen at −80° C., and lyophilized for 24 h. Polymer was extracted from the dried cell sample after transferring into a KIMAX™ screw thread tube, resuspending with CHCl₃ (6 mL), and heating to 100° C. for 2 h. After cooling to room temperature, the solution was syringe filtered (PTFE, 0.45 μm) into a 20 mL scintillation vial, and evaporated solvent in vacuo to yield a pale yellow crude polymer sample. To purify, the crude sample was redissolved in CHCl₃ (2 mL) and added dropwise to a chilled (−20° C.), and well-stirred MeOH (10 mL) solution. The cloudy solution was centrifuged (3,452×g, 30 min, 4° C.) to pellet the polymer sample, washed by resuspending the sample after decantation with an additional 5 mL of MeOH, and centrifuged (3,452×g, 30 min, 4° C.). Following decantation and dissolution of the pellet with CHCl₃ (2 mL), the polymer solution was concentrated in a rotary evaporator, and full evaporation of residual solvent was accomplished under high vacuum overnight. Purified PHA samples were kept at −20° C. pending further analyses.

Molecular Weight Determination

Both weight average (M_(w)) and number average (M_(n)) molecular weights were determined using gel permeation chromatography as described previously (18). Polymer samples were dissolved in chloroform to an approximate concentration of 1.0 g L⁻¹ and passed through a 0.45 μm PTFE syringe filter. Samples were injected at a volume of 50 μl into a Shimadzu LC-20AD liquid chromatograph equipped with a Shimadzu SIL-20A autosampler, a Shimadzu CTO-20A column oven, and a Shimadzu RID-10A refractive index detector. Samples were passed through an 8×50 mm styrenedivinylbenvene (SDV) guard column (5 μm particles; Polymer Standards Service) and an 8×300 mm SDV analytical column (5 μm particles; mixed bed porosity; max molecular weight 1E6 Da; Polymer Standards Service product sda083005lim). The column oven was maintained at 40° C., and chloroform was used as the mobile phase at 1 mL min⁻¹. Polystyrene molecular weight standards with a narrow polydispersity index were employed for calibration. Analysis was performed using Shimadzu LCsolution software.

Thermal Analysis

The decomposition temperatures (T_(d1) and T_(d2)) of extracted azido-PHA polymers were determined by thermogravimetric analysis (TGA). The melting temperatures (T_(m)), crystallization temperatures (T_(c)), and glass-transition temperatures (T_(g)) of the polymers were determined by differential scanning calorimetry (DSC). TGA experiments were performed on a TGA Q5000IR (TA Instruments). Approximately 2-8 mg of extracted polymer sample was heated under nitrogen atmosphere at 10° C. min⁻¹ to 900° C. T_(d1) and T_(d2) were taken at the initiation of sample degradation events. DSC experiments were performed on a DSC Q200 (TA Instruments). Between 2-4 mg of polymer sample were heated to 200° C. at 10° C. min⁻¹, cooled to −80° C. at 5° C. min⁻¹, and then heated back to 200° C. at 10° C. min⁻¹. The T_(m) and T_(c) were taken at the bottom or top of their peaks, respectively, if present. The T_(g) was taken in the middle of the transition. Analyses for DSC and TGA data were performed with TA Instruments Universal Analysis 2000 software.

Infrared Spectroscopy

Experiments were performed in a Bruker Tensor 27 FT-IR spectrometer equipped with an attenuated total reflection (ATR) stage. To confirm the presence of the azide functional group on copolymer samples, the stretching vibration at ˜2150 cm⁻¹ was detected (15). Samples were scanned 16 times and the data analyzed in transmittance mode with the Bruker OPUS 6.5 software.

Supplementary References for Example 4 Supporting Information

-   (1) Example 1. -   (2) Chong, J. M.; Heuft, M. A.; Rabbat, P. Solvent Effects on the     Monobromination of α,ω-Diols: A Convenient Preparation of     w-Bromoalkanols. J. Org. Chem. 2000, 65, 5837-5838. -   (3) Becker, J.; Butt, L.; von Kiedrowski, V.; Mischler, E.; Quentin,     F.; Hiersemann, M. Total Synthesis of (−)-Ecklonialactone B. Org.     Lett. 2013, 15, 5982-5985. -   (4) Ardes-Guisot, N.; Alonzi, D. S.; Reinkensmeier, G.; Butters, T.     D.; Norez, C.; Becq, F.; Shimada, Y.; Nakagawa, S.; Kato, A.;     Blériot, Y.; Sollogoub, M.; Vauzeilles, B. Selection of the     Biological Activity of DNJ Neoglycoconjugates through Click Length     Variation of the Side Chain. Org. Biomol. Chem. 2011, 9, 5373-5388. -   (5) Cao, B.; White, J. M.; Williams, S. J. Synthesis of     Glycoconjugate Fragments of Mycobacterial Phosphatidylinositol     Mannosides and Lipomannan. Beilstein J. Org. Chem. 2011, 7, 369-377. -   (6) Schmidt, A.-K. C.; Stark, C. B. W. TPAP-Catalyzed Direct     Oxidation of Primary Alcohols to Carboxylic Acids through Stabilized     Aldehyde Hydrates. Org. Lett. 2011, 13, 4164-4167. -   (7) Colombano, G.; Travelli, C.; Galli, U.; Caldarelli, A.;     Chini, M. G.; Canonico, P. L.; Sorba, G.; Bifulco, G.; Tron, G. C.;     Genazzani, A. A. A Novel Potent Nicotinamide     Phosphoribosyltransferase Inhibitor Synthesized via Click     Chemistry. J. Med. Chem. 2010, 53, 616-623. -   (8) Nagarajan, S. R.; Devadas, B.; Zupec, M. E.; Freeman, S. K.;     Brown, D. L.; Lu, H. F.; Mehta, P. P.; Kishore, N. S.; McWherter, C.     A.; Getman, D. P.; Gordon, J. I.; Sikorski, J. A. Conformationally     Constrained [p-(ω-Aminoalkyl)phenacetyl]-L-Seryl-L-Lysyl Dipeptide     Amides as Potent Peptidomimetic Inhibitors of Candida Albicans and     Human Myristoyl-CoA:protein N-Myristoyl Transferase. J. Med. Chem.     1997, 40, 1422-1438. -   (9) Shorey, B. J.; Lee, V.; Baldwin, J. E. Synthesis of the Arctic     Sponge Alkaloid Viscosaline and the Marine Sponge Alkaloid     Theonelladin C. Tetrahedron 2007, 63, 5587-5592. -   (10) Johnstone, K. D.; Karoli, T.; Liu, L.; Dredge, K.; Copeman, E.;     Li, C. P.; Davis, K.; Hammond, E.; Bytheway, I.; Kostewicz, E.;     Chiu, F. C. K.; Shackleford, D. M.; Charman, S. A.; Charman, W. N.;     Harenberg, J.; Gonda, T. J.; Ferro, V. Synthesis and Biological     Evaluation of Polysulfated Oligosaccharide Glycosides as Inhibitors     of Angiogenesis and Tumor Growth. J. Med. Chem. 2010, 53, 1686-1699. -   (11) Murozuka, Y.; Kasuya, M. C. Z.; Kobayashi, M.; Watanabe, Y.;     Sato, T.; Hatanaka, K. Efficient Sialylation on Azidododecyl     Lactosides by Using B16 Melanoma Cells. Chem. Biodivers. 2005, 2,     1063-1078. -   (12) Hang, H. C.; Geutjes, E.-J.; Grotenbreg, G.; Pollington, A. M.;     Bijlmakers, M. J.; Ploegh, H. L. Chemical Probes for the Rapid     Detection of Fatty-Acylated Proteins in Mammalian Cells. J. Am.     Chem. Soc. 2007, 129, 2744-2745. -   (13) Sambrook, J.; Russell, D. W. Molecular Cloning: A Laboratory     Manual, Volume 1; CSHL Press, 2001. -   (14) Example 2. -   (15) Lieber, E.; Rao, C. N. R.; Chao, T. S.; Hoffman, C. W. W.     Infrared Spectra of Organic Azides. Anal. Chem. 1957, 29, 916-918.

6.5 Example 5: Uses of PHA Polymers

This example describes uses for PHA polymers.

Production of PHA_(scl/mcl)-co-PHX and Multivalent Copolymers

To broaden the physical properties and application spectrum of PHAs, a panel of PHA copolymers was designed, produced and characterized. The PHA copolymers had the form of PHA_(scl/mcl)co-PHX and multivalent PHA_(scl/mcl)co-PHX-co-PHX, where “PHA_(scl/mcl)” represents either a base monomer encompassed by SCL fatty acids (C3-05) or MCL fatty acids (C6-C14), and “PHX” represents MCL fatty acids of variable chain lengths containing either a terminal alkene (X=U_(ene)), alkyne (X=U_(yne)) or azide (X=N₃) moiety. These functional MCL fatty acids enable PHA copolymers to be orthogonally conjugated via thiol-ene click reactions (alkenes/alkynes) and standard CuAAC or SPAAC reactions (alkynes/azides).

Targeted Drug Delivery

After bacterial synthesis of PHA polymers, functionalized targeting agents were added by click chemistry to improve selectivity for an assortment of pathologies (FIG. 38). The functionalized PHA polymers have been formulated into nanoparticles via miniemulsion and/or nano-precipitation, and characterized by dynamic light scattering and atomic force microscopy (AFM).

Tissue Engineering: Small oligopeptides may be used to induce cellular proliferation on tissue engineering scaffolds. The RGD signal peptide has been shown to important cellular recognition and to aid in cellular attachment. To provide a tissue engineering scaffold for guided cellular attachment, we functionalized click-ready PHAs as follows.

A dibenzocyclooctyne-Arg-Gly-Asp (DBCO-RGD) peptide was synthesized as previously described (Wang, X., Gobbo, P., Suchy, M., Workentin, M. S., and Hudson, R. H. E. 2014). Peptide-decorated gold nanoparticles via strain-promoted azide alkyne cycloaddition and post assembly deprotection (RSC Advances. 4. 43087-43091). This was conjugated via a SPAAC to the PHA-azide polymers as described above application for BCN—OH.

Imaging

Clickable PHAs can be used as imaging agents. In this example, was attached to dibenzylcyclooctyne-Fluor 568 (DBCO-5) to the azido-PHA nanoparticles as shown in FIG. 39.

Visualization of nanoparticles in cells was done in a manner similar to that previously described (Wilson, D.R., Zhang, N., Silvers, A.L., Forstner, M. B., and Bader, R. A. 2014. Synthesis and evaluation of cyclosporine A-loaded polysialic acid-polycaprolactone micelles for rheumatoid arthritis. European J of Pharmaceutical Sciences. 51. 146-156). The resultant Azido-PHA-DBCO-5 nanoparticles are shown to be preferentially incorporated into MDA-MB231 cells (P13) (FIG. 40, right panel).

Biotinylated PHAs

As a demonstration of attaching biotin derivatives to PHAs, dibenzocylooctyne-PEG4-biotin (DBCO-PEG4-biotin) was attached the azido PHAs to produce azido-PHA-DBCO-PEG4 biotin. Azido-PHA-DBCO-PEG4 biotin nanoparticles can be used as nucleic acid carriers for transformation of cells.

The present invention is not to be limited in scope by the specific embodiments described herein. Indeed, various modifications of the invention in addition to those described herein will become apparent to those skilled in the art from the foregoing description. Such modifications are intended to fall within the scope of the appended claims.

While embodiments of the present disclosure have been particularly shown and described with reference to certain examples and features, it will be understood by one skilled in the art that various changes in detail may be effected therein without departing from the spirit and scope of the present disclosure as defined by claims that can be supported by the written description and drawings. Further, where exemplary embodiments are described with reference to a certain number of elements it will be understood that the exemplary embodiments can be practiced utilizing either less than or more than the certain number of elements.

All references cited herein are incorporated herein by reference in their entirety and for all purposes to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated by reference in its entirety for all purposes.

The citation of any publication is for its disclosure prior to the filing date and should not be construed as an admission that the present invention is not entitled to antedate such publication by virtue of prior invention. 

What is claimed is:
 1. A system for producing a prescribed unit size azido-poly(3-hydroxyalkanoate) (azido-PHA) polymer comprising a recombinant bacterium, wherein the bacterium: a. metabolizes short to long chain fatty acids without induction, and b. expresses an (R)-specific enoyl-CoA hydratase and a PHA synthase, wherein the bacterium is fed a fatty acid substrate, wherein the fatty acid substrate is a ω-azido-fatty acid substrate, the ω-azido-fatty acid comprising a terminal azide, and the ω-azido-fatty acid being of equal carbon length to the prescribed unit size of the azido-PHA polymer to be produced.
 2. The system of claim 1, wherein the bacterium is not a native or natural producer of PHA or lacks enzymes to degrade PHA once synthesized.
 3. The system of claim 1, wherein the recombinant bacterium is a recombinant E. coli.
 4. The system of claim 1, wherein the recombinant bacterium comprises a plasmid comprising an (R)-specific enoyl-CoA hydratase gene and a PHA synthase gene. 